Cargando…
Identification and Functional Characterization of CsMYCs in Cucumber Glandular Trichome Development
Glandular trichomes (GTs), specialized structures formed by the differentiation of plant epidermal cells, are known to play important roles in the resistance of plants to external biotic and abiotic stresses. These structures are capable of storing and secreting secondary metabolites, which often ha...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094329/ https://www.ncbi.nlm.nih.gov/pubmed/37047408 http://dx.doi.org/10.3390/ijms24076435 |
_version_ | 1785023815153090560 |
---|---|
author | Feng, Zhongxuan Sun, Lei Dong, Mingming Fan, Shanshan Shi, Kexin Qu, Yixin Zhu, Liyan Shi, Jinfeng Wang, Wujun Liu, Yihan Chen, Xiaofeng Weng, Yiqun Liu, Xingwang Ren, Huazhong |
author_facet | Feng, Zhongxuan Sun, Lei Dong, Mingming Fan, Shanshan Shi, Kexin Qu, Yixin Zhu, Liyan Shi, Jinfeng Wang, Wujun Liu, Yihan Chen, Xiaofeng Weng, Yiqun Liu, Xingwang Ren, Huazhong |
author_sort | Feng, Zhongxuan |
collection | PubMed |
description | Glandular trichomes (GTs), specialized structures formed by the differentiation of plant epidermal cells, are known to play important roles in the resistance of plants to external biotic and abiotic stresses. These structures are capable of storing and secreting secondary metabolites, which often have important agricultural and medicinal values. In order to better understand the molecular developmental mechanisms of GTs, studies have been conducted in a variety of crops, including tomato (Solanum lycopersicum), sweetworm (Artemisia annua), and cotton (Gossypium hirsutum). The MYC transcription factor of the basic helix-loop-helix (bHLH) transcription factor family has been found to play an important role in GT development. In this study, a total of 13 cucumber MYC transcription factors were identified in the cucumber (Cucumis sativus L.) genome. After performing phylogenetic analyses and conserved motifs on the 13 CsMYCs in comparison to previously reported MYC transcription factors that regulate trichome development, seven candidate MYC transcription factors were selected. Through virus-induced gene silencing (VIGS), CsMYC2 is found to negatively regulate GT formation while CsMYC4, CsMYC5, CsMYC6, CsMYC7, and CsMYC8 are found to positively regulate GT formation. Furthermore, the two master effector genes, CsMYC2 and CsMYC7, are observed to have similar expression patterns indicating that they co-regulate the balance of GT development in an antagonistic way. |
format | Online Article Text |
id | pubmed-10094329 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100943292023-04-13 Identification and Functional Characterization of CsMYCs in Cucumber Glandular Trichome Development Feng, Zhongxuan Sun, Lei Dong, Mingming Fan, Shanshan Shi, Kexin Qu, Yixin Zhu, Liyan Shi, Jinfeng Wang, Wujun Liu, Yihan Chen, Xiaofeng Weng, Yiqun Liu, Xingwang Ren, Huazhong Int J Mol Sci Article Glandular trichomes (GTs), specialized structures formed by the differentiation of plant epidermal cells, are known to play important roles in the resistance of plants to external biotic and abiotic stresses. These structures are capable of storing and secreting secondary metabolites, which often have important agricultural and medicinal values. In order to better understand the molecular developmental mechanisms of GTs, studies have been conducted in a variety of crops, including tomato (Solanum lycopersicum), sweetworm (Artemisia annua), and cotton (Gossypium hirsutum). The MYC transcription factor of the basic helix-loop-helix (bHLH) transcription factor family has been found to play an important role in GT development. In this study, a total of 13 cucumber MYC transcription factors were identified in the cucumber (Cucumis sativus L.) genome. After performing phylogenetic analyses and conserved motifs on the 13 CsMYCs in comparison to previously reported MYC transcription factors that regulate trichome development, seven candidate MYC transcription factors were selected. Through virus-induced gene silencing (VIGS), CsMYC2 is found to negatively regulate GT formation while CsMYC4, CsMYC5, CsMYC6, CsMYC7, and CsMYC8 are found to positively regulate GT formation. Furthermore, the two master effector genes, CsMYC2 and CsMYC7, are observed to have similar expression patterns indicating that they co-regulate the balance of GT development in an antagonistic way. MDPI 2023-03-29 /pmc/articles/PMC10094329/ /pubmed/37047408 http://dx.doi.org/10.3390/ijms24076435 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Feng, Zhongxuan Sun, Lei Dong, Mingming Fan, Shanshan Shi, Kexin Qu, Yixin Zhu, Liyan Shi, Jinfeng Wang, Wujun Liu, Yihan Chen, Xiaofeng Weng, Yiqun Liu, Xingwang Ren, Huazhong Identification and Functional Characterization of CsMYCs in Cucumber Glandular Trichome Development |
title | Identification and Functional Characterization of CsMYCs in Cucumber Glandular Trichome Development |
title_full | Identification and Functional Characterization of CsMYCs in Cucumber Glandular Trichome Development |
title_fullStr | Identification and Functional Characterization of CsMYCs in Cucumber Glandular Trichome Development |
title_full_unstemmed | Identification and Functional Characterization of CsMYCs in Cucumber Glandular Trichome Development |
title_short | Identification and Functional Characterization of CsMYCs in Cucumber Glandular Trichome Development |
title_sort | identification and functional characterization of csmycs in cucumber glandular trichome development |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094329/ https://www.ncbi.nlm.nih.gov/pubmed/37047408 http://dx.doi.org/10.3390/ijms24076435 |
work_keys_str_mv | AT fengzhongxuan identificationandfunctionalcharacterizationofcsmycsincucumberglandulartrichomedevelopment AT sunlei identificationandfunctionalcharacterizationofcsmycsincucumberglandulartrichomedevelopment AT dongmingming identificationandfunctionalcharacterizationofcsmycsincucumberglandulartrichomedevelopment AT fanshanshan identificationandfunctionalcharacterizationofcsmycsincucumberglandulartrichomedevelopment AT shikexin identificationandfunctionalcharacterizationofcsmycsincucumberglandulartrichomedevelopment AT quyixin identificationandfunctionalcharacterizationofcsmycsincucumberglandulartrichomedevelopment AT zhuliyan identificationandfunctionalcharacterizationofcsmycsincucumberglandulartrichomedevelopment AT shijinfeng identificationandfunctionalcharacterizationofcsmycsincucumberglandulartrichomedevelopment AT wangwujun identificationandfunctionalcharacterizationofcsmycsincucumberglandulartrichomedevelopment AT liuyihan identificationandfunctionalcharacterizationofcsmycsincucumberglandulartrichomedevelopment AT chenxiaofeng identificationandfunctionalcharacterizationofcsmycsincucumberglandulartrichomedevelopment AT wengyiqun identificationandfunctionalcharacterizationofcsmycsincucumberglandulartrichomedevelopment AT liuxingwang identificationandfunctionalcharacterizationofcsmycsincucumberglandulartrichomedevelopment AT renhuazhong identificationandfunctionalcharacterizationofcsmycsincucumberglandulartrichomedevelopment |