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Alterations in microglial morphology concentrate in the habitual sleeping period of the mouse

In nocturnal animals, waking appears during the dark period while maximal non‐rapid‐eye‐movement sleep (NREMS) with electroencephalographic slow‐wave‐activity (SWA) takes place at the beginning of the light period. Vigilance states associate with variable levels of neuronal activity: waking with hig...

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Autores principales: Steffens, Sarah Katharina, Stenberg, Tarja Helena, Wigren, Henna‐Kaisa Margareta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10092278/
https://www.ncbi.nlm.nih.gov/pubmed/36196985
http://dx.doi.org/10.1002/glia.24279
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author Steffens, Sarah Katharina
Stenberg, Tarja Helena
Wigren, Henna‐Kaisa Margareta
author_facet Steffens, Sarah Katharina
Stenberg, Tarja Helena
Wigren, Henna‐Kaisa Margareta
author_sort Steffens, Sarah Katharina
collection PubMed
description In nocturnal animals, waking appears during the dark period while maximal non‐rapid‐eye‐movement sleep (NREMS) with electroencephalographic slow‐wave‐activity (SWA) takes place at the beginning of the light period. Vigilance states associate with variable levels of neuronal activity: waking with high‐frequency activity patterns while during NREMS, SWA influences neuronal activity in many brain areas. On a glial level, sleep deprivation modifies microglial morphology, but only few studies have investigated microglia through the physiological sleep–wake cycle. To quantify microglial morphology (territory, volume, ramification) throughout the 24 h light–dark cycle, we collected brain samples from inbred C57BL male mice (n = 51) every 3 h and applied a 3D‐reconstruction method for microglial cells on the acquired confocal microscopy images. As microglia express regional heterogeneity and are influenced by local neuronal activity, we chose to investigate three interconnected and functionally well‐characterized brain areas: the somatosensory cortex (SC), the dorsal hippocampus (HC), and the basal forebrain (BF). To temporally associate microglial morphology with vigilance stages, we performed a 24 h polysomnography in a separate group of animals (n = 6). In line with previous findings, microglia displayed de‐ramification in the 12 h light‐ and hyper‐ramification in the 12 h dark period. Notably, we found that the decrease in microglial features was most prominent within the early hours of the light period, co‐occurring with maximal sleep SWA. By the end of the light period, all features reached maximum levels and remained steadily elevated throughout the dark period with minor regional differences. We propose that vigilance‐stage specific neuronal activity, and SWA, could modify microglial morphology.
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spelling pubmed-100922782023-04-13 Alterations in microglial morphology concentrate in the habitual sleeping period of the mouse Steffens, Sarah Katharina Stenberg, Tarja Helena Wigren, Henna‐Kaisa Margareta Glia Research Articles In nocturnal animals, waking appears during the dark period while maximal non‐rapid‐eye‐movement sleep (NREMS) with electroencephalographic slow‐wave‐activity (SWA) takes place at the beginning of the light period. Vigilance states associate with variable levels of neuronal activity: waking with high‐frequency activity patterns while during NREMS, SWA influences neuronal activity in many brain areas. On a glial level, sleep deprivation modifies microglial morphology, but only few studies have investigated microglia through the physiological sleep–wake cycle. To quantify microglial morphology (territory, volume, ramification) throughout the 24 h light–dark cycle, we collected brain samples from inbred C57BL male mice (n = 51) every 3 h and applied a 3D‐reconstruction method for microglial cells on the acquired confocal microscopy images. As microglia express regional heterogeneity and are influenced by local neuronal activity, we chose to investigate three interconnected and functionally well‐characterized brain areas: the somatosensory cortex (SC), the dorsal hippocampus (HC), and the basal forebrain (BF). To temporally associate microglial morphology with vigilance stages, we performed a 24 h polysomnography in a separate group of animals (n = 6). In line with previous findings, microglia displayed de‐ramification in the 12 h light‐ and hyper‐ramification in the 12 h dark period. Notably, we found that the decrease in microglial features was most prominent within the early hours of the light period, co‐occurring with maximal sleep SWA. By the end of the light period, all features reached maximum levels and remained steadily elevated throughout the dark period with minor regional differences. We propose that vigilance‐stage specific neuronal activity, and SWA, could modify microglial morphology. John Wiley & Sons, Inc. 2022-10-05 2023-02 /pmc/articles/PMC10092278/ /pubmed/36196985 http://dx.doi.org/10.1002/glia.24279 Text en © 2022 The Authors. GLIA published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Steffens, Sarah Katharina
Stenberg, Tarja Helena
Wigren, Henna‐Kaisa Margareta
Alterations in microglial morphology concentrate in the habitual sleeping period of the mouse
title Alterations in microglial morphology concentrate in the habitual sleeping period of the mouse
title_full Alterations in microglial morphology concentrate in the habitual sleeping period of the mouse
title_fullStr Alterations in microglial morphology concentrate in the habitual sleeping period of the mouse
title_full_unstemmed Alterations in microglial morphology concentrate in the habitual sleeping period of the mouse
title_short Alterations in microglial morphology concentrate in the habitual sleeping period of the mouse
title_sort alterations in microglial morphology concentrate in the habitual sleeping period of the mouse
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10092278/
https://www.ncbi.nlm.nih.gov/pubmed/36196985
http://dx.doi.org/10.1002/glia.24279
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