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Comparing invasive and noninvasive faecal sampling in wildlife microbiome studies: A case study on wild common cranes
In ecological and conservation studies, responsible researchers strive to obtain rich data while minimizing disturbance to wildlife and ecosystems. We assessed if samples collected noninvasively can be used for faecal microbiome research, comparing microbiota of noninvasively collected faecal sample...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10091961/ https://www.ncbi.nlm.nih.gov/pubmed/36039836 http://dx.doi.org/10.1111/1755-0998.13708 |
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author | Turjeman, Sondra Pekarsky, Sasha Corl, Ammon Kamath, Pauline L. Getz, Wayne M. Bowie, Rauri C. K. Markin, Yuri Nathan, Ran |
author_facet | Turjeman, Sondra Pekarsky, Sasha Corl, Ammon Kamath, Pauline L. Getz, Wayne M. Bowie, Rauri C. K. Markin, Yuri Nathan, Ran |
author_sort | Turjeman, Sondra |
collection | PubMed |
description | In ecological and conservation studies, responsible researchers strive to obtain rich data while minimizing disturbance to wildlife and ecosystems. We assessed if samples collected noninvasively can be used for faecal microbiome research, comparing microbiota of noninvasively collected faecal samples to those collected from trapped common cranes at the same sites over the same periods. We found significant differences in faecal microbial composition (alpha and beta diversity), which likely did not result from noninvasive sample exposure to soil contaminants, as assessed by comparing bacterial oxygen use profiles. Differences might result from trapped birds' exposure to sedatives or stress. We conclude that if all samples are collected in the same manner, comparative analyses are valid, and noninvasive sampling may better represent host faecal microbiota because there are no trapping effects. Experiments with fresh and delayed sample collection can elucidate effects of environmental exposures on microbiota. Further, controlled tests of stressing or sedation may unravel how trapping affects wildlife microbiota. |
format | Online Article Text |
id | pubmed-10091961 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100919612023-04-13 Comparing invasive and noninvasive faecal sampling in wildlife microbiome studies: A case study on wild common cranes Turjeman, Sondra Pekarsky, Sasha Corl, Ammon Kamath, Pauline L. Getz, Wayne M. Bowie, Rauri C. K. Markin, Yuri Nathan, Ran Mol Ecol Resour RESOURCE ARTICLES In ecological and conservation studies, responsible researchers strive to obtain rich data while minimizing disturbance to wildlife and ecosystems. We assessed if samples collected noninvasively can be used for faecal microbiome research, comparing microbiota of noninvasively collected faecal samples to those collected from trapped common cranes at the same sites over the same periods. We found significant differences in faecal microbial composition (alpha and beta diversity), which likely did not result from noninvasive sample exposure to soil contaminants, as assessed by comparing bacterial oxygen use profiles. Differences might result from trapped birds' exposure to sedatives or stress. We conclude that if all samples are collected in the same manner, comparative analyses are valid, and noninvasive sampling may better represent host faecal microbiota because there are no trapping effects. Experiments with fresh and delayed sample collection can elucidate effects of environmental exposures on microbiota. Further, controlled tests of stressing or sedation may unravel how trapping affects wildlife microbiota. John Wiley and Sons Inc. 2022-10-03 2023-02 /pmc/articles/PMC10091961/ /pubmed/36039836 http://dx.doi.org/10.1111/1755-0998.13708 Text en © 2022 The Authors. Molecular Ecology Resources published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | RESOURCE ARTICLES Turjeman, Sondra Pekarsky, Sasha Corl, Ammon Kamath, Pauline L. Getz, Wayne M. Bowie, Rauri C. K. Markin, Yuri Nathan, Ran Comparing invasive and noninvasive faecal sampling in wildlife microbiome studies: A case study on wild common cranes |
title | Comparing invasive and noninvasive faecal sampling in wildlife microbiome studies: A case study on wild common cranes |
title_full | Comparing invasive and noninvasive faecal sampling in wildlife microbiome studies: A case study on wild common cranes |
title_fullStr | Comparing invasive and noninvasive faecal sampling in wildlife microbiome studies: A case study on wild common cranes |
title_full_unstemmed | Comparing invasive and noninvasive faecal sampling in wildlife microbiome studies: A case study on wild common cranes |
title_short | Comparing invasive and noninvasive faecal sampling in wildlife microbiome studies: A case study on wild common cranes |
title_sort | comparing invasive and noninvasive faecal sampling in wildlife microbiome studies: a case study on wild common cranes |
topic | RESOURCE ARTICLES |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10091961/ https://www.ncbi.nlm.nih.gov/pubmed/36039836 http://dx.doi.org/10.1111/1755-0998.13708 |
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