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Tonsillar transcriptional profiles in atopic and non‐atopic subjects
BACKGROUND: Emerging research suggests that local lymphatic tissue such as tonsils have important role in regulating the immune responses. However, allergen sensitization‐induced alterations in transcriptome of tonsils are not known. OBJECTIVES: To examine the key differences in tonsillar gene expre...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10087516/ https://www.ncbi.nlm.nih.gov/pubmed/35899482 http://dx.doi.org/10.1111/all.15458 |
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author | Hanif, Tanzeela Ivaska, Lotta E. Ahmad, Freed Tan, Ge Mikola, Emilia Puhakka, Tuomo Palomares, Oscar Akdis, Cezmi A. Toppila‐Salmi, Sanna Jartti, Tuomas |
author_facet | Hanif, Tanzeela Ivaska, Lotta E. Ahmad, Freed Tan, Ge Mikola, Emilia Puhakka, Tuomo Palomares, Oscar Akdis, Cezmi A. Toppila‐Salmi, Sanna Jartti, Tuomas |
author_sort | Hanif, Tanzeela |
collection | PubMed |
description | BACKGROUND: Emerging research suggests that local lymphatic tissue such as tonsils have important role in regulating the immune responses. However, allergen sensitization‐induced alterations in transcriptome of tonsils are not known. OBJECTIVES: To examine the key differences in tonsillar gene expression between atopic and non‐atopic subjects and further by type of sensitization. METHODS: RNA‐sequencing was performed on 52 tonsillar samples from atopic and non‐atopic tonsillectomy patients. Sensitization to common food‐ and aero‐allergen was defined by allergen specific IgE. Following groups were studied: (1) aero‐ and food‐allergen sensitized (AS+FS) versus non‐sensitized (NS), (2) aeroallergen‐sensitized (AS) versus food‐allergen sensitized (FS), (3) AS versus NS, (4) FS versus NS. Bioinformatics analysis was done using DESeq2(v3.10.2), WGCNA and GATK pipeline in R software (v3.3.1). Protein–protein interaction network was made from String database. RESULTS: We studied 13 aeroallergen‐sensitized, 6 food‐allergen sensitized, 4 both food‐and aero‐allergen‐sensitized and 29 non‐sensitized tonsillectomy patients. Overall, 697 unique differentially expressed genes (DEGs) were detected in all sensitized subgroups including chemokines (CXCL2, CXCL8, CXCL10, CXCL11), IL‐20RA, MUC1 and MUC20. When comparing different groups, the gene expression profiles overlapped except the AS versus FS group comparison, suggesting significantly different gene expression between the two sensitization subgroups. Furthermore, aeroallergen‐sensitized subjects had more prominent immune responses compared with non‐sensitized and food‐allergen sensitized subjects including gene expression for IL‐17 pathway and Toll‐like receptor signalling pathway. CONCLUSION: Allergic sensitization is associated with extensive tonsillar transcriptomic alterations and changes in immune related genes and pathways. Distinct differences were found between aero‐allergen and food‐allergen sensitization. |
format | Online Article Text |
id | pubmed-10087516 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100875162023-04-12 Tonsillar transcriptional profiles in atopic and non‐atopic subjects Hanif, Tanzeela Ivaska, Lotta E. Ahmad, Freed Tan, Ge Mikola, Emilia Puhakka, Tuomo Palomares, Oscar Akdis, Cezmi A. Toppila‐Salmi, Sanna Jartti, Tuomas Allergy ORIGINAL ARTICLES BACKGROUND: Emerging research suggests that local lymphatic tissue such as tonsils have important role in regulating the immune responses. However, allergen sensitization‐induced alterations in transcriptome of tonsils are not known. OBJECTIVES: To examine the key differences in tonsillar gene expression between atopic and non‐atopic subjects and further by type of sensitization. METHODS: RNA‐sequencing was performed on 52 tonsillar samples from atopic and non‐atopic tonsillectomy patients. Sensitization to common food‐ and aero‐allergen was defined by allergen specific IgE. Following groups were studied: (1) aero‐ and food‐allergen sensitized (AS+FS) versus non‐sensitized (NS), (2) aeroallergen‐sensitized (AS) versus food‐allergen sensitized (FS), (3) AS versus NS, (4) FS versus NS. Bioinformatics analysis was done using DESeq2(v3.10.2), WGCNA and GATK pipeline in R software (v3.3.1). Protein–protein interaction network was made from String database. RESULTS: We studied 13 aeroallergen‐sensitized, 6 food‐allergen sensitized, 4 both food‐and aero‐allergen‐sensitized and 29 non‐sensitized tonsillectomy patients. Overall, 697 unique differentially expressed genes (DEGs) were detected in all sensitized subgroups including chemokines (CXCL2, CXCL8, CXCL10, CXCL11), IL‐20RA, MUC1 and MUC20. When comparing different groups, the gene expression profiles overlapped except the AS versus FS group comparison, suggesting significantly different gene expression between the two sensitization subgroups. Furthermore, aeroallergen‐sensitized subjects had more prominent immune responses compared with non‐sensitized and food‐allergen sensitized subjects including gene expression for IL‐17 pathway and Toll‐like receptor signalling pathway. CONCLUSION: Allergic sensitization is associated with extensive tonsillar transcriptomic alterations and changes in immune related genes and pathways. Distinct differences were found between aero‐allergen and food‐allergen sensitization. John Wiley and Sons Inc. 2022-08-10 2023-02 /pmc/articles/PMC10087516/ /pubmed/35899482 http://dx.doi.org/10.1111/all.15458 Text en © 2022 The Authors. Allergy published by European Academy of Allergy and Clinical Immunology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | ORIGINAL ARTICLES Hanif, Tanzeela Ivaska, Lotta E. Ahmad, Freed Tan, Ge Mikola, Emilia Puhakka, Tuomo Palomares, Oscar Akdis, Cezmi A. Toppila‐Salmi, Sanna Jartti, Tuomas Tonsillar transcriptional profiles in atopic and non‐atopic subjects |
title | Tonsillar transcriptional profiles in atopic and non‐atopic subjects |
title_full | Tonsillar transcriptional profiles in atopic and non‐atopic subjects |
title_fullStr | Tonsillar transcriptional profiles in atopic and non‐atopic subjects |
title_full_unstemmed | Tonsillar transcriptional profiles in atopic and non‐atopic subjects |
title_short | Tonsillar transcriptional profiles in atopic and non‐atopic subjects |
title_sort | tonsillar transcriptional profiles in atopic and non‐atopic subjects |
topic | ORIGINAL ARTICLES |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10087516/ https://www.ncbi.nlm.nih.gov/pubmed/35899482 http://dx.doi.org/10.1111/all.15458 |
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