Cargando…

Resonances at Fundamental and Harmonic Frequencies for Selective Imaging of Sine‐Wave Illuminated Reversibly Photoactivatable Labels

We introduce HIGHLIGHT as a simple and general strategy to selectively image a reversibly photoactivatable fluorescent label associated with a given kinetics. The label is submitted to sine‐wave illumination of large amplitude, which generates oscillations of its concentration and fluorescence at hi...

Descripción completa

Detalles Bibliográficos
Autores principales: Pellissier‐Tanon, Agnès, Chouket, Raja, Zhang, Ruikang, Lahlou, Aliénor, Espagne, Agathe, Lemarchand, Annie, Croquette, Vincent, Jullien, Ludovic, Le Saux, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10087976/
https://www.ncbi.nlm.nih.gov/pubmed/35976176
http://dx.doi.org/10.1002/cphc.202200295
_version_ 1785022471396655104
author Pellissier‐Tanon, Agnès
Chouket, Raja
Zhang, Ruikang
Lahlou, Aliénor
Espagne, Agathe
Lemarchand, Annie
Croquette, Vincent
Jullien, Ludovic
Le Saux, Thomas
author_facet Pellissier‐Tanon, Agnès
Chouket, Raja
Zhang, Ruikang
Lahlou, Aliénor
Espagne, Agathe
Lemarchand, Annie
Croquette, Vincent
Jullien, Ludovic
Le Saux, Thomas
author_sort Pellissier‐Tanon, Agnès
collection PubMed
description We introduce HIGHLIGHT as a simple and general strategy to selectively image a reversibly photoactivatable fluorescent label associated with a given kinetics. The label is submitted to sine‐wave illumination of large amplitude, which generates oscillations of its concentration and fluorescence at higher harmonic frequencies. For singularizing a label, HIGHLIGHT uses specific frequencies and mean light intensities associated with resonances of the amplitudes of concentration and fluorescence oscillations at harmonic frequencies. Several non‐redundant resonant observables are simultaneously retrieved from a single experiment with phase‐sensitive detection. HIGHLIGHT is used for selective imaging of four spectrally similar fluorescent proteins that had not been discriminated so far. Moreover, labels out of targeted locations can be discarded in an inhomogeneous spatial profile of illumination. HIGHLIGHT opens roads for simplified optical setups at reduced cost and easier maintenance.
format Online
Article
Text
id pubmed-10087976
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-100879762023-04-12 Resonances at Fundamental and Harmonic Frequencies for Selective Imaging of Sine‐Wave Illuminated Reversibly Photoactivatable Labels Pellissier‐Tanon, Agnès Chouket, Raja Zhang, Ruikang Lahlou, Aliénor Espagne, Agathe Lemarchand, Annie Croquette, Vincent Jullien, Ludovic Le Saux, Thomas Chemphyschem Research Articles We introduce HIGHLIGHT as a simple and general strategy to selectively image a reversibly photoactivatable fluorescent label associated with a given kinetics. The label is submitted to sine‐wave illumination of large amplitude, which generates oscillations of its concentration and fluorescence at higher harmonic frequencies. For singularizing a label, HIGHLIGHT uses specific frequencies and mean light intensities associated with resonances of the amplitudes of concentration and fluorescence oscillations at harmonic frequencies. Several non‐redundant resonant observables are simultaneously retrieved from a single experiment with phase‐sensitive detection. HIGHLIGHT is used for selective imaging of four spectrally similar fluorescent proteins that had not been discriminated so far. Moreover, labels out of targeted locations can be discarded in an inhomogeneous spatial profile of illumination. HIGHLIGHT opens roads for simplified optical setups at reduced cost and easier maintenance. John Wiley and Sons Inc. 2022-09-21 2022-12-05 /pmc/articles/PMC10087976/ /pubmed/35976176 http://dx.doi.org/10.1002/cphc.202200295 Text en © 2022 The Authors. ChemPhysChem published by Wiley-VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Pellissier‐Tanon, Agnès
Chouket, Raja
Zhang, Ruikang
Lahlou, Aliénor
Espagne, Agathe
Lemarchand, Annie
Croquette, Vincent
Jullien, Ludovic
Le Saux, Thomas
Resonances at Fundamental and Harmonic Frequencies for Selective Imaging of Sine‐Wave Illuminated Reversibly Photoactivatable Labels
title Resonances at Fundamental and Harmonic Frequencies for Selective Imaging of Sine‐Wave Illuminated Reversibly Photoactivatable Labels
title_full Resonances at Fundamental and Harmonic Frequencies for Selective Imaging of Sine‐Wave Illuminated Reversibly Photoactivatable Labels
title_fullStr Resonances at Fundamental and Harmonic Frequencies for Selective Imaging of Sine‐Wave Illuminated Reversibly Photoactivatable Labels
title_full_unstemmed Resonances at Fundamental and Harmonic Frequencies for Selective Imaging of Sine‐Wave Illuminated Reversibly Photoactivatable Labels
title_short Resonances at Fundamental and Harmonic Frequencies for Selective Imaging of Sine‐Wave Illuminated Reversibly Photoactivatable Labels
title_sort resonances at fundamental and harmonic frequencies for selective imaging of sine‐wave illuminated reversibly photoactivatable labels
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10087976/
https://www.ncbi.nlm.nih.gov/pubmed/35976176
http://dx.doi.org/10.1002/cphc.202200295
work_keys_str_mv AT pellissiertanonagnes resonancesatfundamentalandharmonicfrequenciesforselectiveimagingofsinewaveilluminatedreversiblyphotoactivatablelabels
AT chouketraja resonancesatfundamentalandharmonicfrequenciesforselectiveimagingofsinewaveilluminatedreversiblyphotoactivatablelabels
AT zhangruikang resonancesatfundamentalandharmonicfrequenciesforselectiveimagingofsinewaveilluminatedreversiblyphotoactivatablelabels
AT lahloualienor resonancesatfundamentalandharmonicfrequenciesforselectiveimagingofsinewaveilluminatedreversiblyphotoactivatablelabels
AT espagneagathe resonancesatfundamentalandharmonicfrequenciesforselectiveimagingofsinewaveilluminatedreversiblyphotoactivatablelabels
AT lemarchandannie resonancesatfundamentalandharmonicfrequenciesforselectiveimagingofsinewaveilluminatedreversiblyphotoactivatablelabels
AT croquettevincent resonancesatfundamentalandharmonicfrequenciesforselectiveimagingofsinewaveilluminatedreversiblyphotoactivatablelabels
AT jullienludovic resonancesatfundamentalandharmonicfrequenciesforselectiveimagingofsinewaveilluminatedreversiblyphotoactivatablelabels
AT lesauxthomas resonancesatfundamentalandharmonicfrequenciesforselectiveimagingofsinewaveilluminatedreversiblyphotoactivatablelabels