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Effect of old age on the subpopulations of enteric glial cells in human descending colon

Old age is associated with a higher incidence of lower bowel conditions such as constipation. Recent evidence suggest that colonic motility may be influenced by enteric glial cells (EGCs). Little is known about the effect of aging on the subpopulation of EGCs in the human colon. We assessed and comp...

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Autores principales: Baidoo, Nicholas, Sanger, Gareth J., Belai, Abi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10087700/
https://www.ncbi.nlm.nih.gov/pubmed/36128665
http://dx.doi.org/10.1002/glia.24272
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author Baidoo, Nicholas
Sanger, Gareth J.
Belai, Abi
author_facet Baidoo, Nicholas
Sanger, Gareth J.
Belai, Abi
author_sort Baidoo, Nicholas
collection PubMed
description Old age is associated with a higher incidence of lower bowel conditions such as constipation. Recent evidence suggest that colonic motility may be influenced by enteric glial cells (EGCs). Little is known about the effect of aging on the subpopulation of EGCs in the human colon. We assessed and compared the pattern of distribution of EGCs in adult and elderly human colon. Human descending colon were obtained from 23 cancer patients comprising of adults (23–63 years; 6 male, 7 female) and elderly (66–81 year; 6 male, 4 female). Specimens were serially‐sectioned and immunolabeled with anti‐Sox‐10, anti‐S100 and anti‐GFAP for morphometric analysis. Standardized procedures were utilized to ensure unbiased counting and densitometric evaluation of EGCs. The number of Sox‐10 immunoreactive (IR) EGCs were unaltered with age in both the myenteric plexus (MP) (respectively, in adult and elderly patients, 1939 ± 82 and 1760 ± 44/mm length; p > .05) and submucosal plexus; there were no apparent differences between adult males and females. The density of S100‐IR EGCs declined among the elderly in the circular muscle and within the MP per ganglionic area. In the adult colon, there were more S100‐IR EGCs distributed in the circular muscle per unit area than the Taenia coli. There was little or no GFAP‐IR EGCs in both adult and elderly colon. We concluded that aging of the human descending colon does not result in a loss of Sox‐10‐IR EGCs in the MP and SMP but reduces S100‐IR EGCs density within the musculature. This alteration in myenteric EGCs density with age may contribute to colonic dysfunction.
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spelling pubmed-100877002023-04-12 Effect of old age on the subpopulations of enteric glial cells in human descending colon Baidoo, Nicholas Sanger, Gareth J. Belai, Abi Glia Research Articles Old age is associated with a higher incidence of lower bowel conditions such as constipation. Recent evidence suggest that colonic motility may be influenced by enteric glial cells (EGCs). Little is known about the effect of aging on the subpopulation of EGCs in the human colon. We assessed and compared the pattern of distribution of EGCs in adult and elderly human colon. Human descending colon were obtained from 23 cancer patients comprising of adults (23–63 years; 6 male, 7 female) and elderly (66–81 year; 6 male, 4 female). Specimens were serially‐sectioned and immunolabeled with anti‐Sox‐10, anti‐S100 and anti‐GFAP for morphometric analysis. Standardized procedures were utilized to ensure unbiased counting and densitometric evaluation of EGCs. The number of Sox‐10 immunoreactive (IR) EGCs were unaltered with age in both the myenteric plexus (MP) (respectively, in adult and elderly patients, 1939 ± 82 and 1760 ± 44/mm length; p > .05) and submucosal plexus; there were no apparent differences between adult males and females. The density of S100‐IR EGCs declined among the elderly in the circular muscle and within the MP per ganglionic area. In the adult colon, there were more S100‐IR EGCs distributed in the circular muscle per unit area than the Taenia coli. There was little or no GFAP‐IR EGCs in both adult and elderly colon. We concluded that aging of the human descending colon does not result in a loss of Sox‐10‐IR EGCs in the MP and SMP but reduces S100‐IR EGCs density within the musculature. This alteration in myenteric EGCs density with age may contribute to colonic dysfunction. John Wiley & Sons, Inc. 2022-09-20 2023-02 /pmc/articles/PMC10087700/ /pubmed/36128665 http://dx.doi.org/10.1002/glia.24272 Text en © 2022 The Authors. GLIA published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Baidoo, Nicholas
Sanger, Gareth J.
Belai, Abi
Effect of old age on the subpopulations of enteric glial cells in human descending colon
title Effect of old age on the subpopulations of enteric glial cells in human descending colon
title_full Effect of old age on the subpopulations of enteric glial cells in human descending colon
title_fullStr Effect of old age on the subpopulations of enteric glial cells in human descending colon
title_full_unstemmed Effect of old age on the subpopulations of enteric glial cells in human descending colon
title_short Effect of old age on the subpopulations of enteric glial cells in human descending colon
title_sort effect of old age on the subpopulations of enteric glial cells in human descending colon
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10087700/
https://www.ncbi.nlm.nih.gov/pubmed/36128665
http://dx.doi.org/10.1002/glia.24272
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