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A Simple Colorimetric Assay of Bleomycin‐Mediated DNA Cleavage Utilizing Double‐Stranded DNA‐Modified Gold Nanoparticles

A colorimetric assay of DNA cleavage by bleomycin (BLM) derivatives was developed utilizing high colloidal stability on double‐stranded (ds) DNA‐modified gold nanoparticles (dsDNA‐AuNPs) possessing a cleavage site. The assay was performed using dsDNA‐AuNPs treated with inactive BLM or activated BLM...

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Autores principales: Akiyama, Yoshitsugu, Kimura, Kazunori, Komatsu, Syuuhei, Takarada, Tohru, Maeda, Mizuo, Kikuchi, Akihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10092608/
https://www.ncbi.nlm.nih.gov/pubmed/36156837
http://dx.doi.org/10.1002/cbic.202200451
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author Akiyama, Yoshitsugu
Kimura, Kazunori
Komatsu, Syuuhei
Takarada, Tohru
Maeda, Mizuo
Kikuchi, Akihiko
author_facet Akiyama, Yoshitsugu
Kimura, Kazunori
Komatsu, Syuuhei
Takarada, Tohru
Maeda, Mizuo
Kikuchi, Akihiko
author_sort Akiyama, Yoshitsugu
collection PubMed
description A colorimetric assay of DNA cleavage by bleomycin (BLM) derivatives was developed utilizing high colloidal stability on double‐stranded (ds) DNA‐modified gold nanoparticles (dsDNA‐AuNPs) possessing a cleavage site. The assay was performed using dsDNA‐AuNPs treated with inactive BLM or activated BLM (Fe(II)⋅BLM). A 10‐min exposure in dsDNA‐AuNPs with inactive BLM treatment resulted in a rapid color change from red to purple because of salt‐induced non‐crosslinking aggregation of dsDNA‐AuNPs. In contrast, the addition of active Fe(II)⋅BLM retained the red color, probably because of the formation of protruding structures at the outermost phase of dsDNA‐AuNPs caused by BLM‐mediated DNA cleavage. Furthermore, the results of our model experiments indicate that oxidative base release and DNA‐cleavage pathways could be visually distinguished with color change. The present methodology was also applicable to model screening assays using several drugs with different mechanisms related to antitumor activity. These results strongly suggest that this assay with a rapid color change could lead to simple and efficient screening of potent antitumor agents.
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spelling pubmed-100926082023-04-13 A Simple Colorimetric Assay of Bleomycin‐Mediated DNA Cleavage Utilizing Double‐Stranded DNA‐Modified Gold Nanoparticles Akiyama, Yoshitsugu Kimura, Kazunori Komatsu, Syuuhei Takarada, Tohru Maeda, Mizuo Kikuchi, Akihiko Chembiochem Research Articles A colorimetric assay of DNA cleavage by bleomycin (BLM) derivatives was developed utilizing high colloidal stability on double‐stranded (ds) DNA‐modified gold nanoparticles (dsDNA‐AuNPs) possessing a cleavage site. The assay was performed using dsDNA‐AuNPs treated with inactive BLM or activated BLM (Fe(II)⋅BLM). A 10‐min exposure in dsDNA‐AuNPs with inactive BLM treatment resulted in a rapid color change from red to purple because of salt‐induced non‐crosslinking aggregation of dsDNA‐AuNPs. In contrast, the addition of active Fe(II)⋅BLM retained the red color, probably because of the formation of protruding structures at the outermost phase of dsDNA‐AuNPs caused by BLM‐mediated DNA cleavage. Furthermore, the results of our model experiments indicate that oxidative base release and DNA‐cleavage pathways could be visually distinguished with color change. The present methodology was also applicable to model screening assays using several drugs with different mechanisms related to antitumor activity. These results strongly suggest that this assay with a rapid color change could lead to simple and efficient screening of potent antitumor agents. John Wiley and Sons Inc. 2022-10-25 2023-01-03 /pmc/articles/PMC10092608/ /pubmed/36156837 http://dx.doi.org/10.1002/cbic.202200451 Text en © 2022 The Authors. ChemBioChem published by Wiley-VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Akiyama, Yoshitsugu
Kimura, Kazunori
Komatsu, Syuuhei
Takarada, Tohru
Maeda, Mizuo
Kikuchi, Akihiko
A Simple Colorimetric Assay of Bleomycin‐Mediated DNA Cleavage Utilizing Double‐Stranded DNA‐Modified Gold Nanoparticles
title A Simple Colorimetric Assay of Bleomycin‐Mediated DNA Cleavage Utilizing Double‐Stranded DNA‐Modified Gold Nanoparticles
title_full A Simple Colorimetric Assay of Bleomycin‐Mediated DNA Cleavage Utilizing Double‐Stranded DNA‐Modified Gold Nanoparticles
title_fullStr A Simple Colorimetric Assay of Bleomycin‐Mediated DNA Cleavage Utilizing Double‐Stranded DNA‐Modified Gold Nanoparticles
title_full_unstemmed A Simple Colorimetric Assay of Bleomycin‐Mediated DNA Cleavage Utilizing Double‐Stranded DNA‐Modified Gold Nanoparticles
title_short A Simple Colorimetric Assay of Bleomycin‐Mediated DNA Cleavage Utilizing Double‐Stranded DNA‐Modified Gold Nanoparticles
title_sort simple colorimetric assay of bleomycin‐mediated dna cleavage utilizing double‐stranded dna‐modified gold nanoparticles
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10092608/
https://www.ncbi.nlm.nih.gov/pubmed/36156837
http://dx.doi.org/10.1002/cbic.202200451
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