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Primary Cilium Identifies a Quiescent Cell Population in the Human Intestinal Crypt
Primary cilia are sensory antennae located at the cell surface which mediate a variety of extracellular signals involved in development, tissue homeostasis, stem cells and cancer. Primary cilia are found in an extensive array of vertebrae cells but can only be generated when cells become quiescent....
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10093653/ https://www.ncbi.nlm.nih.gov/pubmed/37048132 http://dx.doi.org/10.3390/cells12071059 |
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author | Sénicourt, Blanche Cloutier, Gabriel Basora, Nuria Fallah, Sepideh Laniel, Andréanne Lavoie, Christine Beaulieu, Jean-François |
author_facet | Sénicourt, Blanche Cloutier, Gabriel Basora, Nuria Fallah, Sepideh Laniel, Andréanne Lavoie, Christine Beaulieu, Jean-François |
author_sort | Sénicourt, Blanche |
collection | PubMed |
description | Primary cilia are sensory antennae located at the cell surface which mediate a variety of extracellular signals involved in development, tissue homeostasis, stem cells and cancer. Primary cilia are found in an extensive array of vertebrae cells but can only be generated when cells become quiescent. The small intestinal epithelium is a rapidly self-renewing tissue organized into a functional unit called the crypt–villus axis, containing progenitor and differentiated cells, respectively. Terminally differentiated villus cells are notoriously devoid of primary cilia. We sought to determine if intestinal crypts contain a quiescent cell population that could be identified by the presence of primary cilia. Here we show that primary cilia are detected in a subset of cells located deep in the crypts slightly above a Paneth cell population. Using a normal epithelial proliferative crypt cell model, we show that primary cilia assembly and activity correlate with a quiescent state. These results provide further evidence for the existence of a quiescent cell population in the human small intestine and suggest the potential for new modes of regulation in stem cell dynamics. |
format | Online Article Text |
id | pubmed-10093653 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100936532023-04-13 Primary Cilium Identifies a Quiescent Cell Population in the Human Intestinal Crypt Sénicourt, Blanche Cloutier, Gabriel Basora, Nuria Fallah, Sepideh Laniel, Andréanne Lavoie, Christine Beaulieu, Jean-François Cells Article Primary cilia are sensory antennae located at the cell surface which mediate a variety of extracellular signals involved in development, tissue homeostasis, stem cells and cancer. Primary cilia are found in an extensive array of vertebrae cells but can only be generated when cells become quiescent. The small intestinal epithelium is a rapidly self-renewing tissue organized into a functional unit called the crypt–villus axis, containing progenitor and differentiated cells, respectively. Terminally differentiated villus cells are notoriously devoid of primary cilia. We sought to determine if intestinal crypts contain a quiescent cell population that could be identified by the presence of primary cilia. Here we show that primary cilia are detected in a subset of cells located deep in the crypts slightly above a Paneth cell population. Using a normal epithelial proliferative crypt cell model, we show that primary cilia assembly and activity correlate with a quiescent state. These results provide further evidence for the existence of a quiescent cell population in the human small intestine and suggest the potential for new modes of regulation in stem cell dynamics. MDPI 2023-03-31 /pmc/articles/PMC10093653/ /pubmed/37048132 http://dx.doi.org/10.3390/cells12071059 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sénicourt, Blanche Cloutier, Gabriel Basora, Nuria Fallah, Sepideh Laniel, Andréanne Lavoie, Christine Beaulieu, Jean-François Primary Cilium Identifies a Quiescent Cell Population in the Human Intestinal Crypt |
title | Primary Cilium Identifies a Quiescent Cell Population in the Human Intestinal Crypt |
title_full | Primary Cilium Identifies a Quiescent Cell Population in the Human Intestinal Crypt |
title_fullStr | Primary Cilium Identifies a Quiescent Cell Population in the Human Intestinal Crypt |
title_full_unstemmed | Primary Cilium Identifies a Quiescent Cell Population in the Human Intestinal Crypt |
title_short | Primary Cilium Identifies a Quiescent Cell Population in the Human Intestinal Crypt |
title_sort | primary cilium identifies a quiescent cell population in the human intestinal crypt |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10093653/ https://www.ncbi.nlm.nih.gov/pubmed/37048132 http://dx.doi.org/10.3390/cells12071059 |
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