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NbThermo: a new thermostability database for nanobodies
We present NbThermo—a first-in-class database that collects melting temperatures (T(m)), amino acid sequences and several other categories of useful data for hundreds of nanobodies (Nbs), compiled from an extensive literature search. This so-far unique database currently contains up-to-date, manuall...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10091358/ https://www.ncbi.nlm.nih.gov/pubmed/37042467 http://dx.doi.org/10.1093/database/baad021 |
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author | Valdés-Tresanco, Mario S Valdés-Tresanco, Mario E Molina-Abad, Esteban Moreno, Ernesto |
author_facet | Valdés-Tresanco, Mario S Valdés-Tresanco, Mario E Molina-Abad, Esteban Moreno, Ernesto |
author_sort | Valdés-Tresanco, Mario S |
collection | PubMed |
description | We present NbThermo—a first-in-class database that collects melting temperatures (T(m)), amino acid sequences and several other categories of useful data for hundreds of nanobodies (Nbs), compiled from an extensive literature search. This so-far unique database currently contains up-to-date, manually curated data for 564 Nbs. It represents a contribution to efforts aimed at developing new algorithms for reliable T(m) prediction to assist Nb engineering for a wide range of applications of these unique biomolecules. Nbs from the two most common source organisms—llama and camel—show similar distributions of melting temperatures. A first exploratory research that takes advantage of this large data collection evidences that understanding the structural bases of Nb thermostability is a complex task, since there are no apparent differences in sequence patterns between the frameworks of Nbs with lower and higher melting temperatures, indicating that the highly variable loops play a relevant role in defining Nb thermostability. Database URL https://valdes-tresanco-ms.github.io/NbThermo |
format | Online Article Text |
id | pubmed-10091358 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-100913582023-04-13 NbThermo: a new thermostability database for nanobodies Valdés-Tresanco, Mario S Valdés-Tresanco, Mario E Molina-Abad, Esteban Moreno, Ernesto Database (Oxford) Original Article We present NbThermo—a first-in-class database that collects melting temperatures (T(m)), amino acid sequences and several other categories of useful data for hundreds of nanobodies (Nbs), compiled from an extensive literature search. This so-far unique database currently contains up-to-date, manually curated data for 564 Nbs. It represents a contribution to efforts aimed at developing new algorithms for reliable T(m) prediction to assist Nb engineering for a wide range of applications of these unique biomolecules. Nbs from the two most common source organisms—llama and camel—show similar distributions of melting temperatures. A first exploratory research that takes advantage of this large data collection evidences that understanding the structural bases of Nb thermostability is a complex task, since there are no apparent differences in sequence patterns between the frameworks of Nbs with lower and higher melting temperatures, indicating that the highly variable loops play a relevant role in defining Nb thermostability. Database URL https://valdes-tresanco-ms.github.io/NbThermo Oxford University Press 2023-04-12 /pmc/articles/PMC10091358/ /pubmed/37042467 http://dx.doi.org/10.1093/database/baad021 Text en © The Author(s) 2023. Published by Oxford University Press. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Valdés-Tresanco, Mario S Valdés-Tresanco, Mario E Molina-Abad, Esteban Moreno, Ernesto NbThermo: a new thermostability database for nanobodies |
title | NbThermo: a new thermostability database for nanobodies |
title_full | NbThermo: a new thermostability database for nanobodies |
title_fullStr | NbThermo: a new thermostability database for nanobodies |
title_full_unstemmed | NbThermo: a new thermostability database for nanobodies |
title_short | NbThermo: a new thermostability database for nanobodies |
title_sort | nbthermo: a new thermostability database for nanobodies |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10091358/ https://www.ncbi.nlm.nih.gov/pubmed/37042467 http://dx.doi.org/10.1093/database/baad021 |
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