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Ultrasound-assisted extraction optimization of polyphenols from Boletus bicolor and evaluation of its antioxidant activity

INTRODUCTION: Boletus bicolor (B. bicolor) mushrooms are widely consumed as a valuable medicinal and dietary ingredient in China, but the active ingredients of this mushroom and their extraction methods were not extensively studied. METHODS: In this paper, we propose an optimized ultrasound-assisted...

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Autores principales: Hu, Dong-Bao, Xue, Rui, Zhuang, Xiao-Cui, Zhang, Xin-Sha, Shi, Sheng-Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10090463/
https://www.ncbi.nlm.nih.gov/pubmed/37063317
http://dx.doi.org/10.3389/fnut.2023.1135712
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author Hu, Dong-Bao
Xue, Rui
Zhuang, Xiao-Cui
Zhang, Xin-Sha
Shi, Sheng-Li
author_facet Hu, Dong-Bao
Xue, Rui
Zhuang, Xiao-Cui
Zhang, Xin-Sha
Shi, Sheng-Li
author_sort Hu, Dong-Bao
collection PubMed
description INTRODUCTION: Boletus bicolor (B. bicolor) mushrooms are widely consumed as a valuable medicinal and dietary ingredient in China, but the active ingredients of this mushroom and their extraction methods were not extensively studied. METHODS: In this paper, we propose an optimized ultrasound-assisted extraction (UAE) method to detect natural antioxidant substances in B. bicolor. The antioxidants were quantitatively and quantitatively determined using UPLC-MS, the polyphenols were evaluated based on response surface methodology (RSM), and density functional theory (DFT) calculations were performed. RESULTS: The results showed that the optimal extraction was obtained under the following conditions: ethanol concentration 42%; solvent to solid ratio 34:1 mL/g; ultrasonic time 41 min; and temperature 40°C. The optimized experimental polyphenol value obtained under these conditions was (13.69 ± 0.13) mg/g, consistent with the predicted value of 13.72 mg/g. Eight phenolic compounds in the extract were identiffed by UPLC-MS: syringic acid, chlorogenic acid, gallic acid, rosmarinic acid, protocatechuic acid, catechin, caffeic acid, and quercetin. Chlorogenic acid exhibits the highest HOMO energy (−0.02744 eV) and the lowest energy difference (−0.23450 eV) among the studied compounds, suggesting that the compound might be the strongest antioxidant molecule. Eight phenolic compounds from the B. bicolor signiffcantly inhibited intracellular reactive oxygen species (ROS) generation, reduced oxidative stress damage in H(2)O(2)-induced HepG-2 cells. DISCUSSION: Therefore, it was confirmed that the UAE technique is an efficient, rapid, and simple approach for extracting polyphenols with antioxidant activity from B. bicolor.
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spelling pubmed-100904632023-04-13 Ultrasound-assisted extraction optimization of polyphenols from Boletus bicolor and evaluation of its antioxidant activity Hu, Dong-Bao Xue, Rui Zhuang, Xiao-Cui Zhang, Xin-Sha Shi, Sheng-Li Front Nutr Nutrition INTRODUCTION: Boletus bicolor (B. bicolor) mushrooms are widely consumed as a valuable medicinal and dietary ingredient in China, but the active ingredients of this mushroom and their extraction methods were not extensively studied. METHODS: In this paper, we propose an optimized ultrasound-assisted extraction (UAE) method to detect natural antioxidant substances in B. bicolor. The antioxidants were quantitatively and quantitatively determined using UPLC-MS, the polyphenols were evaluated based on response surface methodology (RSM), and density functional theory (DFT) calculations were performed. RESULTS: The results showed that the optimal extraction was obtained under the following conditions: ethanol concentration 42%; solvent to solid ratio 34:1 mL/g; ultrasonic time 41 min; and temperature 40°C. The optimized experimental polyphenol value obtained under these conditions was (13.69 ± 0.13) mg/g, consistent with the predicted value of 13.72 mg/g. Eight phenolic compounds in the extract were identiffed by UPLC-MS: syringic acid, chlorogenic acid, gallic acid, rosmarinic acid, protocatechuic acid, catechin, caffeic acid, and quercetin. Chlorogenic acid exhibits the highest HOMO energy (−0.02744 eV) and the lowest energy difference (−0.23450 eV) among the studied compounds, suggesting that the compound might be the strongest antioxidant molecule. Eight phenolic compounds from the B. bicolor signiffcantly inhibited intracellular reactive oxygen species (ROS) generation, reduced oxidative stress damage in H(2)O(2)-induced HepG-2 cells. DISCUSSION: Therefore, it was confirmed that the UAE technique is an efficient, rapid, and simple approach for extracting polyphenols with antioxidant activity from B. bicolor. Frontiers Media S.A. 2023-03-29 /pmc/articles/PMC10090463/ /pubmed/37063317 http://dx.doi.org/10.3389/fnut.2023.1135712 Text en Copyright © 2023 Hu, Xue, Zhuang, Zhang and Shi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Hu, Dong-Bao
Xue, Rui
Zhuang, Xiao-Cui
Zhang, Xin-Sha
Shi, Sheng-Li
Ultrasound-assisted extraction optimization of polyphenols from Boletus bicolor and evaluation of its antioxidant activity
title Ultrasound-assisted extraction optimization of polyphenols from Boletus bicolor and evaluation of its antioxidant activity
title_full Ultrasound-assisted extraction optimization of polyphenols from Boletus bicolor and evaluation of its antioxidant activity
title_fullStr Ultrasound-assisted extraction optimization of polyphenols from Boletus bicolor and evaluation of its antioxidant activity
title_full_unstemmed Ultrasound-assisted extraction optimization of polyphenols from Boletus bicolor and evaluation of its antioxidant activity
title_short Ultrasound-assisted extraction optimization of polyphenols from Boletus bicolor and evaluation of its antioxidant activity
title_sort ultrasound-assisted extraction optimization of polyphenols from boletus bicolor and evaluation of its antioxidant activity
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10090463/
https://www.ncbi.nlm.nih.gov/pubmed/37063317
http://dx.doi.org/10.3389/fnut.2023.1135712
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