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Long-Term Cryopreservation of Nasal Polyp Tissue in a Biobank for the Isolation and Culture of Primary Epithelial Cells

Epithelial cells may play an important role in the pathologic process of chronic rhinosinusitis with nasal polyps. Therefore, providing epithelial cells from a biobank could greatly contribute to further research. In the present work, the isolation of epithelial cells from long-term cryopreserved ti...

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Autores principales: Kim, Jonghui, Hegener, Karla, Hagedorn, Claudia, Jamal Jameel, Kaschin, Weidinger, Daniel, Seuthe, Inga Marte Charlott, Eichhorn, Sabine, Kreppel, Florian, Knobloch, Jürgen, Park, Jonas Jae-Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094359/
https://www.ncbi.nlm.nih.gov/pubmed/37047354
http://dx.doi.org/10.3390/ijms24076383
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author Kim, Jonghui
Hegener, Karla
Hagedorn, Claudia
Jamal Jameel, Kaschin
Weidinger, Daniel
Seuthe, Inga Marte Charlott
Eichhorn, Sabine
Kreppel, Florian
Knobloch, Jürgen
Park, Jonas Jae-Hyun
author_facet Kim, Jonghui
Hegener, Karla
Hagedorn, Claudia
Jamal Jameel, Kaschin
Weidinger, Daniel
Seuthe, Inga Marte Charlott
Eichhorn, Sabine
Kreppel, Florian
Knobloch, Jürgen
Park, Jonas Jae-Hyun
author_sort Kim, Jonghui
collection PubMed
description Epithelial cells may play an important role in the pathologic process of chronic rhinosinusitis with nasal polyps. Therefore, providing epithelial cells from a biobank could greatly contribute to further research. In the present work, the isolation of epithelial cells from long-term cryopreserved tissue is demonstrated. Polyp tissues were cryopreserved in a commercially available freezing medium with dimethyl sulfoxide and stored in liquid nitrogen. The outgrowth and proliferation of epithelial cells from cryopreserved tissue were evaluated and compared to that of fresh tissue. Flow cytometric analysis with anti-cytokeratin, anti-p63, and anti-Ki-67 was performed to identify epithelial cells and determine differentiation and proliferation. A functionality test was performed by determining type 2–relevant proteins, representatively thymic stromal lymphopoietin (TSLP) and periostin, using ELISA. Primary epithelial cells could be isolated from cryopreserved tissues. Cells from cryopreserved tissues showed comparable outgrowth and proliferation to that of fresh tissue. Isolated epithelial cells showed high cytokeratin, p63, and Ki-67 expression and secreted TSLP and periostin. In the present study, a method for long-term cryopreservation of polyp tissue was established, thereby enabling the isolation and cell culture of primary cell culture at a later time. Epithelial cell availability should be greatly improved by including this method in a biobank.
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spelling pubmed-100943592023-04-13 Long-Term Cryopreservation of Nasal Polyp Tissue in a Biobank for the Isolation and Culture of Primary Epithelial Cells Kim, Jonghui Hegener, Karla Hagedorn, Claudia Jamal Jameel, Kaschin Weidinger, Daniel Seuthe, Inga Marte Charlott Eichhorn, Sabine Kreppel, Florian Knobloch, Jürgen Park, Jonas Jae-Hyun Int J Mol Sci Article Epithelial cells may play an important role in the pathologic process of chronic rhinosinusitis with nasal polyps. Therefore, providing epithelial cells from a biobank could greatly contribute to further research. In the present work, the isolation of epithelial cells from long-term cryopreserved tissue is demonstrated. Polyp tissues were cryopreserved in a commercially available freezing medium with dimethyl sulfoxide and stored in liquid nitrogen. The outgrowth and proliferation of epithelial cells from cryopreserved tissue were evaluated and compared to that of fresh tissue. Flow cytometric analysis with anti-cytokeratin, anti-p63, and anti-Ki-67 was performed to identify epithelial cells and determine differentiation and proliferation. A functionality test was performed by determining type 2–relevant proteins, representatively thymic stromal lymphopoietin (TSLP) and periostin, using ELISA. Primary epithelial cells could be isolated from cryopreserved tissues. Cells from cryopreserved tissues showed comparable outgrowth and proliferation to that of fresh tissue. Isolated epithelial cells showed high cytokeratin, p63, and Ki-67 expression and secreted TSLP and periostin. In the present study, a method for long-term cryopreservation of polyp tissue was established, thereby enabling the isolation and cell culture of primary cell culture at a later time. Epithelial cell availability should be greatly improved by including this method in a biobank. MDPI 2023-03-28 /pmc/articles/PMC10094359/ /pubmed/37047354 http://dx.doi.org/10.3390/ijms24076383 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Jonghui
Hegener, Karla
Hagedorn, Claudia
Jamal Jameel, Kaschin
Weidinger, Daniel
Seuthe, Inga Marte Charlott
Eichhorn, Sabine
Kreppel, Florian
Knobloch, Jürgen
Park, Jonas Jae-Hyun
Long-Term Cryopreservation of Nasal Polyp Tissue in a Biobank for the Isolation and Culture of Primary Epithelial Cells
title Long-Term Cryopreservation of Nasal Polyp Tissue in a Biobank for the Isolation and Culture of Primary Epithelial Cells
title_full Long-Term Cryopreservation of Nasal Polyp Tissue in a Biobank for the Isolation and Culture of Primary Epithelial Cells
title_fullStr Long-Term Cryopreservation of Nasal Polyp Tissue in a Biobank for the Isolation and Culture of Primary Epithelial Cells
title_full_unstemmed Long-Term Cryopreservation of Nasal Polyp Tissue in a Biobank for the Isolation and Culture of Primary Epithelial Cells
title_short Long-Term Cryopreservation of Nasal Polyp Tissue in a Biobank for the Isolation and Culture of Primary Epithelial Cells
title_sort long-term cryopreservation of nasal polyp tissue in a biobank for the isolation and culture of primary epithelial cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094359/
https://www.ncbi.nlm.nih.gov/pubmed/37047354
http://dx.doi.org/10.3390/ijms24076383
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