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The Alterations of Serum N-glycome in Response to SARS-CoV-2 Vaccination
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has caused a global concern since its outbreak in 2019, with one of the main solutions being vaccination. Altered glycosylation has been described in patients after SARS-CoV-2 infection, while the effect of vaccination on seru...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10093923/ https://www.ncbi.nlm.nih.gov/pubmed/37047177 http://dx.doi.org/10.3390/ijms24076203 |
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author | Dojcsák, Dalma Kardos, Zsófia Szabó, Miklós Oláh, Csaba Körömi, Zsolt Viskolcz, Béla Váradi, Csaba |
author_facet | Dojcsák, Dalma Kardos, Zsófia Szabó, Miklós Oláh, Csaba Körömi, Zsolt Viskolcz, Béla Váradi, Csaba |
author_sort | Dojcsák, Dalma |
collection | PubMed |
description | The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has caused a global concern since its outbreak in 2019, with one of the main solutions being vaccination. Altered glycosylation has been described in patients after SARS-CoV-2 infection, while the effect of vaccination on serum glycoproteins remained unexplored. In this study, total serum glycosylation was analyzed in patients after SARS-CoV-2 infection and/or mRNA vaccination in order to identify potential glycosylation-based alterations. Enzyme-linked immunosorbent assay was applied to identify post-COVID-19 and post-Vaccinated patients and rule out potential outliers. Serum samples were deglycosylated by PNGase F digestion, and the released glycans were fluorescently derivatized using procainamide labeling. Solid-phase extraction was used to purify the labeled glycans followed by the analysis of hydrophilic-interaction liquid chromatography with fluorescence and mass-spectrometric detection. Alterations of serum N-glycome in response to SARS-CoV-2 infection and mRNA vaccination were revealed by linear discriminant analysis. |
format | Online Article Text |
id | pubmed-10093923 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100939232023-04-13 The Alterations of Serum N-glycome in Response to SARS-CoV-2 Vaccination Dojcsák, Dalma Kardos, Zsófia Szabó, Miklós Oláh, Csaba Körömi, Zsolt Viskolcz, Béla Váradi, Csaba Int J Mol Sci Communication The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has caused a global concern since its outbreak in 2019, with one of the main solutions being vaccination. Altered glycosylation has been described in patients after SARS-CoV-2 infection, while the effect of vaccination on serum glycoproteins remained unexplored. In this study, total serum glycosylation was analyzed in patients after SARS-CoV-2 infection and/or mRNA vaccination in order to identify potential glycosylation-based alterations. Enzyme-linked immunosorbent assay was applied to identify post-COVID-19 and post-Vaccinated patients and rule out potential outliers. Serum samples were deglycosylated by PNGase F digestion, and the released glycans were fluorescently derivatized using procainamide labeling. Solid-phase extraction was used to purify the labeled glycans followed by the analysis of hydrophilic-interaction liquid chromatography with fluorescence and mass-spectrometric detection. Alterations of serum N-glycome in response to SARS-CoV-2 infection and mRNA vaccination were revealed by linear discriminant analysis. MDPI 2023-03-25 /pmc/articles/PMC10093923/ /pubmed/37047177 http://dx.doi.org/10.3390/ijms24076203 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Dojcsák, Dalma Kardos, Zsófia Szabó, Miklós Oláh, Csaba Körömi, Zsolt Viskolcz, Béla Váradi, Csaba The Alterations of Serum N-glycome in Response to SARS-CoV-2 Vaccination |
title | The Alterations of Serum N-glycome in Response to SARS-CoV-2 Vaccination |
title_full | The Alterations of Serum N-glycome in Response to SARS-CoV-2 Vaccination |
title_fullStr | The Alterations of Serum N-glycome in Response to SARS-CoV-2 Vaccination |
title_full_unstemmed | The Alterations of Serum N-glycome in Response to SARS-CoV-2 Vaccination |
title_short | The Alterations of Serum N-glycome in Response to SARS-CoV-2 Vaccination |
title_sort | alterations of serum n-glycome in response to sars-cov-2 vaccination |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10093923/ https://www.ncbi.nlm.nih.gov/pubmed/37047177 http://dx.doi.org/10.3390/ijms24076203 |
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