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Adaptations to high pressure of Nautilia sp. strain PV‐1, a piezophilic Campylobacterium (aka Epsilonproteobacterium) isolated from a deep‐sea hydrothermal vent

Physiological and gene expression studies of deep‐sea bacteria under pressure conditions similar to those experienced in their natural habitat are critical for understanding growth kinetics and metabolic adaptations to in situ conditions. The Campylobacterium (aka Epsilonproteobacterium) Nautilia sp...

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Autores principales: Smedile, Francesco, Foustoukos, Dionysis I., Patwardhan, Sushmita, Mullane, Kelli, Schlegel, Ian, Adams, Michael W., Schut, Gerrit J., Giovannelli, Donato, Vetriani, Costantino
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10092268/
https://www.ncbi.nlm.nih.gov/pubmed/36271901
http://dx.doi.org/10.1111/1462-2920.16256
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author Smedile, Francesco
Foustoukos, Dionysis I.
Patwardhan, Sushmita
Mullane, Kelli
Schlegel, Ian
Adams, Michael W.
Schut, Gerrit J.
Giovannelli, Donato
Vetriani, Costantino
author_facet Smedile, Francesco
Foustoukos, Dionysis I.
Patwardhan, Sushmita
Mullane, Kelli
Schlegel, Ian
Adams, Michael W.
Schut, Gerrit J.
Giovannelli, Donato
Vetriani, Costantino
author_sort Smedile, Francesco
collection PubMed
description Physiological and gene expression studies of deep‐sea bacteria under pressure conditions similar to those experienced in their natural habitat are critical for understanding growth kinetics and metabolic adaptations to in situ conditions. The Campylobacterium (aka Epsilonproteobacterium) Nautilia sp. strain PV‐1 was isolated from hydrothermal fluids released from an active deep‐sea hydrothermal vent at 9° N on the East Pacific Rise. Strain PV‐1 is a piezophilic, moderately thermophilic, chemolithoautotrophic anaerobe that conserves energy by coupling the oxidation of hydrogen to the reduction of nitrate or elemental sulfur. Using a high‐pressure–high temperature continuous culture system, we established that strain PV‐1 has the shortest generation time of all known piezophilic bacteria and we investigated its protein expression pattern in response to different hydrostatic pressure regimes. Proteogenomic analyses of strain PV‐1 grown at 20 and 5 MPa showed that pressure adaptation is not restricted to stress response or homeoviscous adaptation but extends to enzymes involved in central metabolic pathways. Protein synthesis, motility, transport, and energy metabolism are all affected by pressure, although to different extents. In strain PV‐1, low‐pressure conditions induce the synthesis of phage‐related proteins and an overexpression of enzymes involved in carbon fixation.
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spelling pubmed-100922682023-04-13 Adaptations to high pressure of Nautilia sp. strain PV‐1, a piezophilic Campylobacterium (aka Epsilonproteobacterium) isolated from a deep‐sea hydrothermal vent Smedile, Francesco Foustoukos, Dionysis I. Patwardhan, Sushmita Mullane, Kelli Schlegel, Ian Adams, Michael W. Schut, Gerrit J. Giovannelli, Donato Vetriani, Costantino Environ Microbiol Research Articles Physiological and gene expression studies of deep‐sea bacteria under pressure conditions similar to those experienced in their natural habitat are critical for understanding growth kinetics and metabolic adaptations to in situ conditions. The Campylobacterium (aka Epsilonproteobacterium) Nautilia sp. strain PV‐1 was isolated from hydrothermal fluids released from an active deep‐sea hydrothermal vent at 9° N on the East Pacific Rise. Strain PV‐1 is a piezophilic, moderately thermophilic, chemolithoautotrophic anaerobe that conserves energy by coupling the oxidation of hydrogen to the reduction of nitrate or elemental sulfur. Using a high‐pressure–high temperature continuous culture system, we established that strain PV‐1 has the shortest generation time of all known piezophilic bacteria and we investigated its protein expression pattern in response to different hydrostatic pressure regimes. Proteogenomic analyses of strain PV‐1 grown at 20 and 5 MPa showed that pressure adaptation is not restricted to stress response or homeoviscous adaptation but extends to enzymes involved in central metabolic pathways. Protein synthesis, motility, transport, and energy metabolism are all affected by pressure, although to different extents. In strain PV‐1, low‐pressure conditions induce the synthesis of phage‐related proteins and an overexpression of enzymes involved in carbon fixation. John Wiley & Sons, Inc. 2022-10-31 2022-12 /pmc/articles/PMC10092268/ /pubmed/36271901 http://dx.doi.org/10.1111/1462-2920.16256 Text en © 2022 The Authors. Environmental Microbiology published by Applied Microbiology International and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Smedile, Francesco
Foustoukos, Dionysis I.
Patwardhan, Sushmita
Mullane, Kelli
Schlegel, Ian
Adams, Michael W.
Schut, Gerrit J.
Giovannelli, Donato
Vetriani, Costantino
Adaptations to high pressure of Nautilia sp. strain PV‐1, a piezophilic Campylobacterium (aka Epsilonproteobacterium) isolated from a deep‐sea hydrothermal vent
title Adaptations to high pressure of Nautilia sp. strain PV‐1, a piezophilic Campylobacterium (aka Epsilonproteobacterium) isolated from a deep‐sea hydrothermal vent
title_full Adaptations to high pressure of Nautilia sp. strain PV‐1, a piezophilic Campylobacterium (aka Epsilonproteobacterium) isolated from a deep‐sea hydrothermal vent
title_fullStr Adaptations to high pressure of Nautilia sp. strain PV‐1, a piezophilic Campylobacterium (aka Epsilonproteobacterium) isolated from a deep‐sea hydrothermal vent
title_full_unstemmed Adaptations to high pressure of Nautilia sp. strain PV‐1, a piezophilic Campylobacterium (aka Epsilonproteobacterium) isolated from a deep‐sea hydrothermal vent
title_short Adaptations to high pressure of Nautilia sp. strain PV‐1, a piezophilic Campylobacterium (aka Epsilonproteobacterium) isolated from a deep‐sea hydrothermal vent
title_sort adaptations to high pressure of nautilia sp. strain pv‐1, a piezophilic campylobacterium (aka epsilonproteobacterium) isolated from a deep‐sea hydrothermal vent
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10092268/
https://www.ncbi.nlm.nih.gov/pubmed/36271901
http://dx.doi.org/10.1111/1462-2920.16256
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