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Effects of milk-derived bioactive peptide VPP on diarrhea of pre-weaning calves

A well-known milk-derived bioactive tripeptide, VPP (Val-Pro-Pro) has good anti-inflammatory, anti-hypertension, and anti-hydrolysis properties. However, whether VPP can alleviate calf intestinal inflammation is unclear. In this experiment, the effects of VPP on growth, diarrhea incidence, serum bio...

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Autores principales: Zong, Xiaomei, Gao, Ya, Du, Yufeng, Hou, Jinxiu, Yang, Linhai, Xu, Qingbiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10090956/
https://www.ncbi.nlm.nih.gov/pubmed/37065247
http://dx.doi.org/10.3389/fvets.2023.1154197
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author Zong, Xiaomei
Gao, Ya
Du, Yufeng
Hou, Jinxiu
Yang, Linhai
Xu, Qingbiao
author_facet Zong, Xiaomei
Gao, Ya
Du, Yufeng
Hou, Jinxiu
Yang, Linhai
Xu, Qingbiao
author_sort Zong, Xiaomei
collection PubMed
description A well-known milk-derived bioactive tripeptide, VPP (Val-Pro-Pro) has good anti-inflammatory, anti-hypertension, and anti-hydrolysis properties. However, whether VPP can alleviate calf intestinal inflammation is unclear. In this experiment, the effects of VPP on growth, diarrhea incidence, serum biochemical indices, short-chain fatty acids, and fecal microorganisms were examined in pre-weaning Holstein calves. Eighteen calves with similar birth date, body weight, and genetic background were randomly assigned equally to two groups (n = 9). The control group was given 50 mL of phosphate buffer saline before morning feeding, whereas the VPP group received 50 mL of VPP solution (100 mg/kg body weight/d). The study lasted for 17 days, with the first 3 days used for adaptation. Initial and final body weights were determined, and daily dry matter intake and fecal score were recorded throughout the study. Serum hormone levels and antioxidant and immune indices were measured on day 14. Fecal microorganisms were collected on days 0, 7, and 14, and 16S rDNA sequencing was performed. Oral administration of VPP did not significantly affect calf average daily feed intake and body weight, but the growth rate in body weight was significantly higher in the VPP group than in the control group on day 7 (P < 0.05). Compared with the control, VPP significantly decreased serum TNF-α and IL-6 contents (P < 0.05), and concentrations of nitric oxide and IL-1β also decreased but not significantly (0.05 < P < 0.1). After seven days of VPP, relative abundances of g_Lachnoclostridium, uncultured_bacterium_, and g_Streptococcus in fecal samples increased significantly (P < 0.05). Compared with the control, VPP significantly increased concentrations of the fecal short-chain fatty acids n-butyric acid and isovaleric acid (P < 0.05). In conclusion, VPP can relieve intestinal inflammation and alleviate the degree of diarrhea in pre-weaning calves.
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spelling pubmed-100909562023-04-13 Effects of milk-derived bioactive peptide VPP on diarrhea of pre-weaning calves Zong, Xiaomei Gao, Ya Du, Yufeng Hou, Jinxiu Yang, Linhai Xu, Qingbiao Front Vet Sci Veterinary Science A well-known milk-derived bioactive tripeptide, VPP (Val-Pro-Pro) has good anti-inflammatory, anti-hypertension, and anti-hydrolysis properties. However, whether VPP can alleviate calf intestinal inflammation is unclear. In this experiment, the effects of VPP on growth, diarrhea incidence, serum biochemical indices, short-chain fatty acids, and fecal microorganisms were examined in pre-weaning Holstein calves. Eighteen calves with similar birth date, body weight, and genetic background were randomly assigned equally to two groups (n = 9). The control group was given 50 mL of phosphate buffer saline before morning feeding, whereas the VPP group received 50 mL of VPP solution (100 mg/kg body weight/d). The study lasted for 17 days, with the first 3 days used for adaptation. Initial and final body weights were determined, and daily dry matter intake and fecal score were recorded throughout the study. Serum hormone levels and antioxidant and immune indices were measured on day 14. Fecal microorganisms were collected on days 0, 7, and 14, and 16S rDNA sequencing was performed. Oral administration of VPP did not significantly affect calf average daily feed intake and body weight, but the growth rate in body weight was significantly higher in the VPP group than in the control group on day 7 (P < 0.05). Compared with the control, VPP significantly decreased serum TNF-α and IL-6 contents (P < 0.05), and concentrations of nitric oxide and IL-1β also decreased but not significantly (0.05 < P < 0.1). After seven days of VPP, relative abundances of g_Lachnoclostridium, uncultured_bacterium_, and g_Streptococcus in fecal samples increased significantly (P < 0.05). Compared with the control, VPP significantly increased concentrations of the fecal short-chain fatty acids n-butyric acid and isovaleric acid (P < 0.05). In conclusion, VPP can relieve intestinal inflammation and alleviate the degree of diarrhea in pre-weaning calves. Frontiers Media S.A. 2023-03-29 /pmc/articles/PMC10090956/ /pubmed/37065247 http://dx.doi.org/10.3389/fvets.2023.1154197 Text en Copyright © 2023 Zong, Gao, Du, Hou, Yang and Xu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Zong, Xiaomei
Gao, Ya
Du, Yufeng
Hou, Jinxiu
Yang, Linhai
Xu, Qingbiao
Effects of milk-derived bioactive peptide VPP on diarrhea of pre-weaning calves
title Effects of milk-derived bioactive peptide VPP on diarrhea of pre-weaning calves
title_full Effects of milk-derived bioactive peptide VPP on diarrhea of pre-weaning calves
title_fullStr Effects of milk-derived bioactive peptide VPP on diarrhea of pre-weaning calves
title_full_unstemmed Effects of milk-derived bioactive peptide VPP on diarrhea of pre-weaning calves
title_short Effects of milk-derived bioactive peptide VPP on diarrhea of pre-weaning calves
title_sort effects of milk-derived bioactive peptide vpp on diarrhea of pre-weaning calves
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10090956/
https://www.ncbi.nlm.nih.gov/pubmed/37065247
http://dx.doi.org/10.3389/fvets.2023.1154197
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