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Candida species isolated from clinical samples in a tertiary hospital in Honduras: Where is Candida auris?
BACKGROUND AND PURPOSE: Infections by emerging and multiresistant Candida species are becoming more frequent throughout the world. This study aimed to describe Candida species in different wards of a tertiary hospital in Honduras. MATERIALS AND METHODS: The prevalence of species within the C. albica...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Iranian Society of Medical Mycology
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10084484/ https://www.ncbi.nlm.nih.gov/pubmed/37051554 http://dx.doi.org/10.18502/cmm.8.3.11212 |
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author | Ortiz, Bryan Aguilar, Kateryn Galindo, Celeste Molina, Lizzy Fontecha, Gustavo |
author_facet | Ortiz, Bryan Aguilar, Kateryn Galindo, Celeste Molina, Lizzy Fontecha, Gustavo |
author_sort | Ortiz, Bryan |
collection | PubMed |
description | BACKGROUND AND PURPOSE: Infections by emerging and multiresistant Candida species are becoming more frequent throughout the world. This study aimed to describe Candida species in different wards of a tertiary hospital in Honduras. MATERIALS AND METHODS: The prevalence of species within the C. albicans complex was estimated using a molecular approach, and C. auris was investigated using a yeast pool-based DNA extraction method. In total, 328 yeast isolates were identified using phenotypic approaches. For the identification of species within the C. albicans complex, a molecular approach based on the size polymorphisms of the hpw1 gene was used. In addition, a technique was optimized based on DNA extraction in pools for the rapid identification of C. auris. RESULTS: A total of 11 species of Candida were identified in the hospital wards. C. albicans showed the highest number of isolates (52.4%). Within the C. albicans complex, C. albicans sensu stricto was the most common, followed by C. dubliniensis. However, C. auris was not found. CONCLUSION: Reports on the distribution of Candida species in Honduras are limited; accordingly, the data from this study are of importance for a better understanding of their epidemiology. Moreover, a simple method was offered for the detection of C. auris that could help in its detection in low-resource settings. |
format | Online Article Text |
id | pubmed-10084484 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Iranian Society of Medical Mycology |
record_format | MEDLINE/PubMed |
spelling | pubmed-100844842023-04-11 Candida species isolated from clinical samples in a tertiary hospital in Honduras: Where is Candida auris? Ortiz, Bryan Aguilar, Kateryn Galindo, Celeste Molina, Lizzy Fontecha, Gustavo Curr Med Mycol Original Article BACKGROUND AND PURPOSE: Infections by emerging and multiresistant Candida species are becoming more frequent throughout the world. This study aimed to describe Candida species in different wards of a tertiary hospital in Honduras. MATERIALS AND METHODS: The prevalence of species within the C. albicans complex was estimated using a molecular approach, and C. auris was investigated using a yeast pool-based DNA extraction method. In total, 328 yeast isolates were identified using phenotypic approaches. For the identification of species within the C. albicans complex, a molecular approach based on the size polymorphisms of the hpw1 gene was used. In addition, a technique was optimized based on DNA extraction in pools for the rapid identification of C. auris. RESULTS: A total of 11 species of Candida were identified in the hospital wards. C. albicans showed the highest number of isolates (52.4%). Within the C. albicans complex, C. albicans sensu stricto was the most common, followed by C. dubliniensis. However, C. auris was not found. CONCLUSION: Reports on the distribution of Candida species in Honduras are limited; accordingly, the data from this study are of importance for a better understanding of their epidemiology. Moreover, a simple method was offered for the detection of C. auris that could help in its detection in low-resource settings. Iranian Society of Medical Mycology 2022-09 /pmc/articles/PMC10084484/ /pubmed/37051554 http://dx.doi.org/10.18502/cmm.8.3.11212 Text en Copyright: © 2021, Published by Mazandaran University of Medical Sciences on behalf of Iranian Society of Medical Mycology and Invasive Fungi Research Center. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY) License ( http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ) which permits unrestricted use, distribution and reproduction in any medium, provided appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Ortiz, Bryan Aguilar, Kateryn Galindo, Celeste Molina, Lizzy Fontecha, Gustavo Candida species isolated from clinical samples in a tertiary hospital in Honduras: Where is Candida auris? |
title | Candida species isolated from clinical samples in a tertiary hospital in Honduras: Where is Candida auris? |
title_full | Candida species isolated from clinical samples in a tertiary hospital in Honduras: Where is Candida auris? |
title_fullStr | Candida species isolated from clinical samples in a tertiary hospital in Honduras: Where is Candida auris? |
title_full_unstemmed | Candida species isolated from clinical samples in a tertiary hospital in Honduras: Where is Candida auris? |
title_short | Candida species isolated from clinical samples in a tertiary hospital in Honduras: Where is Candida auris? |
title_sort | candida species isolated from clinical samples in a tertiary hospital in honduras: where is candida auris? |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10084484/ https://www.ncbi.nlm.nih.gov/pubmed/37051554 http://dx.doi.org/10.18502/cmm.8.3.11212 |
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