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Microbial Synthesis of High-Molecular-Weight, Highly Repetitive Protein Polymers

High molecular weight (MW), highly repetitive protein polymers are attractive candidates to replace petroleum-derived materials as these protein-based materials (PBMs) are renewable, biodegradable, and have outstanding mechanical properties. However, their high MW and highly repetitive sequence feat...

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Autores principales: Jeon, Juya, Subramani, Shri Venkatesh, Lee, Kok Zhi, Jiang, Bojing, Zhang, Fuzhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094428/
https://www.ncbi.nlm.nih.gov/pubmed/37047388
http://dx.doi.org/10.3390/ijms24076416
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author Jeon, Juya
Subramani, Shri Venkatesh
Lee, Kok Zhi
Jiang, Bojing
Zhang, Fuzhong
author_facet Jeon, Juya
Subramani, Shri Venkatesh
Lee, Kok Zhi
Jiang, Bojing
Zhang, Fuzhong
author_sort Jeon, Juya
collection PubMed
description High molecular weight (MW), highly repetitive protein polymers are attractive candidates to replace petroleum-derived materials as these protein-based materials (PBMs) are renewable, biodegradable, and have outstanding mechanical properties. However, their high MW and highly repetitive sequence features make them difficult to synthesize in fast-growing microbial cells in sufficient amounts for real applications. To overcome this challenge, various methods were developed to synthesize repetitive PBMs. Here, we review recent strategies in the construction of repetitive genes, expression of repetitive proteins from circular mRNAs, and synthesis of repetitive proteins by ligation and protein polymerization. We discuss the advantages and limitations of each method and highlight future directions that will lead to scalable production of highly repetitive PBMs for a wide range of applications.
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spelling pubmed-100944282023-04-13 Microbial Synthesis of High-Molecular-Weight, Highly Repetitive Protein Polymers Jeon, Juya Subramani, Shri Venkatesh Lee, Kok Zhi Jiang, Bojing Zhang, Fuzhong Int J Mol Sci Review High molecular weight (MW), highly repetitive protein polymers are attractive candidates to replace petroleum-derived materials as these protein-based materials (PBMs) are renewable, biodegradable, and have outstanding mechanical properties. However, their high MW and highly repetitive sequence features make them difficult to synthesize in fast-growing microbial cells in sufficient amounts for real applications. To overcome this challenge, various methods were developed to synthesize repetitive PBMs. Here, we review recent strategies in the construction of repetitive genes, expression of repetitive proteins from circular mRNAs, and synthesis of repetitive proteins by ligation and protein polymerization. We discuss the advantages and limitations of each method and highlight future directions that will lead to scalable production of highly repetitive PBMs for a wide range of applications. MDPI 2023-03-29 /pmc/articles/PMC10094428/ /pubmed/37047388 http://dx.doi.org/10.3390/ijms24076416 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Jeon, Juya
Subramani, Shri Venkatesh
Lee, Kok Zhi
Jiang, Bojing
Zhang, Fuzhong
Microbial Synthesis of High-Molecular-Weight, Highly Repetitive Protein Polymers
title Microbial Synthesis of High-Molecular-Weight, Highly Repetitive Protein Polymers
title_full Microbial Synthesis of High-Molecular-Weight, Highly Repetitive Protein Polymers
title_fullStr Microbial Synthesis of High-Molecular-Weight, Highly Repetitive Protein Polymers
title_full_unstemmed Microbial Synthesis of High-Molecular-Weight, Highly Repetitive Protein Polymers
title_short Microbial Synthesis of High-Molecular-Weight, Highly Repetitive Protein Polymers
title_sort microbial synthesis of high-molecular-weight, highly repetitive protein polymers
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094428/
https://www.ncbi.nlm.nih.gov/pubmed/37047388
http://dx.doi.org/10.3390/ijms24076416
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