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Immunohistochemical comparison of lateral bone augmentation using a synthetic TiO(2) block or a xenogeneic graft in chronic alveolar defects

OBJECTIVES: To evaluate osteogenic markers and alveolar ridge profile changes in guided bone regeneration (GBR) of chronic noncontained bone defects using a nonresorbable TiO(2) block. MATERIALS AND METHODS: Three buccal bone defects were created in each hemimandible of eight beagle dogs and allowed...

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Autores principales: Thieu, Minh Khai Le, Stoetzel, Sabine, Rahmati, Maryam, El Khassawna, Thaqif, Verket, Anders, Sanz‐Esporrin, Javier, Sanz, Mariano, Ellingsen, Jan Eirik, Haugen, Håvard Jostein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10092822/
https://www.ncbi.nlm.nih.gov/pubmed/36222116
http://dx.doi.org/10.1111/cid.13143
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author Thieu, Minh Khai Le
Stoetzel, Sabine
Rahmati, Maryam
El Khassawna, Thaqif
Verket, Anders
Sanz‐Esporrin, Javier
Sanz, Mariano
Ellingsen, Jan Eirik
Haugen, Håvard Jostein
author_facet Thieu, Minh Khai Le
Stoetzel, Sabine
Rahmati, Maryam
El Khassawna, Thaqif
Verket, Anders
Sanz‐Esporrin, Javier
Sanz, Mariano
Ellingsen, Jan Eirik
Haugen, Håvard Jostein
author_sort Thieu, Minh Khai Le
collection PubMed
description OBJECTIVES: To evaluate osteogenic markers and alveolar ridge profile changes in guided bone regeneration (GBR) of chronic noncontained bone defects using a nonresorbable TiO(2) block. MATERIALS AND METHODS: Three buccal bone defects were created in each hemimandible of eight beagle dogs and allowed to heal for 8 weeks before GBR. Treatment was assigned by block randomization: TiO(2) block: TiO(2)‐scaffold and a collagen membrane, DBBM particulates: Deproteinized bovine bone mineral (DBBM) and a collagen membrane, Empty control: Only collagen membrane. Bone regeneration was assessed on two different healing timepoints: early (4 weeks) and late healing (12 weeks) using several immunohistochemistry markers including alpha‐smooth muscle actin (α‐SMA), osteopontin, osteocalcin, tartrate‐resistant acid phosphatase, and collagen type I. Histomorphometry was performed on Movat Pentachrome‐stained and Von Kossa/Van Gieson‐stained sections. Stereolithographic (STL) models were used to compare alveolar profile changes. RESULTS: The percentage of α‐SMA and osteopontin increased in TiO(2) group after 12 weeks of healing at the bone‐scaffold interface, while collagen type I increased in the empty control group. In the defect area, α‐SMA decreased in the empty control group, while collagen type I increased in the DBBM group. All groups maintained alveolar profile from 4 to 12 weeks, but TiO(2) group demonstrated the widest soft tissue contour profile. CONCLUSIONS: The present findings suggested contact osteogenesis when GBR is performed with a TiO(2) block or DBBM particulates. The increase in osteopontin indicated a potential for bone formation beyond 12 weeks. The alveolar profile data indicated a sustained lateral increase in lateral bone augmentation using a TiO(2) block and a collagen membrane, as compared with DBBM and a collagen membrane or a collagen membrane alone.
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spelling pubmed-100928222023-04-13 Immunohistochemical comparison of lateral bone augmentation using a synthetic TiO(2) block or a xenogeneic graft in chronic alveolar defects Thieu, Minh Khai Le Stoetzel, Sabine Rahmati, Maryam El Khassawna, Thaqif Verket, Anders Sanz‐Esporrin, Javier Sanz, Mariano Ellingsen, Jan Eirik Haugen, Håvard Jostein Clin Implant Dent Relat Res Original Articles OBJECTIVES: To evaluate osteogenic markers and alveolar ridge profile changes in guided bone regeneration (GBR) of chronic noncontained bone defects using a nonresorbable TiO(2) block. MATERIALS AND METHODS: Three buccal bone defects were created in each hemimandible of eight beagle dogs and allowed to heal for 8 weeks before GBR. Treatment was assigned by block randomization: TiO(2) block: TiO(2)‐scaffold and a collagen membrane, DBBM particulates: Deproteinized bovine bone mineral (DBBM) and a collagen membrane, Empty control: Only collagen membrane. Bone regeneration was assessed on two different healing timepoints: early (4 weeks) and late healing (12 weeks) using several immunohistochemistry markers including alpha‐smooth muscle actin (α‐SMA), osteopontin, osteocalcin, tartrate‐resistant acid phosphatase, and collagen type I. Histomorphometry was performed on Movat Pentachrome‐stained and Von Kossa/Van Gieson‐stained sections. Stereolithographic (STL) models were used to compare alveolar profile changes. RESULTS: The percentage of α‐SMA and osteopontin increased in TiO(2) group after 12 weeks of healing at the bone‐scaffold interface, while collagen type I increased in the empty control group. In the defect area, α‐SMA decreased in the empty control group, while collagen type I increased in the DBBM group. All groups maintained alveolar profile from 4 to 12 weeks, but TiO(2) group demonstrated the widest soft tissue contour profile. CONCLUSIONS: The present findings suggested contact osteogenesis when GBR is performed with a TiO(2) block or DBBM particulates. The increase in osteopontin indicated a potential for bone formation beyond 12 weeks. The alveolar profile data indicated a sustained lateral increase in lateral bone augmentation using a TiO(2) block and a collagen membrane, as compared with DBBM and a collagen membrane or a collagen membrane alone. John Wiley & Sons, Inc. 2022-10-12 2023-02 /pmc/articles/PMC10092822/ /pubmed/36222116 http://dx.doi.org/10.1111/cid.13143 Text en © 2022 The Authors. Clinical Implant Dentistry and Related Research published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Thieu, Minh Khai Le
Stoetzel, Sabine
Rahmati, Maryam
El Khassawna, Thaqif
Verket, Anders
Sanz‐Esporrin, Javier
Sanz, Mariano
Ellingsen, Jan Eirik
Haugen, Håvard Jostein
Immunohistochemical comparison of lateral bone augmentation using a synthetic TiO(2) block or a xenogeneic graft in chronic alveolar defects
title Immunohistochemical comparison of lateral bone augmentation using a synthetic TiO(2) block or a xenogeneic graft in chronic alveolar defects
title_full Immunohistochemical comparison of lateral bone augmentation using a synthetic TiO(2) block or a xenogeneic graft in chronic alveolar defects
title_fullStr Immunohistochemical comparison of lateral bone augmentation using a synthetic TiO(2) block or a xenogeneic graft in chronic alveolar defects
title_full_unstemmed Immunohistochemical comparison of lateral bone augmentation using a synthetic TiO(2) block or a xenogeneic graft in chronic alveolar defects
title_short Immunohistochemical comparison of lateral bone augmentation using a synthetic TiO(2) block or a xenogeneic graft in chronic alveolar defects
title_sort immunohistochemical comparison of lateral bone augmentation using a synthetic tio(2) block or a xenogeneic graft in chronic alveolar defects
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10092822/
https://www.ncbi.nlm.nih.gov/pubmed/36222116
http://dx.doi.org/10.1111/cid.13143
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