Generation of a gene-corrected human isogenic iPSC line from an Alzheimer’s disease iPSC line carrying the London mutation in APP (V717I)

We report the genome-editing of an existing iPSC line carrying the London mutation in APP (V717I) into an iPSC line in which the pathogenic mutation was corrected. The resulting isogenic iPSC line maintained pluripotent stem cell morphology, a normal karyotype, expression of pluripotency markers and...

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Detalles Bibliográficos
Autores principales: Hernández, Damián, Schlicht, Stephanie Morgan, Daniszewski, Maciej, Karch, Celeste M., Goate, Alison M., Pébay, Alice
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10084742/
https://www.ncbi.nlm.nih.gov/pubmed/34088002
http://dx.doi.org/10.1016/j.scr.2021.102373
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author Hernández, Damián
Schlicht, Stephanie Morgan
Daniszewski, Maciej
Karch, Celeste M.
Goate, Alison M.
Pébay, Alice
author_facet Hernández, Damián
Schlicht, Stephanie Morgan
Daniszewski, Maciej
Karch, Celeste M.
Goate, Alison M.
Pébay, Alice
author_sort Hernández, Damián
collection PubMed
description We report the genome-editing of an existing iPSC line carrying the London mutation in APP (V717I) into an iPSC line in which the pathogenic mutation was corrected. The resulting isogenic iPSC line maintained pluripotent stem cell morphology, a normal karyotype, expression of pluripotency markers and the ability to differentiate into the three germ-layers in vitro.
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spelling pubmed-100847422023-04-10 Generation of a gene-corrected human isogenic iPSC line from an Alzheimer’s disease iPSC line carrying the London mutation in APP (V717I) Hernández, Damián Schlicht, Stephanie Morgan Daniszewski, Maciej Karch, Celeste M. Goate, Alison M. Pébay, Alice Stem Cell Res Article We report the genome-editing of an existing iPSC line carrying the London mutation in APP (V717I) into an iPSC line in which the pathogenic mutation was corrected. The resulting isogenic iPSC line maintained pluripotent stem cell morphology, a normal karyotype, expression of pluripotency markers and the ability to differentiate into the three germ-layers in vitro. 2021-05 2021-04-30 /pmc/articles/PMC10084742/ /pubmed/34088002 http://dx.doi.org/10.1016/j.scr.2021.102373 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Article
Hernández, Damián
Schlicht, Stephanie Morgan
Daniszewski, Maciej
Karch, Celeste M.
Goate, Alison M.
Pébay, Alice
Generation of a gene-corrected human isogenic iPSC line from an Alzheimer’s disease iPSC line carrying the London mutation in APP (V717I)
title Generation of a gene-corrected human isogenic iPSC line from an Alzheimer’s disease iPSC line carrying the London mutation in APP (V717I)
title_full Generation of a gene-corrected human isogenic iPSC line from an Alzheimer’s disease iPSC line carrying the London mutation in APP (V717I)
title_fullStr Generation of a gene-corrected human isogenic iPSC line from an Alzheimer’s disease iPSC line carrying the London mutation in APP (V717I)
title_full_unstemmed Generation of a gene-corrected human isogenic iPSC line from an Alzheimer’s disease iPSC line carrying the London mutation in APP (V717I)
title_short Generation of a gene-corrected human isogenic iPSC line from an Alzheimer’s disease iPSC line carrying the London mutation in APP (V717I)
title_sort generation of a gene-corrected human isogenic ipsc line from an alzheimer’s disease ipsc line carrying the london mutation in app (v717i)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10084742/
https://www.ncbi.nlm.nih.gov/pubmed/34088002
http://dx.doi.org/10.1016/j.scr.2021.102373
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