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Inhibition of STAT3 signaling as critical molecular event in HUC-MSCs suppressed Glioblastoma Cells

Objective: We investigated the effect of human umbilical cord mesenchymal stem cells (HUC-MSCs) supernatants on proliferation, migration, invasion, and apoptosis in glioblastoma (GBM) cell lines RG-2, U251, U87-MG, and LN-428, as well as their apoptosis and autophagy-mediated through IL-6/JAK2/STAT3...

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Autores principales: Wang, Mingming, Zhang, Yufu, Liu, Min, Jia, Yuna, He, Jing, Xu, Xiangrong, Shi, Haiyan, Zhang, Yunqing, Zhang, Jing, Liu, Yusi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10088538/
https://www.ncbi.nlm.nih.gov/pubmed/37057281
http://dx.doi.org/10.7150/jca.77905
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author Wang, Mingming
Zhang, Yufu
Liu, Min
Jia, Yuna
He, Jing
Xu, Xiangrong
Shi, Haiyan
Zhang, Yunqing
Zhang, Jing
Liu, Yusi
author_facet Wang, Mingming
Zhang, Yufu
Liu, Min
Jia, Yuna
He, Jing
Xu, Xiangrong
Shi, Haiyan
Zhang, Yunqing
Zhang, Jing
Liu, Yusi
author_sort Wang, Mingming
collection PubMed
description Objective: We investigated the effect of human umbilical cord mesenchymal stem cells (HUC-MSCs) supernatants on proliferation, migration, invasion, and apoptosis in glioblastoma (GBM) cell lines RG-2, U251, U87-MG, and LN-428, as well as their apoptosis and autophagy-mediated through IL-6/JAK2/STAT3 signaling pathway to explore the molecular mechanisms. Methods: In this study, RG-2, U251, U87-MG, and LN-428 cells were treated with 9 mg/ml HUC-MSCs supernatants. Their responses to HUC-MSCs supernatants treatment and the status of STAT3 signaling were analyzed by multiple experimental approaches to elucidate the importance of HUC-MSCs supernatants for GBM. Results: The results demonstrated that after treatment with HUC-MSCs supernatants, in vitro proliferation of RG-2, U251, U87-MG, and LN-428 cells were inhibited, and their sustained growth was also blocked. RG-2, U251, and U87-MG cells showed significant S phase accumulation, while LN-428 cells were blocked in G0/G1 phase. Their migratory invasive capacities were inhibited, and their apoptosis and autophagy ratios were increased. These effects were mediated through the IL-6/JAK2/STAT3 and its downstream signaling pathway. Conclusion: Our data showed that HUC-MSCs supernatants had anti-tumor effects on GBM cells. It inhibited the proliferation, migration, and invasion of GBM cells and promoted their apoptosis. Negative regulation of the IL-6/JAK2/STAT3 signaling pathway enhanced apoptosis and autophagy in tumor cells, thereby improving the therapeutic effect on GBM.
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spelling pubmed-100885382023-04-12 Inhibition of STAT3 signaling as critical molecular event in HUC-MSCs suppressed Glioblastoma Cells Wang, Mingming Zhang, Yufu Liu, Min Jia, Yuna He, Jing Xu, Xiangrong Shi, Haiyan Zhang, Yunqing Zhang, Jing Liu, Yusi J Cancer Research Paper Objective: We investigated the effect of human umbilical cord mesenchymal stem cells (HUC-MSCs) supernatants on proliferation, migration, invasion, and apoptosis in glioblastoma (GBM) cell lines RG-2, U251, U87-MG, and LN-428, as well as their apoptosis and autophagy-mediated through IL-6/JAK2/STAT3 signaling pathway to explore the molecular mechanisms. Methods: In this study, RG-2, U251, U87-MG, and LN-428 cells were treated with 9 mg/ml HUC-MSCs supernatants. Their responses to HUC-MSCs supernatants treatment and the status of STAT3 signaling were analyzed by multiple experimental approaches to elucidate the importance of HUC-MSCs supernatants for GBM. Results: The results demonstrated that after treatment with HUC-MSCs supernatants, in vitro proliferation of RG-2, U251, U87-MG, and LN-428 cells were inhibited, and their sustained growth was also blocked. RG-2, U251, and U87-MG cells showed significant S phase accumulation, while LN-428 cells were blocked in G0/G1 phase. Their migratory invasive capacities were inhibited, and their apoptosis and autophagy ratios were increased. These effects were mediated through the IL-6/JAK2/STAT3 and its downstream signaling pathway. Conclusion: Our data showed that HUC-MSCs supernatants had anti-tumor effects on GBM cells. It inhibited the proliferation, migration, and invasion of GBM cells and promoted their apoptosis. Negative regulation of the IL-6/JAK2/STAT3 signaling pathway enhanced apoptosis and autophagy in tumor cells, thereby improving the therapeutic effect on GBM. Ivyspring International Publisher 2023-02-27 /pmc/articles/PMC10088538/ /pubmed/37057281 http://dx.doi.org/10.7150/jca.77905 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Wang, Mingming
Zhang, Yufu
Liu, Min
Jia, Yuna
He, Jing
Xu, Xiangrong
Shi, Haiyan
Zhang, Yunqing
Zhang, Jing
Liu, Yusi
Inhibition of STAT3 signaling as critical molecular event in HUC-MSCs suppressed Glioblastoma Cells
title Inhibition of STAT3 signaling as critical molecular event in HUC-MSCs suppressed Glioblastoma Cells
title_full Inhibition of STAT3 signaling as critical molecular event in HUC-MSCs suppressed Glioblastoma Cells
title_fullStr Inhibition of STAT3 signaling as critical molecular event in HUC-MSCs suppressed Glioblastoma Cells
title_full_unstemmed Inhibition of STAT3 signaling as critical molecular event in HUC-MSCs suppressed Glioblastoma Cells
title_short Inhibition of STAT3 signaling as critical molecular event in HUC-MSCs suppressed Glioblastoma Cells
title_sort inhibition of stat3 signaling as critical molecular event in huc-mscs suppressed glioblastoma cells
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10088538/
https://www.ncbi.nlm.nih.gov/pubmed/37057281
http://dx.doi.org/10.7150/jca.77905
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