Cargando…

Evaluation of the Ion Channel Assembly in a Eukaryotic Cell-Free System Focusing on Two-Pore Domain Potassium Channels K(2P)

Oligomeric ion channels are abundant in nature. However, the recombinant expression in cell culture-based systems remains tedious and challenging due to negative side effects, limiting the understanding of their role in health and disease. Accordingly, in this work, we demonstrate the cell-free synt...

Descripción completa

Detalles Bibliográficos
Autores principales:	Ullrich, Jessica, Ohlhoff, Carsten, Dondapati, Srujan Kumar, Zemella, Anne, Kubick, Stefan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094441/ https://www.ncbi.nlm.nih.gov/pubmed/37047271 http://dx.doi.org/10.3390/ijms24076299

Ejemplares similares

Functional Reconstitution of Membrane Proteins Derived From Eukaryotic Cell-Free Systems
por: Dondapati, Srujan Kumar, et al.
Publicado: (2019)

Enhancing the performance of a mutant pyrrolysyl-tRNA synthetase to create a highly versatile eukaryotic cell-free protein synthesis tool
por: Schloßhauer, Jeffrey L., et al.
Publicado: (2023)

Targeted esterase-induced dye (TED) loading supports direct calcium imaging in eukaryotic cell-free systems
por: Dhandapani, Priyavathi, et al.
Publicado: (2021)

Cell-Free Protein Synthesis: A Promising Option for Future Drug Development
por: Dondapati, Srujan Kumar, et al.
Publicado: (2020)

The Potential of Eukaryotic Cell-Free Systems as a Rapid Response to Novel Zoonotic Pathogens: Analysis of SARS-CoV-2 Viral Proteins
por: Ramm, Franziska, et al.
Publicado: (2022)

Two-pore Domain Potassium Channels in Astrocytes
por: Ryoo, Kanghyun, et al.
Publicado: (2016)

Cell‐free production of pore forming toxins: Functional analysis of thermostable direct hemolysin from Vibrio parahaemolyticus
por: Dondapati, Srujan Kumar, et al.
Publicado: (2017)

Accelerating the Production of Druggable Targets: Eukaryotic Cell-Free Systems Come into Focus
por: Thoring, Lena, et al.
Publicado: (2019)

Potassium channels as multi-ion single-file pores
Publicado: (1978)

Trafficking of Neuronal Two Pore Domain Potassium Channels
por: Mathie, Alistair, et al.
Publicado: (2010)

Two-pore domain potassium channels in the adrenal cortex
por: Bandulik, Sascha, et al.
Publicado: (2014)

Altered Expression of Two-Pore Domain Potassium (K(2P)) Channels in Cancer
por: Williams, Sarah, et al.
Publicado: (2013)

Expression and localisation of two-pore domain (K2P) background leak potassium ion channels in the mouse retina
por: Hughes, Steven, et al.
Publicado: (2017)

Oligomerization of the heteromeric γ-aminobutyric acid receptor GABA(B) in a eukaryotic cell-free system
por: Ullrich, Jessica, et al.
Publicado: (2022)

Two-pore domain potassium channels enable action potential generation in the absence of voltage-gated potassium channels
por: MacKenzie, Georgina, et al.
Publicado: (2014)

Diversity of voltage-gated potassium channels and cyclic nucleotide-binding domain-containing channels in eukaryotes
por: Pozdnyakov, Ilya, et al.
Publicado: (2020)

The pore of voltage-gated potassium ion channels is strained when closed
por: Fowler, Philip W., et al.
Publicado: (2013)

Mammalian cell-free protein expression promotes the functional characterization of the tripartite non-hemolytic enterotoxin from Bacillus cereus
por: Ramm, Franziska, et al.
Publicado: (2020)

Domain assembly of NAADP-gated two-pore channels
por: Churamani, Dev, et al.
Publicado: (2011)

Nonindependent K(+) Movement through the Pore in IRK1 Potassium Channels
por: Stampe, Per, et al.
Publicado: (1998)

Charged pore-lining residues are required for normal channel kinetics in the eukaryotic mechanosensitive ion channel MSL1
por: Schlegel, Angela M., et al.
Publicado: (2020)

Two pore domain potassium channels in cerebral ischemia: a focus on K(2P)9.1 (TASK3, KCNK9)
por: Ehling, Petra, et al.
Publicado: (2010)

The voltage-sensing domain of a phosphatase gates the pore of a potassium channel
por: Arrigoni, Cristina, et al.
Publicado: (2013)

Development of Non-opioid Analgesics Targeting Two-pore Domain Potassium Channels
por: Huang, Lu, et al.
Publicado: (2022)

Electrostatics and the Gating Pore of Shaker Potassium Channels
por: Islas, Leon D., et al.
Publicado: (2001)

Pore size matters for potassium channel conductance
por: Naranjo, David, et al.
Publicado: (2016)

A Mutation in S6 of Shaker Potassium Channels Decreases the K(+) Affinity of an Ion Binding Site Revealing Ion–Ion Interactions in the Pore
por: Ogielska, Eva M., et al.
Publicado: (1998)

Endocytosis as a mode to regulate functional expression of two-pore domain potassium (K(2P)) channels
por: O’Kelly, Ita
Publicado: (2014)

A “Target Class” Screen to Identify Activators of Two-Pore Domain Potassium (K2P) Channels
por: McCoull, David, et al.
Publicado: (2020)

Effective pore size and radius of capture for K(+) ions in K-channels
por: Moldenhauer, Hans, et al.
Publicado: (2016)

Correction: Two pore domain potassium channels in cerebral ischemia: a focus on K2P9.1 (TASK3, KCNK9)
por: Ehling, Petra, et al.
Publicado: (2013)

Cell-Free Protein Synthesis: Pros and Cons of Prokaryotic and Eukaryotic Systems
por: Zemella, Anne, et al.
Publicado: (2015)

A Specific Two-pore Domain Potassium Channel Blocker Defines the Structure of the TASK-1 Open Pore
por: Streit, Anne K., et al.
Publicado: (2011)

Contribution of Neuronal and Glial Two-Pore-Domain Potassium Channels in Health and Neurological Disorders
por: Luo, Yuncheng, et al.
Publicado: (2021)

Advances in the Understanding of Two-Pore Domain TASK Potassium Channels and Their Potential as Therapeutic Targets
por: Fan, Xueming, et al.
Publicado: (2022)

Two-Pore-Domain Potassium (K(2P)-) Channels: Cardiac Expression Patterns and Disease-Specific Remodelling Processes
por: Wiedmann, Felix, et al.
Publicado: (2021)

Ion permeation in potassium ion channels
por: Coates, Leighton
Publicado: (2020)

Long-pore Electrostatics in Inward-rectifier Potassium Channels
por: Robertson, Janice L., et al.
Publicado: (2008)

Tandem pore TWIK-related potassium channels and neuroprotection
por: Lamas, J. Antonio, et al.
Publicado: (2019)

Pore-forming transmembrane domains control ion selectivity and selectivity filter conformation in the KirBac1.1 potassium channel
por: Matamoros, Marcos, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_e0047ls2vqs5df0067bqqkg1nt