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Characterisation of NPFF-expressing neurons in the superficial dorsal horn of the mouse spinal cord
Excitatory interneurons in the superficial dorsal horn (SDH) are heterogeneous, and include a class known as vertical cells, which convey information to lamina I projection neurons. We recently used pro-NPFF antibody to reveal a discrete population of excitatory interneurons that express neuropeptid...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10090074/ https://www.ncbi.nlm.nih.gov/pubmed/37041197 http://dx.doi.org/10.1038/s41598-023-32720-3 |
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author | Quillet, Raphaëlle Dickie, Allen C. Polgár, Erika Gutierrez-Mecinas, Maria Bell, Andrew M. Goffin, Luca Watanabe, Masahiko Todd, Andrew J. |
author_facet | Quillet, Raphaëlle Dickie, Allen C. Polgár, Erika Gutierrez-Mecinas, Maria Bell, Andrew M. Goffin, Luca Watanabe, Masahiko Todd, Andrew J. |
author_sort | Quillet, Raphaëlle |
collection | PubMed |
description | Excitatory interneurons in the superficial dorsal horn (SDH) are heterogeneous, and include a class known as vertical cells, which convey information to lamina I projection neurons. We recently used pro-NPFF antibody to reveal a discrete population of excitatory interneurons that express neuropeptide FF (NPFF). Here, we generated a new mouse line (NPFF(Cre)) in which Cre is knocked into the Npff locus, and used Cre-dependent viruses and reporter mice to characterise NPFF cell properties. Both viral and reporter strategies labelled many cells in the SDH, and captured most pro-NPFF-immunoreactive neurons (75–80%). However, the majority of labelled cells lacked pro-NPFF, and we found considerable overlap with a population of neurons that express the gastrin-releasing peptide receptor (GRPR). Morphological reconstruction revealed that most pro-NPFF-containing neurons were vertical cells, but these differed from GRPR neurons (which are also vertical cells) in having a far higher dendritic spine density. Electrophysiological recording showed that NPFF cells also differed from GRPR cells in having a higher frequency of miniature EPSCs, being more electrically excitable and responding to a NPY Y1 receptor agonist. Together, these findings indicate that there are at least two distinct classes of vertical cells, which may have differing roles in somatosensory processing. |
format | Online Article Text |
id | pubmed-10090074 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-100900742023-04-13 Characterisation of NPFF-expressing neurons in the superficial dorsal horn of the mouse spinal cord Quillet, Raphaëlle Dickie, Allen C. Polgár, Erika Gutierrez-Mecinas, Maria Bell, Andrew M. Goffin, Luca Watanabe, Masahiko Todd, Andrew J. Sci Rep Article Excitatory interneurons in the superficial dorsal horn (SDH) are heterogeneous, and include a class known as vertical cells, which convey information to lamina I projection neurons. We recently used pro-NPFF antibody to reveal a discrete population of excitatory interneurons that express neuropeptide FF (NPFF). Here, we generated a new mouse line (NPFF(Cre)) in which Cre is knocked into the Npff locus, and used Cre-dependent viruses and reporter mice to characterise NPFF cell properties. Both viral and reporter strategies labelled many cells in the SDH, and captured most pro-NPFF-immunoreactive neurons (75–80%). However, the majority of labelled cells lacked pro-NPFF, and we found considerable overlap with a population of neurons that express the gastrin-releasing peptide receptor (GRPR). Morphological reconstruction revealed that most pro-NPFF-containing neurons were vertical cells, but these differed from GRPR neurons (which are also vertical cells) in having a far higher dendritic spine density. Electrophysiological recording showed that NPFF cells also differed from GRPR cells in having a higher frequency of miniature EPSCs, being more electrically excitable and responding to a NPY Y1 receptor agonist. Together, these findings indicate that there are at least two distinct classes of vertical cells, which may have differing roles in somatosensory processing. Nature Publishing Group UK 2023-04-11 /pmc/articles/PMC10090074/ /pubmed/37041197 http://dx.doi.org/10.1038/s41598-023-32720-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Quillet, Raphaëlle Dickie, Allen C. Polgár, Erika Gutierrez-Mecinas, Maria Bell, Andrew M. Goffin, Luca Watanabe, Masahiko Todd, Andrew J. Characterisation of NPFF-expressing neurons in the superficial dorsal horn of the mouse spinal cord |
title | Characterisation of NPFF-expressing neurons in the superficial dorsal horn of the mouse spinal cord |
title_full | Characterisation of NPFF-expressing neurons in the superficial dorsal horn of the mouse spinal cord |
title_fullStr | Characterisation of NPFF-expressing neurons in the superficial dorsal horn of the mouse spinal cord |
title_full_unstemmed | Characterisation of NPFF-expressing neurons in the superficial dorsal horn of the mouse spinal cord |
title_short | Characterisation of NPFF-expressing neurons in the superficial dorsal horn of the mouse spinal cord |
title_sort | characterisation of npff-expressing neurons in the superficial dorsal horn of the mouse spinal cord |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10090074/ https://www.ncbi.nlm.nih.gov/pubmed/37041197 http://dx.doi.org/10.1038/s41598-023-32720-3 |
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