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Potential inhibitory effects of compounds ZK-PI-5 and ZK-PI-9 on trehalose and chitin metabolism in Spodoptera frugiperda (J. E. Smith)

Introduction: Spodoptera frugiperda is an omnivorous agricultural pest which is great dangerous for grain output. Methods: In order to investigate the effects of potential trehalase inhibitors, ZK-PI-5 and ZK-PI-9, on the growth and development of S. frugiperda, and to identify new avenues for S. fr...

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Autores principales: Zhong, Fan, Yu, Liuhe, Jiang, Xinyi, Chen, Yan, Wang, Sitong, Chao, Lei, Jiang, Zhiyang, He, Biner, Xu, Caidi, Wang, Shigui, Tang, Bin, Duan, Hongxia, Wu, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10090375/
https://www.ncbi.nlm.nih.gov/pubmed/37064912
http://dx.doi.org/10.3389/fphys.2023.1178996
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author Zhong, Fan
Yu, Liuhe
Jiang, Xinyi
Chen, Yan
Wang, Sitong
Chao, Lei
Jiang, Zhiyang
He, Biner
Xu, Caidi
Wang, Shigui
Tang, Bin
Duan, Hongxia
Wu, Yan
author_facet Zhong, Fan
Yu, Liuhe
Jiang, Xinyi
Chen, Yan
Wang, Sitong
Chao, Lei
Jiang, Zhiyang
He, Biner
Xu, Caidi
Wang, Shigui
Tang, Bin
Duan, Hongxia
Wu, Yan
author_sort Zhong, Fan
collection PubMed
description Introduction: Spodoptera frugiperda is an omnivorous agricultural pest which is great dangerous for grain output. Methods: In order to investigate the effects of potential trehalase inhibitors, ZK-PI-5 and ZK-PI-9, on the growth and development of S. frugiperda, and to identify new avenues for S. frugiperda control, we measured the content of the trehalose, glucose, glycogen and chitin, enzyme activity, and gene expression levels in trehalose and chitin metabolism of S. frugiperda. Besides, their growth and development were also observed. Results: The results showed that ZK-PI-9 significantly reduced trehalase activity and ZK-PI-5 significantly reduced membraned-bound trehalase activity. Moreover, ZK-PI-5 inhibited the expression of SfTRE2, SfCHS2, and SfCHT, thus affecting the chitin metabolism. In addition, the mortality of S. frugiperda in pupal stage and eclosion stage increased significantly after treatment with ZK-PI-5 and ZK-PI-9, which affected their development stage and caused death phenotype (abnormal pupation and difficulty in breaking pupa). Discussion: These results have provided a theoretical basis for the application of trehalase inhibitors in the control of agricultural pests to promote future global grain yield.
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spelling pubmed-100903752023-04-13 Potential inhibitory effects of compounds ZK-PI-5 and ZK-PI-9 on trehalose and chitin metabolism in Spodoptera frugiperda (J. E. Smith) Zhong, Fan Yu, Liuhe Jiang, Xinyi Chen, Yan Wang, Sitong Chao, Lei Jiang, Zhiyang He, Biner Xu, Caidi Wang, Shigui Tang, Bin Duan, Hongxia Wu, Yan Front Physiol Physiology Introduction: Spodoptera frugiperda is an omnivorous agricultural pest which is great dangerous for grain output. Methods: In order to investigate the effects of potential trehalase inhibitors, ZK-PI-5 and ZK-PI-9, on the growth and development of S. frugiperda, and to identify new avenues for S. frugiperda control, we measured the content of the trehalose, glucose, glycogen and chitin, enzyme activity, and gene expression levels in trehalose and chitin metabolism of S. frugiperda. Besides, their growth and development were also observed. Results: The results showed that ZK-PI-9 significantly reduced trehalase activity and ZK-PI-5 significantly reduced membraned-bound trehalase activity. Moreover, ZK-PI-5 inhibited the expression of SfTRE2, SfCHS2, and SfCHT, thus affecting the chitin metabolism. In addition, the mortality of S. frugiperda in pupal stage and eclosion stage increased significantly after treatment with ZK-PI-5 and ZK-PI-9, which affected their development stage and caused death phenotype (abnormal pupation and difficulty in breaking pupa). Discussion: These results have provided a theoretical basis for the application of trehalase inhibitors in the control of agricultural pests to promote future global grain yield. Frontiers Media S.A. 2023-03-29 /pmc/articles/PMC10090375/ /pubmed/37064912 http://dx.doi.org/10.3389/fphys.2023.1178996 Text en Copyright © 2023 Zhong, Yu, Jiang, Chen, Wang, Chao, Jiang, He, Xu, Wang, Tang, Duan and Wu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Zhong, Fan
Yu, Liuhe
Jiang, Xinyi
Chen, Yan
Wang, Sitong
Chao, Lei
Jiang, Zhiyang
He, Biner
Xu, Caidi
Wang, Shigui
Tang, Bin
Duan, Hongxia
Wu, Yan
Potential inhibitory effects of compounds ZK-PI-5 and ZK-PI-9 on trehalose and chitin metabolism in Spodoptera frugiperda (J. E. Smith)
title Potential inhibitory effects of compounds ZK-PI-5 and ZK-PI-9 on trehalose and chitin metabolism in Spodoptera frugiperda (J. E. Smith)
title_full Potential inhibitory effects of compounds ZK-PI-5 and ZK-PI-9 on trehalose and chitin metabolism in Spodoptera frugiperda (J. E. Smith)
title_fullStr Potential inhibitory effects of compounds ZK-PI-5 and ZK-PI-9 on trehalose and chitin metabolism in Spodoptera frugiperda (J. E. Smith)
title_full_unstemmed Potential inhibitory effects of compounds ZK-PI-5 and ZK-PI-9 on trehalose and chitin metabolism in Spodoptera frugiperda (J. E. Smith)
title_short Potential inhibitory effects of compounds ZK-PI-5 and ZK-PI-9 on trehalose and chitin metabolism in Spodoptera frugiperda (J. E. Smith)
title_sort potential inhibitory effects of compounds zk-pi-5 and zk-pi-9 on trehalose and chitin metabolism in spodoptera frugiperda (j. e. smith)
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10090375/
https://www.ncbi.nlm.nih.gov/pubmed/37064912
http://dx.doi.org/10.3389/fphys.2023.1178996
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