Cargando…
Virally-induced expression of GABA(A) receptor δ subunits following their pathological loss reveals their role in regulating GABA(A) receptor assembly
Decreased expression of the δ subunit of the GABA(A) receptor (GABA(A)R) has been found in the dentate gyrus in several animal models of epilepsy and other disorders with increased excitability and is associated with altered modulation of tonic inhibition in dentate granule cells (GCs). In contrast,...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10091858/ https://www.ncbi.nlm.nih.gov/pubmed/35934131 http://dx.doi.org/10.1016/j.pneurobio.2022.102337 |
_version_ | 1785023215369715712 |
---|---|
author | Sun, Yu Peng, Zechun Wei, Xiaofei Zhang, Nianhui Huang, Christine S. Wallner, Martin Mody, Istvan Houser, Carolyn R. |
author_facet | Sun, Yu Peng, Zechun Wei, Xiaofei Zhang, Nianhui Huang, Christine S. Wallner, Martin Mody, Istvan Houser, Carolyn R. |
author_sort | Sun, Yu |
collection | PubMed |
description | Decreased expression of the δ subunit of the GABA(A) receptor (GABA(A)R) has been found in the dentate gyrus in several animal models of epilepsy and other disorders with increased excitability and is associated with altered modulation of tonic inhibition in dentate granule cells (GCs). In contrast, other GABA(A)R subunits, including α4 and γ2 subunits, are increased, but the relationship between these changes is unclear. The goals of this study were to determine if viral transfection of δ subunits in dentate GCs could increase δ subunit expression, alter expression of potentially-related GABA(A)R subunits, and restore more normal network excitability in the dentate gyrus in a mouse model of epilepsy. Pilocarpine-induced seizures were elicited in DOCK10-Cre mice that express Cre selectively in dentate GCs, and two weeks later the mice were injected unilaterally with a Cre-dependent δ-GABA(A)R viral vector. At 4–6 weeks following transfection, δ subunit immunolabeling was substantially increased in dentate GCs on the transfected side compared to the nontransfected side. Importantly, α4 and γ2 subunit labeling was downregulated on the transfected side. Electrophysiological studies revealed enhanced tonic inhibition, decreased network excitability, and increased neurosteroid sensitivity in slices from the δ subunit-transfected side compared to those from the nontransfected side of the same pilocarpine-treated animal, consistent with the formation of δ subunit-containing GABA(A)Rs. No differences were observed between sides of eYFP-transfected animals. These findings are consistent with the idea that altering expression of key subunits, such as the δ subunit, regulates GABA(A)R subunit assemblies, resulting in substantial effects on network excitability. |
format | Online Article Text |
id | pubmed-10091858 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
record_format | MEDLINE/PubMed |
spelling | pubmed-100918582023-04-12 Virally-induced expression of GABA(A) receptor δ subunits following their pathological loss reveals their role in regulating GABA(A) receptor assembly Sun, Yu Peng, Zechun Wei, Xiaofei Zhang, Nianhui Huang, Christine S. Wallner, Martin Mody, Istvan Houser, Carolyn R. Prog Neurobiol Article Decreased expression of the δ subunit of the GABA(A) receptor (GABA(A)R) has been found in the dentate gyrus in several animal models of epilepsy and other disorders with increased excitability and is associated with altered modulation of tonic inhibition in dentate granule cells (GCs). In contrast, other GABA(A)R subunits, including α4 and γ2 subunits, are increased, but the relationship between these changes is unclear. The goals of this study were to determine if viral transfection of δ subunits in dentate GCs could increase δ subunit expression, alter expression of potentially-related GABA(A)R subunits, and restore more normal network excitability in the dentate gyrus in a mouse model of epilepsy. Pilocarpine-induced seizures were elicited in DOCK10-Cre mice that express Cre selectively in dentate GCs, and two weeks later the mice were injected unilaterally with a Cre-dependent δ-GABA(A)R viral vector. At 4–6 weeks following transfection, δ subunit immunolabeling was substantially increased in dentate GCs on the transfected side compared to the nontransfected side. Importantly, α4 and γ2 subunit labeling was downregulated on the transfected side. Electrophysiological studies revealed enhanced tonic inhibition, decreased network excitability, and increased neurosteroid sensitivity in slices from the δ subunit-transfected side compared to those from the nontransfected side of the same pilocarpine-treated animal, consistent with the formation of δ subunit-containing GABA(A)Rs. No differences were observed between sides of eYFP-transfected animals. These findings are consistent with the idea that altering expression of key subunits, such as the δ subunit, regulates GABA(A)R subunit assemblies, resulting in substantial effects on network excitability. 2022-11 2022-08-05 /pmc/articles/PMC10091858/ /pubmed/35934131 http://dx.doi.org/10.1016/j.pneurobio.2022.102337 Text en https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Sun, Yu Peng, Zechun Wei, Xiaofei Zhang, Nianhui Huang, Christine S. Wallner, Martin Mody, Istvan Houser, Carolyn R. Virally-induced expression of GABA(A) receptor δ subunits following their pathological loss reveals their role in regulating GABA(A) receptor assembly |
title | Virally-induced expression of GABA(A) receptor δ subunits following their pathological loss reveals their role in regulating GABA(A) receptor assembly |
title_full | Virally-induced expression of GABA(A) receptor δ subunits following their pathological loss reveals their role in regulating GABA(A) receptor assembly |
title_fullStr | Virally-induced expression of GABA(A) receptor δ subunits following their pathological loss reveals their role in regulating GABA(A) receptor assembly |
title_full_unstemmed | Virally-induced expression of GABA(A) receptor δ subunits following their pathological loss reveals their role in regulating GABA(A) receptor assembly |
title_short | Virally-induced expression of GABA(A) receptor δ subunits following their pathological loss reveals their role in regulating GABA(A) receptor assembly |
title_sort | virally-induced expression of gaba(a) receptor δ subunits following their pathological loss reveals their role in regulating gaba(a) receptor assembly |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10091858/ https://www.ncbi.nlm.nih.gov/pubmed/35934131 http://dx.doi.org/10.1016/j.pneurobio.2022.102337 |
work_keys_str_mv | AT sunyu virallyinducedexpressionofgabaareceptordsubunitsfollowingtheirpathologicallossrevealstheirroleinregulatinggabaareceptorassembly AT pengzechun virallyinducedexpressionofgabaareceptordsubunitsfollowingtheirpathologicallossrevealstheirroleinregulatinggabaareceptorassembly AT weixiaofei virallyinducedexpressionofgabaareceptordsubunitsfollowingtheirpathologicallossrevealstheirroleinregulatinggabaareceptorassembly AT zhangnianhui virallyinducedexpressionofgabaareceptordsubunitsfollowingtheirpathologicallossrevealstheirroleinregulatinggabaareceptorassembly AT huangchristines virallyinducedexpressionofgabaareceptordsubunitsfollowingtheirpathologicallossrevealstheirroleinregulatinggabaareceptorassembly AT wallnermartin virallyinducedexpressionofgabaareceptordsubunitsfollowingtheirpathologicallossrevealstheirroleinregulatinggabaareceptorassembly AT modyistvan virallyinducedexpressionofgabaareceptordsubunitsfollowingtheirpathologicallossrevealstheirroleinregulatinggabaareceptorassembly AT housercarolynr virallyinducedexpressionofgabaareceptordsubunitsfollowingtheirpathologicallossrevealstheirroleinregulatinggabaareceptorassembly |