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Crystal Structure of Pyrrolysyl-tRNA Synthetase from a Methanogenic Archaeon ISO4-G1 and Its Structure-Based Engineering for Highly-Productive Cell-Free Genetic Code Expansion with Non-Canonical Amino Acids

Pairs of pyrrolysyl-tRNA synthetase (PylRS) and tRNA(Pyl) from Methanosarcina mazei and Methanosarcina barkeri are widely used for site-specific incorporations of non-canonical amino acids into proteins (genetic code expansion). Previously, we achieved full productivity of cell-free protein synthesi...

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Detalles Bibliográficos
Autores principales:	Yanagisawa, Tatsuo, Seki, Eiko, Tanabe, Hiroaki, Fujii, Yoshifumi, Sakamoto, Kensaku, Yokoyama, Shigeyuki
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094482/ https://www.ncbi.nlm.nih.gov/pubmed/37047230 http://dx.doi.org/10.3390/ijms24076256

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