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miR-139-5p and miR-451a as a Diagnostic Biomarker in LUSC
BACKGROUND: Lung squamous cell carcinoma (LUSC) is a type of lung cancer that originates from segmental or subsegmental bronchial mucosa. There is evidence that miRNA plays an important role in the occurrence and progression of tumors. METHODS: In this study, plasma samples of patients with early LU...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Dove
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10093518/ https://www.ncbi.nlm.nih.gov/pubmed/37063774 http://dx.doi.org/10.2147/PGPM.S402750 |
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author | Gao, Bo Li, Rui Song, Xiaojia Hu, Shan Yang, Fengmei |
author_facet | Gao, Bo Li, Rui Song, Xiaojia Hu, Shan Yang, Fengmei |
author_sort | Gao, Bo |
collection | PubMed |
description | BACKGROUND: Lung squamous cell carcinoma (LUSC) is a type of lung cancer that originates from segmental or subsegmental bronchial mucosa. There is evidence that miRNA plays an important role in the occurrence and progression of tumors. METHODS: In this study, plasma samples of patients with early LUSC and healthy volunteers were subjected to miRNA sequencing, and the levels of differentially expressed miRNAs (DEMs) in LUSC tissues were analyzed using R language. Cox regression and Kaplan–Meier (K-M) survival curve analyses were performed to determine the relationship between DEMs and prognosis in LUSC, and PCR method was verified for the plasma expression level of DEMs in patients with LUSC. The levels of CYFRA21-1 and SCC-Ag in plasma were measured, and area under curve (AUC) was used to evaluate the diagnostic value of the DEMs. RESULTS: A total of 21 DEMs were screened out by sequencing. The expression levels of DEMs in tissue samples in the TCGA database were analyzed, and four DEMs with consistent expression levels were further screened from plasma and tissue samples. Regression analysis and K-M curve were performed to select two DEMs (miR-139-5p, miR-451a) that were correlated with the prognosis. PCR verification results showed that the levels of miR-451a and miR-139-5p were low in patients, and the level of miR-139-5p in late stages III & IV with the patients of LUSC was higher than that in stages I & II. The AUC values of the four indicators (SCC-Ag, CYFRA21-1, miR-451a and miR-139-5p) in the diagnosis of LUSC, early and late cases were 0.884, 0.935 and 0.778, respectively. CONCLUSION: The detection of miR-139-5p and miR-451a levels in plasma has a certain potential in the non-invasive diagnosis, especially in patients with early stages of LUSC. |
format | Online Article Text |
id | pubmed-10093518 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-100935182023-04-13 miR-139-5p and miR-451a as a Diagnostic Biomarker in LUSC Gao, Bo Li, Rui Song, Xiaojia Hu, Shan Yang, Fengmei Pharmgenomics Pers Med Original Research BACKGROUND: Lung squamous cell carcinoma (LUSC) is a type of lung cancer that originates from segmental or subsegmental bronchial mucosa. There is evidence that miRNA plays an important role in the occurrence and progression of tumors. METHODS: In this study, plasma samples of patients with early LUSC and healthy volunteers were subjected to miRNA sequencing, and the levels of differentially expressed miRNAs (DEMs) in LUSC tissues were analyzed using R language. Cox regression and Kaplan–Meier (K-M) survival curve analyses were performed to determine the relationship between DEMs and prognosis in LUSC, and PCR method was verified for the plasma expression level of DEMs in patients with LUSC. The levels of CYFRA21-1 and SCC-Ag in plasma were measured, and area under curve (AUC) was used to evaluate the diagnostic value of the DEMs. RESULTS: A total of 21 DEMs were screened out by sequencing. The expression levels of DEMs in tissue samples in the TCGA database were analyzed, and four DEMs with consistent expression levels were further screened from plasma and tissue samples. Regression analysis and K-M curve were performed to select two DEMs (miR-139-5p, miR-451a) that were correlated with the prognosis. PCR verification results showed that the levels of miR-451a and miR-139-5p were low in patients, and the level of miR-139-5p in late stages III & IV with the patients of LUSC was higher than that in stages I & II. The AUC values of the four indicators (SCC-Ag, CYFRA21-1, miR-451a and miR-139-5p) in the diagnosis of LUSC, early and late cases were 0.884, 0.935 and 0.778, respectively. CONCLUSION: The detection of miR-139-5p and miR-451a levels in plasma has a certain potential in the non-invasive diagnosis, especially in patients with early stages of LUSC. Dove 2023-04-08 /pmc/articles/PMC10093518/ /pubmed/37063774 http://dx.doi.org/10.2147/PGPM.S402750 Text en © 2023 Gao et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Gao, Bo Li, Rui Song, Xiaojia Hu, Shan Yang, Fengmei miR-139-5p and miR-451a as a Diagnostic Biomarker in LUSC |
title | miR-139-5p and miR-451a as a Diagnostic Biomarker in LUSC |
title_full | miR-139-5p and miR-451a as a Diagnostic Biomarker in LUSC |
title_fullStr | miR-139-5p and miR-451a as a Diagnostic Biomarker in LUSC |
title_full_unstemmed | miR-139-5p and miR-451a as a Diagnostic Biomarker in LUSC |
title_short | miR-139-5p and miR-451a as a Diagnostic Biomarker in LUSC |
title_sort | mir-139-5p and mir-451a as a diagnostic biomarker in lusc |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10093518/ https://www.ncbi.nlm.nih.gov/pubmed/37063774 http://dx.doi.org/10.2147/PGPM.S402750 |
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