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Generating Functional and Highly Proliferative Melanocytes Derived from Human Pluripotent Stem Cells: A Promising Tool for Biotherapeutic Approaches to Treat Skin Pigmentation Disorders
Melanocytes are essential for skin homeostasis and protection, and their loss or misfunction leads to a wide spectrum of diseases. Cell therapy utilizing autologous melanocytes has been used for years as an adjunct treatment for hypopigmentary disorders such as vitiligo. However, these approaches ar...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094141/ https://www.ncbi.nlm.nih.gov/pubmed/37047372 http://dx.doi.org/10.3390/ijms24076398 |
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author | Saidani, Manoubia Darle, Annabelle Jarrige, Margot Polveche, Hélène El Kassar, Lina Julié, Séverine Bessou-Touya, Sandrine Holic, Nathalie Lemaitre, Gilles Martinat, Cécile Baldeschi, Christine Allouche, Jennifer |
author_facet | Saidani, Manoubia Darle, Annabelle Jarrige, Margot Polveche, Hélène El Kassar, Lina Julié, Séverine Bessou-Touya, Sandrine Holic, Nathalie Lemaitre, Gilles Martinat, Cécile Baldeschi, Christine Allouche, Jennifer |
author_sort | Saidani, Manoubia |
collection | PubMed |
description | Melanocytes are essential for skin homeostasis and protection, and their loss or misfunction leads to a wide spectrum of diseases. Cell therapy utilizing autologous melanocytes has been used for years as an adjunct treatment for hypopigmentary disorders such as vitiligo. However, these approaches are hindered by the poor proliferative capacity of melanocytes obtained from skin biopsies. Recent advances in the field of human pluripotent stem cells have fueled the prospect of generating melanocytes. Here, we have developed a well-characterized method to produce a pure and homogenous population of functional and proliferative melanocytes. The genetic stability and potential transformation of melanocytes from pluripotent stem cells have been evaluated over time during the in vitro culture process. Thanks to transcriptomic analysis, the molecular signatures all along the differentiation protocol have been characterized, providing a solid basis for standardizing the protocol. Altogether, our results promise meaningful, broadly applicable, and longer-lasting advances for pigmentation disorders and open perspectives for innovative biotherapies for pigment disorders. |
format | Online Article Text |
id | pubmed-10094141 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100941412023-04-13 Generating Functional and Highly Proliferative Melanocytes Derived from Human Pluripotent Stem Cells: A Promising Tool for Biotherapeutic Approaches to Treat Skin Pigmentation Disorders Saidani, Manoubia Darle, Annabelle Jarrige, Margot Polveche, Hélène El Kassar, Lina Julié, Séverine Bessou-Touya, Sandrine Holic, Nathalie Lemaitre, Gilles Martinat, Cécile Baldeschi, Christine Allouche, Jennifer Int J Mol Sci Article Melanocytes are essential for skin homeostasis and protection, and their loss or misfunction leads to a wide spectrum of diseases. Cell therapy utilizing autologous melanocytes has been used for years as an adjunct treatment for hypopigmentary disorders such as vitiligo. However, these approaches are hindered by the poor proliferative capacity of melanocytes obtained from skin biopsies. Recent advances in the field of human pluripotent stem cells have fueled the prospect of generating melanocytes. Here, we have developed a well-characterized method to produce a pure and homogenous population of functional and proliferative melanocytes. The genetic stability and potential transformation of melanocytes from pluripotent stem cells have been evaluated over time during the in vitro culture process. Thanks to transcriptomic analysis, the molecular signatures all along the differentiation protocol have been characterized, providing a solid basis for standardizing the protocol. Altogether, our results promise meaningful, broadly applicable, and longer-lasting advances for pigmentation disorders and open perspectives for innovative biotherapies for pigment disorders. MDPI 2023-03-29 /pmc/articles/PMC10094141/ /pubmed/37047372 http://dx.doi.org/10.3390/ijms24076398 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Saidani, Manoubia Darle, Annabelle Jarrige, Margot Polveche, Hélène El Kassar, Lina Julié, Séverine Bessou-Touya, Sandrine Holic, Nathalie Lemaitre, Gilles Martinat, Cécile Baldeschi, Christine Allouche, Jennifer Generating Functional and Highly Proliferative Melanocytes Derived from Human Pluripotent Stem Cells: A Promising Tool for Biotherapeutic Approaches to Treat Skin Pigmentation Disorders |
title | Generating Functional and Highly Proliferative Melanocytes Derived from Human Pluripotent Stem Cells: A Promising Tool for Biotherapeutic Approaches to Treat Skin Pigmentation Disorders |
title_full | Generating Functional and Highly Proliferative Melanocytes Derived from Human Pluripotent Stem Cells: A Promising Tool for Biotherapeutic Approaches to Treat Skin Pigmentation Disorders |
title_fullStr | Generating Functional and Highly Proliferative Melanocytes Derived from Human Pluripotent Stem Cells: A Promising Tool for Biotherapeutic Approaches to Treat Skin Pigmentation Disorders |
title_full_unstemmed | Generating Functional and Highly Proliferative Melanocytes Derived from Human Pluripotent Stem Cells: A Promising Tool for Biotherapeutic Approaches to Treat Skin Pigmentation Disorders |
title_short | Generating Functional and Highly Proliferative Melanocytes Derived from Human Pluripotent Stem Cells: A Promising Tool for Biotherapeutic Approaches to Treat Skin Pigmentation Disorders |
title_sort | generating functional and highly proliferative melanocytes derived from human pluripotent stem cells: a promising tool for biotherapeutic approaches to treat skin pigmentation disorders |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094141/ https://www.ncbi.nlm.nih.gov/pubmed/37047372 http://dx.doi.org/10.3390/ijms24076398 |
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