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Contamination of Zophobas morio Larvae Rearing Substrate with Listeria monocytogenes: A Preliminary Study
SIMPLE SUMMARY: Over the past 20 years, we have seen exponential growth in the number of scientific publications on edible insects as an alternative food source. Usually, insect larvae are reared in a close environment in constant contact with their feed, called the substrate. The substrates employe...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10092993/ https://www.ncbi.nlm.nih.gov/pubmed/37048454 http://dx.doi.org/10.3390/ani13071198 |
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author | Fratini, Filippo Ciurli, Luca Forzan, Mario Kaboudari, Ata Copelotti, Emma Paci, Gisella Mancini, Simone |
author_facet | Fratini, Filippo Ciurli, Luca Forzan, Mario Kaboudari, Ata Copelotti, Emma Paci, Gisella Mancini, Simone |
author_sort | Fratini, Filippo |
collection | PubMed |
description | SIMPLE SUMMARY: Over the past 20 years, we have seen exponential growth in the number of scientific publications on edible insects as an alternative food source. Usually, insect larvae are reared in a close environment in constant contact with their feed, called the substrate. The substrates employed for rearing could represent one of the most important ways to involuntarily introduce risk in the production chain. In this research, we inoculated one of the most important food pathogens, Listeria monocytogenes, in the substrates used in Zophobas morio rearing (also called superworm). The results highlighted the caution needed in insects rearing and the potential risk related to this pathogen in relation to superworm rearing. Attention was also focused on the technological procedure employed in insect rearing and processing. ABSTRACT: The interest in edible insects is continuously increasing due to their environmental, nutritional, and productive features. The aim of this study was to evaluate the effects and survival of Listeria monocytogenes in Zophobas morio rearing, using two different bacterial loads (2 and 6 log CFU/g). We also considered the effect of washing, fasting, and cooking treatments on the larvae. During the experimental trial, no mortality was observed among the larvae. The L. monocytogenes loads decreased over time, and it was below the detection limit for crates inoculated with 2 log CFU/g, while the substrate inoculated with 6 log CFU/g reached loads of 4.26 (without larvae) and 2.83 log CFU/g (with larvae). Due to the absence of L. monocytogenes in the larvae on day seven, it was not possible to assess the fasting treatment or the washing and the cooking. However, when looking at the total microbial count, significant effects were revealed for all treatments. The unfasted larvae showed no effect of washing, while the total microbial counts decreased after washing in the fasted larvae. This proves that fasting is a good treatment in terms of hygiene assurance for the consumer. |
format | Online Article Text |
id | pubmed-10092993 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100929932023-04-13 Contamination of Zophobas morio Larvae Rearing Substrate with Listeria monocytogenes: A Preliminary Study Fratini, Filippo Ciurli, Luca Forzan, Mario Kaboudari, Ata Copelotti, Emma Paci, Gisella Mancini, Simone Animals (Basel) Communication SIMPLE SUMMARY: Over the past 20 years, we have seen exponential growth in the number of scientific publications on edible insects as an alternative food source. Usually, insect larvae are reared in a close environment in constant contact with their feed, called the substrate. The substrates employed for rearing could represent one of the most important ways to involuntarily introduce risk in the production chain. In this research, we inoculated one of the most important food pathogens, Listeria monocytogenes, in the substrates used in Zophobas morio rearing (also called superworm). The results highlighted the caution needed in insects rearing and the potential risk related to this pathogen in relation to superworm rearing. Attention was also focused on the technological procedure employed in insect rearing and processing. ABSTRACT: The interest in edible insects is continuously increasing due to their environmental, nutritional, and productive features. The aim of this study was to evaluate the effects and survival of Listeria monocytogenes in Zophobas morio rearing, using two different bacterial loads (2 and 6 log CFU/g). We also considered the effect of washing, fasting, and cooking treatments on the larvae. During the experimental trial, no mortality was observed among the larvae. The L. monocytogenes loads decreased over time, and it was below the detection limit for crates inoculated with 2 log CFU/g, while the substrate inoculated with 6 log CFU/g reached loads of 4.26 (without larvae) and 2.83 log CFU/g (with larvae). Due to the absence of L. monocytogenes in the larvae on day seven, it was not possible to assess the fasting treatment or the washing and the cooking. However, when looking at the total microbial count, significant effects were revealed for all treatments. The unfasted larvae showed no effect of washing, while the total microbial counts decreased after washing in the fasted larvae. This proves that fasting is a good treatment in terms of hygiene assurance for the consumer. MDPI 2023-03-29 /pmc/articles/PMC10092993/ /pubmed/37048454 http://dx.doi.org/10.3390/ani13071198 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Fratini, Filippo Ciurli, Luca Forzan, Mario Kaboudari, Ata Copelotti, Emma Paci, Gisella Mancini, Simone Contamination of Zophobas morio Larvae Rearing Substrate with Listeria monocytogenes: A Preliminary Study |
title | Contamination of Zophobas morio Larvae Rearing Substrate with Listeria monocytogenes: A Preliminary Study |
title_full | Contamination of Zophobas morio Larvae Rearing Substrate with Listeria monocytogenes: A Preliminary Study |
title_fullStr | Contamination of Zophobas morio Larvae Rearing Substrate with Listeria monocytogenes: A Preliminary Study |
title_full_unstemmed | Contamination of Zophobas morio Larvae Rearing Substrate with Listeria monocytogenes: A Preliminary Study |
title_short | Contamination of Zophobas morio Larvae Rearing Substrate with Listeria monocytogenes: A Preliminary Study |
title_sort | contamination of zophobas morio larvae rearing substrate with listeria monocytogenes: a preliminary study |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10092993/ https://www.ncbi.nlm.nih.gov/pubmed/37048454 http://dx.doi.org/10.3390/ani13071198 |
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