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O(2) partitioning of sulfur oxidizing bacteria drives acidity and thiosulfate distributions in mining waters

The acidification of water in mining areas is a global environmental issue primarily catalyzed by sulfur-oxidizing bacteria (SOB). Little is known about microbial sulfur cycling in circumneutral pH mine tailing impoundment waters. Here we investigate biological sulfur oxidation over four years in a...

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Autores principales: Whaley-Martin, Kelly J., Chen, Lin-Xing, Nelson, Tara Colenbrander, Gordon, Jennifer, Kantor, Rose, Twible, Lauren E., Marshall, Stephanie, McGarry, Sam, Rossi, Laura, Bessette, Benoit, Baron, Christian, Apte, Simon, Banfield, Jillian F., Warren, Lesley A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10086054/
https://www.ncbi.nlm.nih.gov/pubmed/37037821
http://dx.doi.org/10.1038/s41467-023-37426-8
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author Whaley-Martin, Kelly J.
Chen, Lin-Xing
Nelson, Tara Colenbrander
Gordon, Jennifer
Kantor, Rose
Twible, Lauren E.
Marshall, Stephanie
McGarry, Sam
Rossi, Laura
Bessette, Benoit
Baron, Christian
Apte, Simon
Banfield, Jillian F.
Warren, Lesley A.
author_facet Whaley-Martin, Kelly J.
Chen, Lin-Xing
Nelson, Tara Colenbrander
Gordon, Jennifer
Kantor, Rose
Twible, Lauren E.
Marshall, Stephanie
McGarry, Sam
Rossi, Laura
Bessette, Benoit
Baron, Christian
Apte, Simon
Banfield, Jillian F.
Warren, Lesley A.
author_sort Whaley-Martin, Kelly J.
collection PubMed
description The acidification of water in mining areas is a global environmental issue primarily catalyzed by sulfur-oxidizing bacteria (SOB). Little is known about microbial sulfur cycling in circumneutral pH mine tailing impoundment waters. Here we investigate biological sulfur oxidation over four years in a mine tailings impoundment water cap, integrating aqueous sulfur geochemistry, genome-resolved metagenomics and metatranscriptomics. The microbial community is consistently dominated by neutrophilic, chemolithoautotrophic SOB (relative abundances of ~76% in 2015, ~55% in 2016/2017 and ~60% in 2018). Results reveal two SOB strategies alternately dominate across the four years, influencing acid generation and sulfur speciation. Under oxic conditions, novel Halothiobacillus drive lower pH conditions (as low as 4.3) and lower [S(2)O(3)(2−)] via the complete Sox pathway coupled to O(2). Under anoxic conditions, Thiobacillus spp. dominate in activity, via the incomplete Sox and rDSR pathways coupled to NO(3)(−), resulting in higher [S(2)O(3)(2−)] and no net significant acidity generation. This study provides genomic evidence explaining acidity generation and thiosulfate accumulation patterns in a circumneutral mine tailing impoundment and has significant environmental applications in preventing the discharge of sulfur compounds that can impact downstream environments. These insights illuminate opportunities for in situ biotreatment of reduced sulfur compounds and prediction of acidification events using gene-based monitoring and in situ RNA detection.
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spelling pubmed-100860542023-04-12 O(2) partitioning of sulfur oxidizing bacteria drives acidity and thiosulfate distributions in mining waters Whaley-Martin, Kelly J. Chen, Lin-Xing Nelson, Tara Colenbrander Gordon, Jennifer Kantor, Rose Twible, Lauren E. Marshall, Stephanie McGarry, Sam Rossi, Laura Bessette, Benoit Baron, Christian Apte, Simon Banfield, Jillian F. Warren, Lesley A. Nat Commun Article The acidification of water in mining areas is a global environmental issue primarily catalyzed by sulfur-oxidizing bacteria (SOB). Little is known about microbial sulfur cycling in circumneutral pH mine tailing impoundment waters. Here we investigate biological sulfur oxidation over four years in a mine tailings impoundment water cap, integrating aqueous sulfur geochemistry, genome-resolved metagenomics and metatranscriptomics. The microbial community is consistently dominated by neutrophilic, chemolithoautotrophic SOB (relative abundances of ~76% in 2015, ~55% in 2016/2017 and ~60% in 2018). Results reveal two SOB strategies alternately dominate across the four years, influencing acid generation and sulfur speciation. Under oxic conditions, novel Halothiobacillus drive lower pH conditions (as low as 4.3) and lower [S(2)O(3)(2−)] via the complete Sox pathway coupled to O(2). Under anoxic conditions, Thiobacillus spp. dominate in activity, via the incomplete Sox and rDSR pathways coupled to NO(3)(−), resulting in higher [S(2)O(3)(2−)] and no net significant acidity generation. This study provides genomic evidence explaining acidity generation and thiosulfate accumulation patterns in a circumneutral mine tailing impoundment and has significant environmental applications in preventing the discharge of sulfur compounds that can impact downstream environments. These insights illuminate opportunities for in situ biotreatment of reduced sulfur compounds and prediction of acidification events using gene-based monitoring and in situ RNA detection. Nature Publishing Group UK 2023-04-10 /pmc/articles/PMC10086054/ /pubmed/37037821 http://dx.doi.org/10.1038/s41467-023-37426-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Whaley-Martin, Kelly J.
Chen, Lin-Xing
Nelson, Tara Colenbrander
Gordon, Jennifer
Kantor, Rose
Twible, Lauren E.
Marshall, Stephanie
McGarry, Sam
Rossi, Laura
Bessette, Benoit
Baron, Christian
Apte, Simon
Banfield, Jillian F.
Warren, Lesley A.
O(2) partitioning of sulfur oxidizing bacteria drives acidity and thiosulfate distributions in mining waters
title O(2) partitioning of sulfur oxidizing bacteria drives acidity and thiosulfate distributions in mining waters
title_full O(2) partitioning of sulfur oxidizing bacteria drives acidity and thiosulfate distributions in mining waters
title_fullStr O(2) partitioning of sulfur oxidizing bacteria drives acidity and thiosulfate distributions in mining waters
title_full_unstemmed O(2) partitioning of sulfur oxidizing bacteria drives acidity and thiosulfate distributions in mining waters
title_short O(2) partitioning of sulfur oxidizing bacteria drives acidity and thiosulfate distributions in mining waters
title_sort o(2) partitioning of sulfur oxidizing bacteria drives acidity and thiosulfate distributions in mining waters
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10086054/
https://www.ncbi.nlm.nih.gov/pubmed/37037821
http://dx.doi.org/10.1038/s41467-023-37426-8
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