A recombinant porcine reproductive and respiratory syndrome virus type 2 field strain derived from two PRRSV-2-modified live virus vaccines

A porcine reproductive and respiratory syndrome virus (PRRSV) type 2 (PRRSV-2) isolate was obtained from lung samples collected from a 4.5-month-old pig at a wean-to-finish site in Indiana, USA, although no gross or microscopic lesions suggestive of PRRSV infection were observed in the lung tissue....

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Autores principales: Trevisan, Giovani, Magstadt, Drew, Woods, Amy, Sparks, Joel, Zeller, Michael, Li, Ganwu, Krueger, Karen M., Saxena, Anugrah, Zhang, Jianqiang, Gauger, Phillip C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Veterinary Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10086154/
https://www.ncbi.nlm.nih.gov/pubmed/37056231
http://dx.doi.org/10.3389/fvets.2023.1149293
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author Trevisan, Giovani
Magstadt, Drew
Woods, Amy
Sparks, Joel
Zeller, Michael
Li, Ganwu
Krueger, Karen M.
Saxena, Anugrah
Zhang, Jianqiang
Gauger, Phillip C.
author_facet Trevisan, Giovani
Magstadt, Drew
Woods, Amy
Sparks, Joel
Zeller, Michael
Li, Ganwu
Krueger, Karen M.
Saxena, Anugrah
Zhang, Jianqiang
Gauger, Phillip C.
author_sort Trevisan, Giovani
collection PubMed
description A porcine reproductive and respiratory syndrome virus (PRRSV) type 2 (PRRSV-2) isolate was obtained from lung samples collected from a 4.5-month-old pig at a wean-to-finish site in Indiana, USA, although no gross or microscopic lesions suggestive of PRRSV infection were observed in the lung tissue. Phylogenetic and molecular evolutionary analyses based on the obtained virus sequences indicated that PRRSV USA/IN105404/2021 was a natural recombinant isolate from Ingelvac PRRS(®) MLV and Prevacent(®) PRRS, which are PRRSV-2-modified live virus vaccines commercially available in the United States. This study is the first to report the detection of a PRRSV-2 recombinant strain consisting entirely of two modified live virus vaccine strains under field conditions. Based on clinical data and the absence of lung lesions, this PRRSV-2 recombinant strain was not virulent in swine, although its pathogenicity needs to be confirmed by clinical trials.
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spelling pubmed-100861542023-04-12 A recombinant porcine reproductive and respiratory syndrome virus type 2 field strain derived from two PRRSV-2-modified live virus vaccines Trevisan, Giovani Magstadt, Drew Woods, Amy Sparks, Joel Zeller, Michael Li, Ganwu Krueger, Karen M. Saxena, Anugrah Zhang, Jianqiang Gauger, Phillip C. Front Vet Sci Veterinary Science A porcine reproductive and respiratory syndrome virus (PRRSV) type 2 (PRRSV-2) isolate was obtained from lung samples collected from a 4.5-month-old pig at a wean-to-finish site in Indiana, USA, although no gross or microscopic lesions suggestive of PRRSV infection were observed in the lung tissue. Phylogenetic and molecular evolutionary analyses based on the obtained virus sequences indicated that PRRSV USA/IN105404/2021 was a natural recombinant isolate from Ingelvac PRRS(®) MLV and Prevacent(®) PRRS, which are PRRSV-2-modified live virus vaccines commercially available in the United States. This study is the first to report the detection of a PRRSV-2 recombinant strain consisting entirely of two modified live virus vaccine strains under field conditions. Based on clinical data and the absence of lung lesions, this PRRSV-2 recombinant strain was not virulent in swine, although its pathogenicity needs to be confirmed by clinical trials. Frontiers Media S.A. 2023-03-28 /pmc/articles/PMC10086154/ /pubmed/37056231 http://dx.doi.org/10.3389/fvets.2023.1149293 Text en Copyright © 2023 Trevisan, Magstadt, Woods, Sparks, Zeller, Li, Krueger, Saxena, Zhang and Gauger. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Trevisan, Giovani
Magstadt, Drew
Woods, Amy
Sparks, Joel
Zeller, Michael
Li, Ganwu
Krueger, Karen M.
Saxena, Anugrah
Zhang, Jianqiang
Gauger, Phillip C.
A recombinant porcine reproductive and respiratory syndrome virus type 2 field strain derived from two PRRSV-2-modified live virus vaccines
title A recombinant porcine reproductive and respiratory syndrome virus type 2 field strain derived from two PRRSV-2-modified live virus vaccines
title_full A recombinant porcine reproductive and respiratory syndrome virus type 2 field strain derived from two PRRSV-2-modified live virus vaccines
title_fullStr A recombinant porcine reproductive and respiratory syndrome virus type 2 field strain derived from two PRRSV-2-modified live virus vaccines
title_full_unstemmed A recombinant porcine reproductive and respiratory syndrome virus type 2 field strain derived from two PRRSV-2-modified live virus vaccines
title_short A recombinant porcine reproductive and respiratory syndrome virus type 2 field strain derived from two PRRSV-2-modified live virus vaccines
title_sort recombinant porcine reproductive and respiratory syndrome virus type 2 field strain derived from two prrsv-2-modified live virus vaccines
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10086154/
https://www.ncbi.nlm.nih.gov/pubmed/37056231
http://dx.doi.org/10.3389/fvets.2023.1149293
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