A novel recombinant serotype 4 fowl adenovirus expressing fiber-2 protein of duck adenovirus 3

Recently, the highly pathogenic serotype 4 fowl adenovirus (FAdV-4) and duck adenovirus 3 (DAdV-3) were outbroken and widespread, causing substantial economic losses to the duck industry. Therefore, there is an urgent need to generate a recombinant genetic engineering vaccine candidate against both...

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Autores principales: Guo, Yiwen, Lin, Yun, Xie, Quan, Zhang, Wenyuan, Xu, Zhenqi, Chao, Yifei, Cao, Xudong, Jiang, Huiru, Li, Han, Li, Tuofan, Wan, Zhimin, Shao, Hongxia, Qin, Aijian, Ye, Jianqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Cellular and Infection Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10090666/
https://www.ncbi.nlm.nih.gov/pubmed/37065194
http://dx.doi.org/10.3389/fcimb.2023.1177866
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author Guo, Yiwen
Lin, Yun
Xie, Quan
Zhang, Wenyuan
Xu, Zhenqi
Chao, Yifei
Cao, Xudong
Jiang, Huiru
Li, Han
Li, Tuofan
Wan, Zhimin
Shao, Hongxia
Qin, Aijian
Ye, Jianqiang
author_facet Guo, Yiwen
Lin, Yun
Xie, Quan
Zhang, Wenyuan
Xu, Zhenqi
Chao, Yifei
Cao, Xudong
Jiang, Huiru
Li, Han
Li, Tuofan
Wan, Zhimin
Shao, Hongxia
Qin, Aijian
Ye, Jianqiang
author_sort Guo, Yiwen
collection PubMed
description Recently, the highly pathogenic serotype 4 fowl adenovirus (FAdV-4) and duck adenovirus 3 (DAdV-3) were outbroken and widespread, causing substantial economic losses to the duck industry. Therefore, there is an urgent need to generate a recombinant genetic engineering vaccine candidate against both FAdV-4 and DAdV-3. In this study, a novel recombinant FAdV-4 expressing the Fiber-2 protein of DAdV-3, designated as rFAdV-4-Fiber-2/DAdV-3, was generated based on CRISPR/Cas9 and Cre-LoxP systems. Indirect immunofluorescence assay (IFA) and western blot (WB) showed that the Fiber-2 protein of DAdV-3 in rFAdV-4-Fiber-2/DAdV-3 was expressed successfully. Moreover, the growth curve revealed that rFAdV-4-Fiber-2/DAdV-3 replicated efficiently in LMH cells and even showed a stronger replication ability compared to the wild type FAdV-4. The generation of the recombinant rFAdV-4-Fiber-2/DAdV-3 provides a potential vaccine candidate against both FAdV-4 and DAdV-3.
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spelling pubmed-100906662023-04-13 A novel recombinant serotype 4 fowl adenovirus expressing fiber-2 protein of duck adenovirus 3 Guo, Yiwen Lin, Yun Xie, Quan Zhang, Wenyuan Xu, Zhenqi Chao, Yifei Cao, Xudong Jiang, Huiru Li, Han Li, Tuofan Wan, Zhimin Shao, Hongxia Qin, Aijian Ye, Jianqiang Front Cell Infect Microbiol Cellular and Infection Microbiology Recently, the highly pathogenic serotype 4 fowl adenovirus (FAdV-4) and duck adenovirus 3 (DAdV-3) were outbroken and widespread, causing substantial economic losses to the duck industry. Therefore, there is an urgent need to generate a recombinant genetic engineering vaccine candidate against both FAdV-4 and DAdV-3. In this study, a novel recombinant FAdV-4 expressing the Fiber-2 protein of DAdV-3, designated as rFAdV-4-Fiber-2/DAdV-3, was generated based on CRISPR/Cas9 and Cre-LoxP systems. Indirect immunofluorescence assay (IFA) and western blot (WB) showed that the Fiber-2 protein of DAdV-3 in rFAdV-4-Fiber-2/DAdV-3 was expressed successfully. Moreover, the growth curve revealed that rFAdV-4-Fiber-2/DAdV-3 replicated efficiently in LMH cells and even showed a stronger replication ability compared to the wild type FAdV-4. The generation of the recombinant rFAdV-4-Fiber-2/DAdV-3 provides a potential vaccine candidate against both FAdV-4 and DAdV-3. Frontiers Media S.A. 2023-03-28 /pmc/articles/PMC10090666/ /pubmed/37065194 http://dx.doi.org/10.3389/fcimb.2023.1177866 Text en Copyright © 2023 Guo, Lin, Xie, Zhang, Xu, Chao, Cao, Jiang, Li, Li, Wan, Shao, Qin and Ye https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Guo, Yiwen
Lin, Yun
Xie, Quan
Zhang, Wenyuan
Xu, Zhenqi
Chao, Yifei
Cao, Xudong
Jiang, Huiru
Li, Han
Li, Tuofan
Wan, Zhimin
Shao, Hongxia
Qin, Aijian
Ye, Jianqiang
A novel recombinant serotype 4 fowl adenovirus expressing fiber-2 protein of duck adenovirus 3
title A novel recombinant serotype 4 fowl adenovirus expressing fiber-2 protein of duck adenovirus 3
title_full A novel recombinant serotype 4 fowl adenovirus expressing fiber-2 protein of duck adenovirus 3
title_fullStr A novel recombinant serotype 4 fowl adenovirus expressing fiber-2 protein of duck adenovirus 3
title_full_unstemmed A novel recombinant serotype 4 fowl adenovirus expressing fiber-2 protein of duck adenovirus 3
title_short A novel recombinant serotype 4 fowl adenovirus expressing fiber-2 protein of duck adenovirus 3
title_sort novel recombinant serotype 4 fowl adenovirus expressing fiber-2 protein of duck adenovirus 3
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10090666/
https://www.ncbi.nlm.nih.gov/pubmed/37065194
http://dx.doi.org/10.3389/fcimb.2023.1177866
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