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Genetic diversity of toxigenic Fusarium verticillioides associated with maize grains, India

In the present investigation, prevalence, genetic diversity, and mycotoxin producing potential of Fusarium species associated with maize grain samples were studied from different geographical regions of India. The highest prevalence of Fusarium verticillioides was recorded as 88.52%, followed by F....

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Autores principales: Navale, Vishwambar D., Sawant, Amol M., Vamkudoth, Koteswara Rao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Genética 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10084715/
https://www.ncbi.nlm.nih.gov/pubmed/37036389
http://dx.doi.org/10.1590/1678-4685-GMB-2022-0073
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author Navale, Vishwambar D.
Sawant, Amol M.
Vamkudoth, Koteswara Rao
author_facet Navale, Vishwambar D.
Sawant, Amol M.
Vamkudoth, Koteswara Rao
author_sort Navale, Vishwambar D.
collection PubMed
description In the present investigation, prevalence, genetic diversity, and mycotoxin producing potential of Fusarium species associated with maize grain samples were studied from different geographical regions of India. The highest prevalence of Fusarium verticillioides was recorded as 88.52%, followed by F. coffeatum, F. foetens, and F. euwallaceae, 6.55%, 3.27%, and 1.63%, respectively. We isolated 54 strains of F. verticillioides, and their genetic diversity was studied by inter simple sequence repeats (ISSR). The ISSR fingerprints (AG) 8C and (AG) 8G showed 252 and 368 microsatellite sites in the genome of F. verticillioides and resulted in 99-100% repeatability and reproducibility. The Simpson (SID) and Shannon (H) indices (0.78 and 2.36) suggest that F. verticillioides strains exhibit moderate to high diversity. Molecular detection of fumonisin B1 (FB1) biosynthetic genes (FUM1 and FUM13) involved in FB1 production in F. verticillioides was confirmed by polymerase chain reaction (PCR). Furthermore, 91% of the strains were positive for FB1 production, which was affirmed by liquid chromatography with tandem mass spectrometry (LC-MS-MS). In-vitro appurtenance of F. verticillioides spores exhibited a high to moderate effect on the growth and development of the maize. The current finding demonstrated that most F. verticillioides strains showed a wide range of genetic diversity with varied toxigenic and pathogenic potentials. In conclusion, for the first time, F. coffeatum, F. foetens, and F. euwallaceae species were reported from maize grain samples in India. They were positive for FB1 and negatively affecting grain quality, which is a major concern in food safety.
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spelling pubmed-100847152023-04-11 Genetic diversity of toxigenic Fusarium verticillioides associated with maize grains, India Navale, Vishwambar D. Sawant, Amol M. Vamkudoth, Koteswara Rao Genet Mol Biol Genetics of Microorganisms In the present investigation, prevalence, genetic diversity, and mycotoxin producing potential of Fusarium species associated with maize grain samples were studied from different geographical regions of India. The highest prevalence of Fusarium verticillioides was recorded as 88.52%, followed by F. coffeatum, F. foetens, and F. euwallaceae, 6.55%, 3.27%, and 1.63%, respectively. We isolated 54 strains of F. verticillioides, and their genetic diversity was studied by inter simple sequence repeats (ISSR). The ISSR fingerprints (AG) 8C and (AG) 8G showed 252 and 368 microsatellite sites in the genome of F. verticillioides and resulted in 99-100% repeatability and reproducibility. The Simpson (SID) and Shannon (H) indices (0.78 and 2.36) suggest that F. verticillioides strains exhibit moderate to high diversity. Molecular detection of fumonisin B1 (FB1) biosynthetic genes (FUM1 and FUM13) involved in FB1 production in F. verticillioides was confirmed by polymerase chain reaction (PCR). Furthermore, 91% of the strains were positive for FB1 production, which was affirmed by liquid chromatography with tandem mass spectrometry (LC-MS-MS). In-vitro appurtenance of F. verticillioides spores exhibited a high to moderate effect on the growth and development of the maize. The current finding demonstrated that most F. verticillioides strains showed a wide range of genetic diversity with varied toxigenic and pathogenic potentials. In conclusion, for the first time, F. coffeatum, F. foetens, and F. euwallaceae species were reported from maize grain samples in India. They were positive for FB1 and negatively affecting grain quality, which is a major concern in food safety. Sociedade Brasileira de Genética 2023-04-07 /pmc/articles/PMC10084715/ /pubmed/37036389 http://dx.doi.org/10.1590/1678-4685-GMB-2022-0073 Text en https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Genetics of Microorganisms
Navale, Vishwambar D.
Sawant, Amol M.
Vamkudoth, Koteswara Rao
Genetic diversity of toxigenic Fusarium verticillioides associated with maize grains, India
title Genetic diversity of toxigenic Fusarium verticillioides associated with maize grains, India
title_full Genetic diversity of toxigenic Fusarium verticillioides associated with maize grains, India
title_fullStr Genetic diversity of toxigenic Fusarium verticillioides associated with maize grains, India
title_full_unstemmed Genetic diversity of toxigenic Fusarium verticillioides associated with maize grains, India
title_short Genetic diversity of toxigenic Fusarium verticillioides associated with maize grains, India
title_sort genetic diversity of toxigenic fusarium verticillioides associated with maize grains, india
topic Genetics of Microorganisms
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10084715/
https://www.ncbi.nlm.nih.gov/pubmed/37036389
http://dx.doi.org/10.1590/1678-4685-GMB-2022-0073
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