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Candida species isolated from clinical samples in a tertiary hospital in Honduras: Where is Candida auris?

BACKGROUND AND PURPOSE: Infections by emerging and multiresistant Candida species are becoming more frequent throughout the world. This study aimed to describe Candida species in different wards of a tertiary hospital in Honduras. MATERIALS AND METHODS: The prevalence of species within the C. albica...

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Autores principales: Ortiz, Bryan, Aguilar, Kateryn, Galindo, Celeste, Molina, Lizzy, Fontecha, Gustavo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Society of Medical Mycology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10084484/
https://www.ncbi.nlm.nih.gov/pubmed/37051554
http://dx.doi.org/10.18502/cmm.8.3.11212
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author Ortiz, Bryan
Aguilar, Kateryn
Galindo, Celeste
Molina, Lizzy
Fontecha, Gustavo
author_facet Ortiz, Bryan
Aguilar, Kateryn
Galindo, Celeste
Molina, Lizzy
Fontecha, Gustavo
author_sort Ortiz, Bryan
collection PubMed
description BACKGROUND AND PURPOSE: Infections by emerging and multiresistant Candida species are becoming more frequent throughout the world. This study aimed to describe Candida species in different wards of a tertiary hospital in Honduras. MATERIALS AND METHODS: The prevalence of species within the C. albicans complex was estimated using a molecular approach, and C. auris was investigated using a yeast pool-based DNA extraction method. In total, 328 yeast isolates were identified using phenotypic approaches. For the identification of species within the C. albicans complex, a molecular approach based on the size polymorphisms of the hpw1 gene was used. In addition, a technique was optimized based on DNA extraction in pools for the rapid identification of C. auris. RESULTS: A total of 11 species of Candida were identified in the hospital wards. C. albicans showed the highest number of isolates (52.4%). Within the C. albicans complex, C. albicans sensu stricto was the most common, followed by C. dubliniensis. However, C. auris was not found. CONCLUSION: Reports on the distribution of Candida species in Honduras are limited; accordingly, the data from this study are of importance for a better understanding of their epidemiology. Moreover, a simple method was offered for the detection of C. auris that could help in its detection in low-resource settings.
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spelling pubmed-100844842023-04-11 Candida species isolated from clinical samples in a tertiary hospital in Honduras: Where is Candida auris? Ortiz, Bryan Aguilar, Kateryn Galindo, Celeste Molina, Lizzy Fontecha, Gustavo Curr Med Mycol Original Article BACKGROUND AND PURPOSE: Infections by emerging and multiresistant Candida species are becoming more frequent throughout the world. This study aimed to describe Candida species in different wards of a tertiary hospital in Honduras. MATERIALS AND METHODS: The prevalence of species within the C. albicans complex was estimated using a molecular approach, and C. auris was investigated using a yeast pool-based DNA extraction method. In total, 328 yeast isolates were identified using phenotypic approaches. For the identification of species within the C. albicans complex, a molecular approach based on the size polymorphisms of the hpw1 gene was used. In addition, a technique was optimized based on DNA extraction in pools for the rapid identification of C. auris. RESULTS: A total of 11 species of Candida were identified in the hospital wards. C. albicans showed the highest number of isolates (52.4%). Within the C. albicans complex, C. albicans sensu stricto was the most common, followed by C. dubliniensis. However, C. auris was not found. CONCLUSION: Reports on the distribution of Candida species in Honduras are limited; accordingly, the data from this study are of importance for a better understanding of their epidemiology. Moreover, a simple method was offered for the detection of C. auris that could help in its detection in low-resource settings. Iranian Society of Medical Mycology 2022-09 /pmc/articles/PMC10084484/ /pubmed/37051554 http://dx.doi.org/10.18502/cmm.8.3.11212 Text en Copyright: © 2021, Published by Mazandaran University of Medical Sciences on behalf of Iranian Society of Medical Mycology and Invasive Fungi Research Center. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY) License ( http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ) which permits unrestricted use, distribution and reproduction in any medium, provided appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Ortiz, Bryan
Aguilar, Kateryn
Galindo, Celeste
Molina, Lizzy
Fontecha, Gustavo
Candida species isolated from clinical samples in a tertiary hospital in Honduras: Where is Candida auris?
title Candida species isolated from clinical samples in a tertiary hospital in Honduras: Where is Candida auris?
title_full Candida species isolated from clinical samples in a tertiary hospital in Honduras: Where is Candida auris?
title_fullStr Candida species isolated from clinical samples in a tertiary hospital in Honduras: Where is Candida auris?
title_full_unstemmed Candida species isolated from clinical samples in a tertiary hospital in Honduras: Where is Candida auris?
title_short Candida species isolated from clinical samples in a tertiary hospital in Honduras: Where is Candida auris?
title_sort candida species isolated from clinical samples in a tertiary hospital in honduras: where is candida auris?
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10084484/
https://www.ncbi.nlm.nih.gov/pubmed/37051554
http://dx.doi.org/10.18502/cmm.8.3.11212
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