Engineered CRISPR-OsCas12f1 and RhCas12f1 with robust activities and expanded target range for genome editing

The type V-F CRISPR-Cas12f system is a strong candidate for therapeutic applications due to the compact size of the Cas12f proteins. In this work, we identify six uncharacterized Cas12f1 proteins with nuclease activity in mammalian cells from assembled bacterial genomes. Among them, OsCas12f1 (433 a...

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Detalles Bibliográficos
Autores principales: Kong, Xiangfeng, Zhang, Hainan, Li, Guoling, Wang, Zikang, Kong, Xuqiang, Wang, Lecong, Xue, Mingxing, Zhang, Weihong, Wang, Yao, Lin, Jiajia, Zhou, Jingxing, Shen, Xiaowen, Wei, Yinghui, Zhong, Na, Bai, Weiya, Yuan, Yuan, Shi, Linyu, Zhou, Yingsi, Yang, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10090079/
https://www.ncbi.nlm.nih.gov/pubmed/37041195
http://dx.doi.org/10.1038/s41467-023-37829-7

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