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Generating Functional and Highly Proliferative Melanocytes Derived from Human Pluripotent Stem Cells: A Promising Tool for Biotherapeutic Approaches to Treat Skin Pigmentation Disorders

Melanocytes are essential for skin homeostasis and protection, and their loss or misfunction leads to a wide spectrum of diseases. Cell therapy utilizing autologous melanocytes has been used for years as an adjunct treatment for hypopigmentary disorders such as vitiligo. However, these approaches ar...

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Autores principales: Saidani, Manoubia, Darle, Annabelle, Jarrige, Margot, Polveche, Hélène, El Kassar, Lina, Julié, Séverine, Bessou-Touya, Sandrine, Holic, Nathalie, Lemaitre, Gilles, Martinat, Cécile, Baldeschi, Christine, Allouche, Jennifer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094141/
https://www.ncbi.nlm.nih.gov/pubmed/37047372
http://dx.doi.org/10.3390/ijms24076398
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author Saidani, Manoubia
Darle, Annabelle
Jarrige, Margot
Polveche, Hélène
El Kassar, Lina
Julié, Séverine
Bessou-Touya, Sandrine
Holic, Nathalie
Lemaitre, Gilles
Martinat, Cécile
Baldeschi, Christine
Allouche, Jennifer
author_facet Saidani, Manoubia
Darle, Annabelle
Jarrige, Margot
Polveche, Hélène
El Kassar, Lina
Julié, Séverine
Bessou-Touya, Sandrine
Holic, Nathalie
Lemaitre, Gilles
Martinat, Cécile
Baldeschi, Christine
Allouche, Jennifer
author_sort Saidani, Manoubia
collection PubMed
description Melanocytes are essential for skin homeostasis and protection, and their loss or misfunction leads to a wide spectrum of diseases. Cell therapy utilizing autologous melanocytes has been used for years as an adjunct treatment for hypopigmentary disorders such as vitiligo. However, these approaches are hindered by the poor proliferative capacity of melanocytes obtained from skin biopsies. Recent advances in the field of human pluripotent stem cells have fueled the prospect of generating melanocytes. Here, we have developed a well-characterized method to produce a pure and homogenous population of functional and proliferative melanocytes. The genetic stability and potential transformation of melanocytes from pluripotent stem cells have been evaluated over time during the in vitro culture process. Thanks to transcriptomic analysis, the molecular signatures all along the differentiation protocol have been characterized, providing a solid basis for standardizing the protocol. Altogether, our results promise meaningful, broadly applicable, and longer-lasting advances for pigmentation disorders and open perspectives for innovative biotherapies for pigment disorders.
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spelling pubmed-100941412023-04-13 Generating Functional and Highly Proliferative Melanocytes Derived from Human Pluripotent Stem Cells: A Promising Tool for Biotherapeutic Approaches to Treat Skin Pigmentation Disorders Saidani, Manoubia Darle, Annabelle Jarrige, Margot Polveche, Hélène El Kassar, Lina Julié, Séverine Bessou-Touya, Sandrine Holic, Nathalie Lemaitre, Gilles Martinat, Cécile Baldeschi, Christine Allouche, Jennifer Int J Mol Sci Article Melanocytes are essential for skin homeostasis and protection, and their loss or misfunction leads to a wide spectrum of diseases. Cell therapy utilizing autologous melanocytes has been used for years as an adjunct treatment for hypopigmentary disorders such as vitiligo. However, these approaches are hindered by the poor proliferative capacity of melanocytes obtained from skin biopsies. Recent advances in the field of human pluripotent stem cells have fueled the prospect of generating melanocytes. Here, we have developed a well-characterized method to produce a pure and homogenous population of functional and proliferative melanocytes. The genetic stability and potential transformation of melanocytes from pluripotent stem cells have been evaluated over time during the in vitro culture process. Thanks to transcriptomic analysis, the molecular signatures all along the differentiation protocol have been characterized, providing a solid basis for standardizing the protocol. Altogether, our results promise meaningful, broadly applicable, and longer-lasting advances for pigmentation disorders and open perspectives for innovative biotherapies for pigment disorders. MDPI 2023-03-29 /pmc/articles/PMC10094141/ /pubmed/37047372 http://dx.doi.org/10.3390/ijms24076398 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Saidani, Manoubia
Darle, Annabelle
Jarrige, Margot
Polveche, Hélène
El Kassar, Lina
Julié, Séverine
Bessou-Touya, Sandrine
Holic, Nathalie
Lemaitre, Gilles
Martinat, Cécile
Baldeschi, Christine
Allouche, Jennifer
Generating Functional and Highly Proliferative Melanocytes Derived from Human Pluripotent Stem Cells: A Promising Tool for Biotherapeutic Approaches to Treat Skin Pigmentation Disorders
title Generating Functional and Highly Proliferative Melanocytes Derived from Human Pluripotent Stem Cells: A Promising Tool for Biotherapeutic Approaches to Treat Skin Pigmentation Disorders
title_full Generating Functional and Highly Proliferative Melanocytes Derived from Human Pluripotent Stem Cells: A Promising Tool for Biotherapeutic Approaches to Treat Skin Pigmentation Disorders
title_fullStr Generating Functional and Highly Proliferative Melanocytes Derived from Human Pluripotent Stem Cells: A Promising Tool for Biotherapeutic Approaches to Treat Skin Pigmentation Disorders
title_full_unstemmed Generating Functional and Highly Proliferative Melanocytes Derived from Human Pluripotent Stem Cells: A Promising Tool for Biotherapeutic Approaches to Treat Skin Pigmentation Disorders
title_short Generating Functional and Highly Proliferative Melanocytes Derived from Human Pluripotent Stem Cells: A Promising Tool for Biotherapeutic Approaches to Treat Skin Pigmentation Disorders
title_sort generating functional and highly proliferative melanocytes derived from human pluripotent stem cells: a promising tool for biotherapeutic approaches to treat skin pigmentation disorders
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094141/
https://www.ncbi.nlm.nih.gov/pubmed/37047372
http://dx.doi.org/10.3390/ijms24076398
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