Cargando…

Whole genome analysis of a novel Spodoptera exigua nucleopolyhedrovirus isolate (SeMNPV-IR) to Iran

Baculoviruses are successful microbial control agents used in the biological control of agricultural pest species, especially in the order Lepidoptera. The beet armyworm, Spodoptera exigua is a popular agricultural pest in the world. S exigua larvae, which are active in the all-summer period, cause...

Descripción completa

Detalles Bibliográficos
Autores principales: Eroglu, Gozde Busra, Karimi, Javad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10091331/
https://www.ncbi.nlm.nih.gov/pubmed/37363644
http://dx.doi.org/10.1007/s11756-023-01399-2
_version_ 1785023113094758400
author Eroglu, Gozde Busra
Karimi, Javad
author_facet Eroglu, Gozde Busra
Karimi, Javad
author_sort Eroglu, Gozde Busra
collection PubMed
description Baculoviruses are successful microbial control agents used in the biological control of agricultural pest species, especially in the order Lepidoptera. The beet armyworm, Spodoptera exigua is a popular agricultural pest in the world. S exigua larvae, which are active in the all-summer period, cause economic losses by damaging many crops in agricultural production areas. This article aims to analyze the full genome of Spodoptera exigua multiple nucleopolyhedroviruses from Iran (SeMNPV-IR) and to determine the geographical difference between the strains at the genomic level. The full genome of SeMNPV-IR is 135.764 base pairs in length that contained 136 open reading frames (ORFs), and 43.92% G + C content. The seven homologous repeated (hr) regions were identified. In the results of genome-wide phylogenetic analysis, it was determined that the SeMNPV-IR genome isolated from Iran was interestingly close to the genome of the US and Korea isolates. However, there are significant differences in the two hypothetical (Orf 83 and Orf 104) genes. The SeMNPV-IR has a unique homolog repeat region (hr1, 96 bp) that is not found in other SeMNPV genomes, and it also differs in terms of the hr2 region. In silico restriction endonuclease analysis by StuI and SacII enzymes show that there were significant differences between all geographic isolates of SeMNPV. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11756-023-01399-2.
format Online
Article
Text
id pubmed-10091331
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Springer International Publishing
record_format MEDLINE/PubMed
spelling pubmed-100913312023-04-14 Whole genome analysis of a novel Spodoptera exigua nucleopolyhedrovirus isolate (SeMNPV-IR) to Iran Eroglu, Gozde Busra Karimi, Javad Biologia (Bratisl) Original Article Baculoviruses are successful microbial control agents used in the biological control of agricultural pest species, especially in the order Lepidoptera. The beet armyworm, Spodoptera exigua is a popular agricultural pest in the world. S exigua larvae, which are active in the all-summer period, cause economic losses by damaging many crops in agricultural production areas. This article aims to analyze the full genome of Spodoptera exigua multiple nucleopolyhedroviruses from Iran (SeMNPV-IR) and to determine the geographical difference between the strains at the genomic level. The full genome of SeMNPV-IR is 135.764 base pairs in length that contained 136 open reading frames (ORFs), and 43.92% G + C content. The seven homologous repeated (hr) regions were identified. In the results of genome-wide phylogenetic analysis, it was determined that the SeMNPV-IR genome isolated from Iran was interestingly close to the genome of the US and Korea isolates. However, there are significant differences in the two hypothetical (Orf 83 and Orf 104) genes. The SeMNPV-IR has a unique homolog repeat region (hr1, 96 bp) that is not found in other SeMNPV genomes, and it also differs in terms of the hr2 region. In silico restriction endonuclease analysis by StuI and SacII enzymes show that there were significant differences between all geographic isolates of SeMNPV. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11756-023-01399-2. Springer International Publishing 2023-04-12 /pmc/articles/PMC10091331/ /pubmed/37363644 http://dx.doi.org/10.1007/s11756-023-01399-2 Text en © The Author(s), under exclusive licence to Plant Science and Biodiversity Centre, Slovak Academy of Sciences (SAS), Institute of Zoology, Slovak Academy of Sciences (SAS), Institute of Molecular Biology, Slovak Academy of Sciences (SAS) 2023, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Article
Eroglu, Gozde Busra
Karimi, Javad
Whole genome analysis of a novel Spodoptera exigua nucleopolyhedrovirus isolate (SeMNPV-IR) to Iran
title Whole genome analysis of a novel Spodoptera exigua nucleopolyhedrovirus isolate (SeMNPV-IR) to Iran
title_full Whole genome analysis of a novel Spodoptera exigua nucleopolyhedrovirus isolate (SeMNPV-IR) to Iran
title_fullStr Whole genome analysis of a novel Spodoptera exigua nucleopolyhedrovirus isolate (SeMNPV-IR) to Iran
title_full_unstemmed Whole genome analysis of a novel Spodoptera exigua nucleopolyhedrovirus isolate (SeMNPV-IR) to Iran
title_short Whole genome analysis of a novel Spodoptera exigua nucleopolyhedrovirus isolate (SeMNPV-IR) to Iran
title_sort whole genome analysis of a novel spodoptera exigua nucleopolyhedrovirus isolate (semnpv-ir) to iran
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10091331/
https://www.ncbi.nlm.nih.gov/pubmed/37363644
http://dx.doi.org/10.1007/s11756-023-01399-2
work_keys_str_mv AT eroglugozdebusra wholegenomeanalysisofanovelspodopteraexiguanucleopolyhedrovirusisolatesemnpvirtoiran
AT karimijavad wholegenomeanalysisofanovelspodopteraexiguanucleopolyhedrovirusisolatesemnpvirtoiran