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Oral Vaccination of Largemouth Bass (Micropterus salmoides) against Largemouth Bass Ranavirus (LMBV) Using Yeast Surface Display Technology

SIMPLE SUMMARY: LMBV is an important pathogen in the breeding process of largemouth bass, but there is no effective control method for it. This study developed oral vaccines for LMBV. The vaccines can significantly increase the activity of immune-related enzymes, upregulate the expression of immune...

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Autores principales: Zhang, Mengjie, Chen, Xiaoyu, Xue, Mingyang, Jiang, Nan, Li, Yiqun, Fan, Yuding, Zhang, Peng, Liu, Naicheng, Xiao, Zidong, Zhang, Qinghua, Zhou, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10093309/
https://www.ncbi.nlm.nih.gov/pubmed/37048441
http://dx.doi.org/10.3390/ani13071183
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author Zhang, Mengjie
Chen, Xiaoyu
Xue, Mingyang
Jiang, Nan
Li, Yiqun
Fan, Yuding
Zhang, Peng
Liu, Naicheng
Xiao, Zidong
Zhang, Qinghua
Zhou, Yong
author_facet Zhang, Mengjie
Chen, Xiaoyu
Xue, Mingyang
Jiang, Nan
Li, Yiqun
Fan, Yuding
Zhang, Peng
Liu, Naicheng
Xiao, Zidong
Zhang, Qinghua
Zhou, Yong
author_sort Zhang, Mengjie
collection PubMed
description SIMPLE SUMMARY: LMBV is an important pathogen in the breeding process of largemouth bass, but there is no effective control method for it. This study developed oral vaccines for LMBV. The vaccines can significantly increase the activity of immune-related enzymes, upregulate the expression of immune genes, and stimulate the production of neutralizing antibodies in serum in largemouth bass. In addition, the vaccines can reduce the mortality of LMBV infection. These results indicate that the vaccines are expected to be candidate vaccines for controlling LMBV infection. ABSTRACT: Largemouth bass ranavirus (LMBV) infects largemouth bass, leading to significant mortality and economic losses. There are no safe and effective drugs against this disease. Oral vaccines that directly target the intestinal mucosal immune system play an important role in resisting pathogens. Herein, the B subunit of Escherichia coli heat-labile enterotoxin (LTB, a mucosal immune adjuvant) and the LMBV main capsid protein (MCP) were expressed using Saccharomyces cerevisiae surface display technology. The yeast-prepared oral vaccines were named EBY100-OMCP and EBY100-LTB-OMCP. The candidate vaccines could resist the acidic intestinal environment. After 7 days of continuous oral immunization, indicators of innate and adaptive immunity were measured on days 1, 7, 14, 21, 28, 35, and 42. High activities of immune enzymes (T-SOD, AKP, ACP, and LZM) in serum and intestinal mucus were detected. IgM in the head kidney was significantly upregulated (EBY100-OMCP group: 3.8-fold; BY100-LTB-OMCP group: 4.3-fold). IgT was upregulated in the intestines (EBY100-OMCP group: 5.6-fold; EBY100-LTB-OMCP group: 6.7-fold). Serum neutralizing antibody titers of the two groups reached 1:85. Oral vaccination protected against LMBV infection. The relative percent survival was 52.1% (EBY100-OMCP) and 66.7% (EBY100-LTB-OMCP). Thus, EBY100-OMCP and EBY100-LTB-OMCP are promising and effective candidate vaccines against LMBV infection.
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spelling pubmed-100933092023-04-13 Oral Vaccination of Largemouth Bass (Micropterus salmoides) against Largemouth Bass Ranavirus (LMBV) Using Yeast Surface Display Technology Zhang, Mengjie Chen, Xiaoyu Xue, Mingyang Jiang, Nan Li, Yiqun Fan, Yuding Zhang, Peng Liu, Naicheng Xiao, Zidong Zhang, Qinghua Zhou, Yong Animals (Basel) Article SIMPLE SUMMARY: LMBV is an important pathogen in the breeding process of largemouth bass, but there is no effective control method for it. This study developed oral vaccines for LMBV. The vaccines can significantly increase the activity of immune-related enzymes, upregulate the expression of immune genes, and stimulate the production of neutralizing antibodies in serum in largemouth bass. In addition, the vaccines can reduce the mortality of LMBV infection. These results indicate that the vaccines are expected to be candidate vaccines for controlling LMBV infection. ABSTRACT: Largemouth bass ranavirus (LMBV) infects largemouth bass, leading to significant mortality and economic losses. There are no safe and effective drugs against this disease. Oral vaccines that directly target the intestinal mucosal immune system play an important role in resisting pathogens. Herein, the B subunit of Escherichia coli heat-labile enterotoxin (LTB, a mucosal immune adjuvant) and the LMBV main capsid protein (MCP) were expressed using Saccharomyces cerevisiae surface display technology. The yeast-prepared oral vaccines were named EBY100-OMCP and EBY100-LTB-OMCP. The candidate vaccines could resist the acidic intestinal environment. After 7 days of continuous oral immunization, indicators of innate and adaptive immunity were measured on days 1, 7, 14, 21, 28, 35, and 42. High activities of immune enzymes (T-SOD, AKP, ACP, and LZM) in serum and intestinal mucus were detected. IgM in the head kidney was significantly upregulated (EBY100-OMCP group: 3.8-fold; BY100-LTB-OMCP group: 4.3-fold). IgT was upregulated in the intestines (EBY100-OMCP group: 5.6-fold; EBY100-LTB-OMCP group: 6.7-fold). Serum neutralizing antibody titers of the two groups reached 1:85. Oral vaccination protected against LMBV infection. The relative percent survival was 52.1% (EBY100-OMCP) and 66.7% (EBY100-LTB-OMCP). Thus, EBY100-OMCP and EBY100-LTB-OMCP are promising and effective candidate vaccines against LMBV infection. MDPI 2023-03-28 /pmc/articles/PMC10093309/ /pubmed/37048441 http://dx.doi.org/10.3390/ani13071183 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Mengjie
Chen, Xiaoyu
Xue, Mingyang
Jiang, Nan
Li, Yiqun
Fan, Yuding
Zhang, Peng
Liu, Naicheng
Xiao, Zidong
Zhang, Qinghua
Zhou, Yong
Oral Vaccination of Largemouth Bass (Micropterus salmoides) against Largemouth Bass Ranavirus (LMBV) Using Yeast Surface Display Technology
title Oral Vaccination of Largemouth Bass (Micropterus salmoides) against Largemouth Bass Ranavirus (LMBV) Using Yeast Surface Display Technology
title_full Oral Vaccination of Largemouth Bass (Micropterus salmoides) against Largemouth Bass Ranavirus (LMBV) Using Yeast Surface Display Technology
title_fullStr Oral Vaccination of Largemouth Bass (Micropterus salmoides) against Largemouth Bass Ranavirus (LMBV) Using Yeast Surface Display Technology
title_full_unstemmed Oral Vaccination of Largemouth Bass (Micropterus salmoides) against Largemouth Bass Ranavirus (LMBV) Using Yeast Surface Display Technology
title_short Oral Vaccination of Largemouth Bass (Micropterus salmoides) against Largemouth Bass Ranavirus (LMBV) Using Yeast Surface Display Technology
title_sort oral vaccination of largemouth bass (micropterus salmoides) against largemouth bass ranavirus (lmbv) using yeast surface display technology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10093309/
https://www.ncbi.nlm.nih.gov/pubmed/37048441
http://dx.doi.org/10.3390/ani13071183
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