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Demonstration of bacterium‐free very rapid reverse genetics system using mumps virus

The reverse genetics system is a very powerful tool for analyzing the molecular mechanisms of viral propagation and pathogenesis. However, full‐length genome plasmid construction is highly time‐consuming and laborious, and undesired mutations may be introduced by Escherichia coli. This study shows a...

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Detalles Bibliográficos
Autores principales: Bae, Chaewon, Katoh, Hiroshi, Wakata, Aika, Sakata, Masafumi, Kato, Fumihiro, Takeda, Makoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10092663/
https://www.ncbi.nlm.nih.gov/pubmed/36259144
http://dx.doi.org/10.1111/1348-0421.13032