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Primary Cilium Identifies a Quiescent Cell Population in the Human Intestinal Crypt

Primary cilia are sensory antennae located at the cell surface which mediate a variety of extracellular signals involved in development, tissue homeostasis, stem cells and cancer. Primary cilia are found in an extensive array of vertebrae cells but can only be generated when cells become quiescent....

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Autores principales: Sénicourt, Blanche, Cloutier, Gabriel, Basora, Nuria, Fallah, Sepideh, Laniel, Andréanne, Lavoie, Christine, Beaulieu, Jean-François
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10093653/
https://www.ncbi.nlm.nih.gov/pubmed/37048132
http://dx.doi.org/10.3390/cells12071059
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author Sénicourt, Blanche
Cloutier, Gabriel
Basora, Nuria
Fallah, Sepideh
Laniel, Andréanne
Lavoie, Christine
Beaulieu, Jean-François
author_facet Sénicourt, Blanche
Cloutier, Gabriel
Basora, Nuria
Fallah, Sepideh
Laniel, Andréanne
Lavoie, Christine
Beaulieu, Jean-François
author_sort Sénicourt, Blanche
collection PubMed
description Primary cilia are sensory antennae located at the cell surface which mediate a variety of extracellular signals involved in development, tissue homeostasis, stem cells and cancer. Primary cilia are found in an extensive array of vertebrae cells but can only be generated when cells become quiescent. The small intestinal epithelium is a rapidly self-renewing tissue organized into a functional unit called the crypt–villus axis, containing progenitor and differentiated cells, respectively. Terminally differentiated villus cells are notoriously devoid of primary cilia. We sought to determine if intestinal crypts contain a quiescent cell population that could be identified by the presence of primary cilia. Here we show that primary cilia are detected in a subset of cells located deep in the crypts slightly above a Paneth cell population. Using a normal epithelial proliferative crypt cell model, we show that primary cilia assembly and activity correlate with a quiescent state. These results provide further evidence for the existence of a quiescent cell population in the human small intestine and suggest the potential for new modes of regulation in stem cell dynamics.
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spelling pubmed-100936532023-04-13 Primary Cilium Identifies a Quiescent Cell Population in the Human Intestinal Crypt Sénicourt, Blanche Cloutier, Gabriel Basora, Nuria Fallah, Sepideh Laniel, Andréanne Lavoie, Christine Beaulieu, Jean-François Cells Article Primary cilia are sensory antennae located at the cell surface which mediate a variety of extracellular signals involved in development, tissue homeostasis, stem cells and cancer. Primary cilia are found in an extensive array of vertebrae cells but can only be generated when cells become quiescent. The small intestinal epithelium is a rapidly self-renewing tissue organized into a functional unit called the crypt–villus axis, containing progenitor and differentiated cells, respectively. Terminally differentiated villus cells are notoriously devoid of primary cilia. We sought to determine if intestinal crypts contain a quiescent cell population that could be identified by the presence of primary cilia. Here we show that primary cilia are detected in a subset of cells located deep in the crypts slightly above a Paneth cell population. Using a normal epithelial proliferative crypt cell model, we show that primary cilia assembly and activity correlate with a quiescent state. These results provide further evidence for the existence of a quiescent cell population in the human small intestine and suggest the potential for new modes of regulation in stem cell dynamics. MDPI 2023-03-31 /pmc/articles/PMC10093653/ /pubmed/37048132 http://dx.doi.org/10.3390/cells12071059 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sénicourt, Blanche
Cloutier, Gabriel
Basora, Nuria
Fallah, Sepideh
Laniel, Andréanne
Lavoie, Christine
Beaulieu, Jean-François
Primary Cilium Identifies a Quiescent Cell Population in the Human Intestinal Crypt
title Primary Cilium Identifies a Quiescent Cell Population in the Human Intestinal Crypt
title_full Primary Cilium Identifies a Quiescent Cell Population in the Human Intestinal Crypt
title_fullStr Primary Cilium Identifies a Quiescent Cell Population in the Human Intestinal Crypt
title_full_unstemmed Primary Cilium Identifies a Quiescent Cell Population in the Human Intestinal Crypt
title_short Primary Cilium Identifies a Quiescent Cell Population in the Human Intestinal Crypt
title_sort primary cilium identifies a quiescent cell population in the human intestinal crypt
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10093653/
https://www.ncbi.nlm.nih.gov/pubmed/37048132
http://dx.doi.org/10.3390/cells12071059
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