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Wide‐ranging migration and destination of early olfactory placode‐derived neurons in chick embryos

Cell migration from the olfactory placode (OP) is a well‐known phenomenon wherein various cell types, such as gonadotropin‐releasing hormone (GnRH)‐producing neurons, migrate toward the telencephalon (TEL) during early embryonic development. However, the spatial relationship between early migratory...

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Autores principales: Miyakawa, Momoko, Murakmai, Shizuko, Uchiyama, Yasuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10087695/
https://www.ncbi.nlm.nih.gov/pubmed/36104941
http://dx.doi.org/10.1002/ar.25080
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author Miyakawa, Momoko
Murakmai, Shizuko
Uchiyama, Yasuo
author_facet Miyakawa, Momoko
Murakmai, Shizuko
Uchiyama, Yasuo
author_sort Miyakawa, Momoko
collection PubMed
description Cell migration from the olfactory placode (OP) is a well‐known phenomenon wherein various cell types, such as gonadotropin‐releasing hormone (GnRH)‐producing neurons, migrate toward the telencephalon (TEL) during early embryonic development. However, the spatial relationship between early migratory cells and the forebrain is unclear. We examined the early development of whole‐mount chick embryos to observe the three‐dimensional spatial relationship among OP‐derived migratory neurons, olfactory nerve (ON), and TEL. Migratory neurons that express highly polysialylated neural cell adhesion molecule (PSA‐NCAM) emerge from the OP and spread over a relatively wide TEL area at the Hamburger and Hamilton (HH) stage 17. Most migratory neurons form a cellular cord between the olfactory pit and rostral TEL within HH18–20. The earliest axons from the olfactory epithelium (OE) were detected along this neuronal cord using DiI‐labeling at HH21. Furthermore, a few PSA‐NCAM‐positive neurons were dispersed around the cellular cord and over the lateral TEL at HH18. A long cellular cord branch extending to the lateral TEL was transiently observed within HH18–24. These results suggest a novel migratory route of OP‐derived neurons during the early developmental stages. Following GFP vector introduction into the OP of HH13–15 embryos, labeled neurons were detected around and within the lateral TEL at HH23 and HH27. At HH36, labeled cells were observed in the rostral‐lateral TEL, including the olfactory bulb (OB) region. GFP‐labeled and calretinin‐positive neurons were detected in the OB, suggesting that early OP‐derived neurons enter the forebrain and function as interneurons in the OB.
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spelling pubmed-100876952023-04-12 Wide‐ranging migration and destination of early olfactory placode‐derived neurons in chick embryos Miyakawa, Momoko Murakmai, Shizuko Uchiyama, Yasuo Anat Rec (Hoboken) Research Articles Cell migration from the olfactory placode (OP) is a well‐known phenomenon wherein various cell types, such as gonadotropin‐releasing hormone (GnRH)‐producing neurons, migrate toward the telencephalon (TEL) during early embryonic development. However, the spatial relationship between early migratory cells and the forebrain is unclear. We examined the early development of whole‐mount chick embryos to observe the three‐dimensional spatial relationship among OP‐derived migratory neurons, olfactory nerve (ON), and TEL. Migratory neurons that express highly polysialylated neural cell adhesion molecule (PSA‐NCAM) emerge from the OP and spread over a relatively wide TEL area at the Hamburger and Hamilton (HH) stage 17. Most migratory neurons form a cellular cord between the olfactory pit and rostral TEL within HH18–20. The earliest axons from the olfactory epithelium (OE) were detected along this neuronal cord using DiI‐labeling at HH21. Furthermore, a few PSA‐NCAM‐positive neurons were dispersed around the cellular cord and over the lateral TEL at HH18. A long cellular cord branch extending to the lateral TEL was transiently observed within HH18–24. These results suggest a novel migratory route of OP‐derived neurons during the early developmental stages. Following GFP vector introduction into the OP of HH13–15 embryos, labeled neurons were detected around and within the lateral TEL at HH23 and HH27. At HH36, labeled cells were observed in the rostral‐lateral TEL, including the olfactory bulb (OB) region. GFP‐labeled and calretinin‐positive neurons were detected in the OB, suggesting that early OP‐derived neurons enter the forebrain and function as interneurons in the OB. John Wiley & Sons, Inc. 2022-09-22 2023-02 /pmc/articles/PMC10087695/ /pubmed/36104941 http://dx.doi.org/10.1002/ar.25080 Text en © 2022 The Authors. The Anatomical Record published by Wiley Periodicals LLC on behalf of American Association for Anatomy. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Miyakawa, Momoko
Murakmai, Shizuko
Uchiyama, Yasuo
Wide‐ranging migration and destination of early olfactory placode‐derived neurons in chick embryos
title Wide‐ranging migration and destination of early olfactory placode‐derived neurons in chick embryos
title_full Wide‐ranging migration and destination of early olfactory placode‐derived neurons in chick embryos
title_fullStr Wide‐ranging migration and destination of early olfactory placode‐derived neurons in chick embryos
title_full_unstemmed Wide‐ranging migration and destination of early olfactory placode‐derived neurons in chick embryos
title_short Wide‐ranging migration and destination of early olfactory placode‐derived neurons in chick embryos
title_sort wide‐ranging migration and destination of early olfactory placode‐derived neurons in chick embryos
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10087695/
https://www.ncbi.nlm.nih.gov/pubmed/36104941
http://dx.doi.org/10.1002/ar.25080
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