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Programming bacteria for multiplexed DNA detection

DNA is a universal and programmable signal of living organisms. Here we develop cell-based DNA sensors by engineering the naturally competent bacterium Bacillus subtilis (B. subtilis) to detect specific DNA sequences in the environment. The DNA sensor strains can identify diverse bacterial species i...

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Autores principales: Cheng, Yu-Yu, Chen, Zhengyi, Cao, Xinyun, Ross, Tyler D., Falbel, Tanya G., Burton, Briana M., Venturelli, Ophelia S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10086068/
https://www.ncbi.nlm.nih.gov/pubmed/37037805
http://dx.doi.org/10.1038/s41467-023-37582-x
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author Cheng, Yu-Yu
Chen, Zhengyi
Cao, Xinyun
Ross, Tyler D.
Falbel, Tanya G.
Burton, Briana M.
Venturelli, Ophelia S.
author_facet Cheng, Yu-Yu
Chen, Zhengyi
Cao, Xinyun
Ross, Tyler D.
Falbel, Tanya G.
Burton, Briana M.
Venturelli, Ophelia S.
author_sort Cheng, Yu-Yu
collection PubMed
description DNA is a universal and programmable signal of living organisms. Here we develop cell-based DNA sensors by engineering the naturally competent bacterium Bacillus subtilis (B. subtilis) to detect specific DNA sequences in the environment. The DNA sensor strains can identify diverse bacterial species including major human pathogens with high specificity. Multiplexed detection of genomic DNA from different species in complex samples can be achieved by coupling the sensing mechanism to orthogonal fluorescent reporters. We also demonstrate that the DNA sensors can detect the presence of species in the complex samples without requiring DNA extraction. The modularity of the living cell-based DNA-sensing mechanism and simple detection procedure could enable programmable DNA sensing for a wide range of applications.
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spelling pubmed-100860682023-04-12 Programming bacteria for multiplexed DNA detection Cheng, Yu-Yu Chen, Zhengyi Cao, Xinyun Ross, Tyler D. Falbel, Tanya G. Burton, Briana M. Venturelli, Ophelia S. Nat Commun Article DNA is a universal and programmable signal of living organisms. Here we develop cell-based DNA sensors by engineering the naturally competent bacterium Bacillus subtilis (B. subtilis) to detect specific DNA sequences in the environment. The DNA sensor strains can identify diverse bacterial species including major human pathogens with high specificity. Multiplexed detection of genomic DNA from different species in complex samples can be achieved by coupling the sensing mechanism to orthogonal fluorescent reporters. We also demonstrate that the DNA sensors can detect the presence of species in the complex samples without requiring DNA extraction. The modularity of the living cell-based DNA-sensing mechanism and simple detection procedure could enable programmable DNA sensing for a wide range of applications. Nature Publishing Group UK 2023-04-10 /pmc/articles/PMC10086068/ /pubmed/37037805 http://dx.doi.org/10.1038/s41467-023-37582-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Cheng, Yu-Yu
Chen, Zhengyi
Cao, Xinyun
Ross, Tyler D.
Falbel, Tanya G.
Burton, Briana M.
Venturelli, Ophelia S.
Programming bacteria for multiplexed DNA detection
title Programming bacteria for multiplexed DNA detection
title_full Programming bacteria for multiplexed DNA detection
title_fullStr Programming bacteria for multiplexed DNA detection
title_full_unstemmed Programming bacteria for multiplexed DNA detection
title_short Programming bacteria for multiplexed DNA detection
title_sort programming bacteria for multiplexed dna detection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10086068/
https://www.ncbi.nlm.nih.gov/pubmed/37037805
http://dx.doi.org/10.1038/s41467-023-37582-x
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