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NbThermo: a new thermostability database for nanobodies

We present NbThermo—a first-in-class database that collects melting temperatures (T(m)), amino acid sequences and several other categories of useful data for hundreds of nanobodies (Nbs), compiled from an extensive literature search. This so-far unique database currently contains up-to-date, manuall...

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Detalles Bibliográficos
Autores principales: Valdés-Tresanco, Mario S, Valdés-Tresanco, Mario E, Molina-Abad, Esteban, Moreno, Ernesto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10091358/
https://www.ncbi.nlm.nih.gov/pubmed/37042467
http://dx.doi.org/10.1093/database/baad021
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author Valdés-Tresanco, Mario S
Valdés-Tresanco, Mario E
Molina-Abad, Esteban
Moreno, Ernesto
author_facet Valdés-Tresanco, Mario S
Valdés-Tresanco, Mario E
Molina-Abad, Esteban
Moreno, Ernesto
author_sort Valdés-Tresanco, Mario S
collection PubMed
description We present NbThermo—a first-in-class database that collects melting temperatures (T(m)), amino acid sequences and several other categories of useful data for hundreds of nanobodies (Nbs), compiled from an extensive literature search. This so-far unique database currently contains up-to-date, manually curated data for 564 Nbs. It represents a contribution to efforts aimed at developing new algorithms for reliable T(m) prediction to assist Nb engineering for a wide range of applications of these unique biomolecules. Nbs from the two most common source organisms—llama and camel—show similar distributions of melting temperatures. A first exploratory research that takes advantage of this large data collection evidences that understanding the structural bases of Nb thermostability is a complex task, since there are no apparent differences in sequence patterns between the frameworks of Nbs with lower and higher melting temperatures, indicating that the highly variable loops play a relevant role in defining Nb thermostability. Database URL https://valdes-tresanco-ms.github.io/NbThermo
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spelling pubmed-100913582023-04-13 NbThermo: a new thermostability database for nanobodies Valdés-Tresanco, Mario S Valdés-Tresanco, Mario E Molina-Abad, Esteban Moreno, Ernesto Database (Oxford) Original Article We present NbThermo—a first-in-class database that collects melting temperatures (T(m)), amino acid sequences and several other categories of useful data for hundreds of nanobodies (Nbs), compiled from an extensive literature search. This so-far unique database currently contains up-to-date, manually curated data for 564 Nbs. It represents a contribution to efforts aimed at developing new algorithms for reliable T(m) prediction to assist Nb engineering for a wide range of applications of these unique biomolecules. Nbs from the two most common source organisms—llama and camel—show similar distributions of melting temperatures. A first exploratory research that takes advantage of this large data collection evidences that understanding the structural bases of Nb thermostability is a complex task, since there are no apparent differences in sequence patterns between the frameworks of Nbs with lower and higher melting temperatures, indicating that the highly variable loops play a relevant role in defining Nb thermostability. Database URL https://valdes-tresanco-ms.github.io/NbThermo Oxford University Press 2023-04-12 /pmc/articles/PMC10091358/ /pubmed/37042467 http://dx.doi.org/10.1093/database/baad021 Text en © The Author(s) 2023. Published by Oxford University Press. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Valdés-Tresanco, Mario S
Valdés-Tresanco, Mario E
Molina-Abad, Esteban
Moreno, Ernesto
NbThermo: a new thermostability database for nanobodies
title NbThermo: a new thermostability database for nanobodies
title_full NbThermo: a new thermostability database for nanobodies
title_fullStr NbThermo: a new thermostability database for nanobodies
title_full_unstemmed NbThermo: a new thermostability database for nanobodies
title_short NbThermo: a new thermostability database for nanobodies
title_sort nbthermo: a new thermostability database for nanobodies
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10091358/
https://www.ncbi.nlm.nih.gov/pubmed/37042467
http://dx.doi.org/10.1093/database/baad021
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