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Evaluation of the Expression Levels of miR-21-5p and miR-429 Genes in Biopsy Samples from Patients with Oral Squamous Cell Carcinoma

Introduction: MicroRNAs (miRs) are a group of endogenous, non-coding, 18-24 nucleotide length single-strand RNAs that mediate gene expression at the post-transcriptional level through mRNA degradation or translational repression. They are involved in regulating diverse cellular biological processes...

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Autores principales: Garajei, Ata, Allameh, Abdolamir, Azadi, Mehdi, Emami, Azadeh, Atashbasteh, Mostafa, Mostafavi, Melina, Ghaderi, Bayazid, Inchingolo, Francesco, Sadeghi, Masoud, Tadakamadla, Santosh Kumar, Mohammadi, Hady, Tadakamadla, Jyothi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10093007/
https://www.ncbi.nlm.nih.gov/pubmed/37046462
http://dx.doi.org/10.3390/diagnostics13071244
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author Garajei, Ata
Allameh, Abdolamir
Azadi, Mehdi
Emami, Azadeh
Atashbasteh, Mostafa
Mostafavi, Melina
Ghaderi, Bayazid
Inchingolo, Francesco
Sadeghi, Masoud
Tadakamadla, Santosh Kumar
Mohammadi, Hady
Tadakamadla, Jyothi
author_facet Garajei, Ata
Allameh, Abdolamir
Azadi, Mehdi
Emami, Azadeh
Atashbasteh, Mostafa
Mostafavi, Melina
Ghaderi, Bayazid
Inchingolo, Francesco
Sadeghi, Masoud
Tadakamadla, Santosh Kumar
Mohammadi, Hady
Tadakamadla, Jyothi
author_sort Garajei, Ata
collection PubMed
description Introduction: MicroRNAs (miRs) are a group of endogenous, non-coding, 18-24 nucleotide length single-strand RNAs that mediate gene expression at the post-transcriptional level through mRNA degradation or translational repression. They are involved in regulating diverse cellular biological processes such as cell cycle, differentiation, and apoptosis. The deregulation of miRs affects normal biological processes, leading to malignancies, including oral squamous cell carcinoma (OSCC). This study evaluates the expression level of miR-21-5p and miR-429 genes in biopsy samples from patients with OSCC and performs a comparison with controls. Materials and Methods: In this study, tissue samples were obtained from 40 individuals (20 OSCC patients and 20 healthy controls) to determine miR-21-5p and miR-429 expression using the ΔCT method and analyzed by the Mann–Whitney test. Results: The mean age of subjects in the control and patient groups was 47.15 and 53.8 years, respectively. According to the Mann–Whitney test, significant differences were observed in miR-21-5p (p < 0.0001) and miR-429 (p = 0.0191) expression levels between the two groups (p < 0.05). Conclusions: The expression of miR-21-5p, miR-429, and combined miRNAs in the OSCC group was significantly higher compared to the control group. As a result, changes in the expression of these biomarkers in cancerous tissues could potentially be considered as a marker for the early diagnosis of OSCC.
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spelling pubmed-100930072023-04-13 Evaluation of the Expression Levels of miR-21-5p and miR-429 Genes in Biopsy Samples from Patients with Oral Squamous Cell Carcinoma Garajei, Ata Allameh, Abdolamir Azadi, Mehdi Emami, Azadeh Atashbasteh, Mostafa Mostafavi, Melina Ghaderi, Bayazid Inchingolo, Francesco Sadeghi, Masoud Tadakamadla, Santosh Kumar Mohammadi, Hady Tadakamadla, Jyothi Diagnostics (Basel) Article Introduction: MicroRNAs (miRs) are a group of endogenous, non-coding, 18-24 nucleotide length single-strand RNAs that mediate gene expression at the post-transcriptional level through mRNA degradation or translational repression. They are involved in regulating diverse cellular biological processes such as cell cycle, differentiation, and apoptosis. The deregulation of miRs affects normal biological processes, leading to malignancies, including oral squamous cell carcinoma (OSCC). This study evaluates the expression level of miR-21-5p and miR-429 genes in biopsy samples from patients with OSCC and performs a comparison with controls. Materials and Methods: In this study, tissue samples were obtained from 40 individuals (20 OSCC patients and 20 healthy controls) to determine miR-21-5p and miR-429 expression using the ΔCT method and analyzed by the Mann–Whitney test. Results: The mean age of subjects in the control and patient groups was 47.15 and 53.8 years, respectively. According to the Mann–Whitney test, significant differences were observed in miR-21-5p (p < 0.0001) and miR-429 (p = 0.0191) expression levels between the two groups (p < 0.05). Conclusions: The expression of miR-21-5p, miR-429, and combined miRNAs in the OSCC group was significantly higher compared to the control group. As a result, changes in the expression of these biomarkers in cancerous tissues could potentially be considered as a marker for the early diagnosis of OSCC. MDPI 2023-03-25 /pmc/articles/PMC10093007/ /pubmed/37046462 http://dx.doi.org/10.3390/diagnostics13071244 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Garajei, Ata
Allameh, Abdolamir
Azadi, Mehdi
Emami, Azadeh
Atashbasteh, Mostafa
Mostafavi, Melina
Ghaderi, Bayazid
Inchingolo, Francesco
Sadeghi, Masoud
Tadakamadla, Santosh Kumar
Mohammadi, Hady
Tadakamadla, Jyothi
Evaluation of the Expression Levels of miR-21-5p and miR-429 Genes in Biopsy Samples from Patients with Oral Squamous Cell Carcinoma
title Evaluation of the Expression Levels of miR-21-5p and miR-429 Genes in Biopsy Samples from Patients with Oral Squamous Cell Carcinoma
title_full Evaluation of the Expression Levels of miR-21-5p and miR-429 Genes in Biopsy Samples from Patients with Oral Squamous Cell Carcinoma
title_fullStr Evaluation of the Expression Levels of miR-21-5p and miR-429 Genes in Biopsy Samples from Patients with Oral Squamous Cell Carcinoma
title_full_unstemmed Evaluation of the Expression Levels of miR-21-5p and miR-429 Genes in Biopsy Samples from Patients with Oral Squamous Cell Carcinoma
title_short Evaluation of the Expression Levels of miR-21-5p and miR-429 Genes in Biopsy Samples from Patients with Oral Squamous Cell Carcinoma
title_sort evaluation of the expression levels of mir-21-5p and mir-429 genes in biopsy samples from patients with oral squamous cell carcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10093007/
https://www.ncbi.nlm.nih.gov/pubmed/37046462
http://dx.doi.org/10.3390/diagnostics13071244
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