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RNA virus-mediated changes in organismal oxygen consumption rate in young and old Drosophila melanogaster males

Aging is accompanied by increased susceptibility to infections including with viral pathogens resulting in higher morbidity and mortality among the elderly. Significant changes in host metabolism can take place following virus infection. Efficient immune responses are energetically costly, and virus...

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Autores principales: Hagedorn, Eli, Bunnell, Dean, Henschel, Beate, Smith, Daniel L., Dickinson, Stephanie, Brown, Andrew W., De Luca, Maria, Turner, Ashley N., Chtarbanova, Stanislava
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10085608/
https://www.ncbi.nlm.nih.gov/pubmed/36947702
http://dx.doi.org/10.18632/aging.204593
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author Hagedorn, Eli
Bunnell, Dean
Henschel, Beate
Smith, Daniel L.
Dickinson, Stephanie
Brown, Andrew W.
De Luca, Maria
Turner, Ashley N.
Chtarbanova, Stanislava
author_facet Hagedorn, Eli
Bunnell, Dean
Henschel, Beate
Smith, Daniel L.
Dickinson, Stephanie
Brown, Andrew W.
De Luca, Maria
Turner, Ashley N.
Chtarbanova, Stanislava
author_sort Hagedorn, Eli
collection PubMed
description Aging is accompanied by increased susceptibility to infections including with viral pathogens resulting in higher morbidity and mortality among the elderly. Significant changes in host metabolism can take place following virus infection. Efficient immune responses are energetically costly, and viruses divert host molecular resources to promote their own replication. Virus-induced metabolic reprogramming could impact infection outcomes, however, how this is affected by aging and impacts organismal survival remains poorly understood. RNA virus infection of Drosophila melanogaster with Flock House virus (FHV) is an effective model to study antiviral responses with age, where older flies die faster than younger flies due to impaired disease tolerance. Using this aged host-virus model, we conducted longitudinal, single-fly respirometry studies to determine if metabolism impacts infection outcomes. Analysis using linear mixed models on Oxygen Consumption Rate (OCR) following the first 72-hours post-infection showed that FHV modulates respiration, but age has no significant effect on OCR. However, the longitudinal assessment revealed that OCR in young flies progressively and significantly decreases, while OCR in aged flies remains constant throughout the three days of the experiment. Furthermore, we found that the OCR signature at 24-hours varied in response to both experimental treatment and survival status. FHV-injected flies that died prior to 48- or 72-hours measurements had a lower OCR compared to survivors at 48-hours. Our findings suggest the host’s metabolic profile could influence the outcome of viral infections.
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spelling pubmed-100856082023-04-11 RNA virus-mediated changes in organismal oxygen consumption rate in young and old Drosophila melanogaster males Hagedorn, Eli Bunnell, Dean Henschel, Beate Smith, Daniel L. Dickinson, Stephanie Brown, Andrew W. De Luca, Maria Turner, Ashley N. Chtarbanova, Stanislava Aging (Albany NY) Research Paper Aging is accompanied by increased susceptibility to infections including with viral pathogens resulting in higher morbidity and mortality among the elderly. Significant changes in host metabolism can take place following virus infection. Efficient immune responses are energetically costly, and viruses divert host molecular resources to promote their own replication. Virus-induced metabolic reprogramming could impact infection outcomes, however, how this is affected by aging and impacts organismal survival remains poorly understood. RNA virus infection of Drosophila melanogaster with Flock House virus (FHV) is an effective model to study antiviral responses with age, where older flies die faster than younger flies due to impaired disease tolerance. Using this aged host-virus model, we conducted longitudinal, single-fly respirometry studies to determine if metabolism impacts infection outcomes. Analysis using linear mixed models on Oxygen Consumption Rate (OCR) following the first 72-hours post-infection showed that FHV modulates respiration, but age has no significant effect on OCR. However, the longitudinal assessment revealed that OCR in young flies progressively and significantly decreases, while OCR in aged flies remains constant throughout the three days of the experiment. Furthermore, we found that the OCR signature at 24-hours varied in response to both experimental treatment and survival status. FHV-injected flies that died prior to 48- or 72-hours measurements had a lower OCR compared to survivors at 48-hours. Our findings suggest the host’s metabolic profile could influence the outcome of viral infections. Impact Journals 2023-03-22 /pmc/articles/PMC10085608/ /pubmed/36947702 http://dx.doi.org/10.18632/aging.204593 Text en Copyright: © 2023 Hagedorn et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Hagedorn, Eli
Bunnell, Dean
Henschel, Beate
Smith, Daniel L.
Dickinson, Stephanie
Brown, Andrew W.
De Luca, Maria
Turner, Ashley N.
Chtarbanova, Stanislava
RNA virus-mediated changes in organismal oxygen consumption rate in young and old Drosophila melanogaster males
title RNA virus-mediated changes in organismal oxygen consumption rate in young and old Drosophila melanogaster males
title_full RNA virus-mediated changes in organismal oxygen consumption rate in young and old Drosophila melanogaster males
title_fullStr RNA virus-mediated changes in organismal oxygen consumption rate in young and old Drosophila melanogaster males
title_full_unstemmed RNA virus-mediated changes in organismal oxygen consumption rate in young and old Drosophila melanogaster males
title_short RNA virus-mediated changes in organismal oxygen consumption rate in young and old Drosophila melanogaster males
title_sort rna virus-mediated changes in organismal oxygen consumption rate in young and old drosophila melanogaster males
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10085608/
https://www.ncbi.nlm.nih.gov/pubmed/36947702
http://dx.doi.org/10.18632/aging.204593
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