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Lysophosphatidylinositol Promotes Chemotaxis and Cytokine Synthesis in Mast Cells with Differential Participation of GPR55 and CB2 Receptors

Mast cells (MCs) are the main participants in the control of immune reactions associated with inflammation, allergies, defense against pathogens, and tumor growth. Bioactive lipids are lipophilic compounds able to modulate MC activation. Here, we explored some of the effects of the bioactive lipid l...

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Autores principales: Martínez-Aguilar, Lizbeth Magnolia, Ibarra-Sánchez, Alfredo, Guerrero-Morán, Daniel José, Macías-Silva, Marina, Muñoz-Bello, Jesús Omar, Padilla, Alejandro, Lizano, Marcela, González-Espinosa, Claudia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094727/
https://www.ncbi.nlm.nih.gov/pubmed/37047288
http://dx.doi.org/10.3390/ijms24076316
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author Martínez-Aguilar, Lizbeth Magnolia
Ibarra-Sánchez, Alfredo
Guerrero-Morán, Daniel José
Macías-Silva, Marina
Muñoz-Bello, Jesús Omar
Padilla, Alejandro
Lizano, Marcela
González-Espinosa, Claudia
author_facet Martínez-Aguilar, Lizbeth Magnolia
Ibarra-Sánchez, Alfredo
Guerrero-Morán, Daniel José
Macías-Silva, Marina
Muñoz-Bello, Jesús Omar
Padilla, Alejandro
Lizano, Marcela
González-Espinosa, Claudia
author_sort Martínez-Aguilar, Lizbeth Magnolia
collection PubMed
description Mast cells (MCs) are the main participants in the control of immune reactions associated with inflammation, allergies, defense against pathogens, and tumor growth. Bioactive lipids are lipophilic compounds able to modulate MC activation. Here, we explored some of the effects of the bioactive lipid lysophosphatidylinositol (LPI) on MCs. Utilizing murine bone marrow-derived mast cells (BMMCs), we found that LPI did not cause degranulation, but slightly increased FcεRI-dependent β-hexosaminidase release. However, LPI induced strong chemotaxis together with changes in LIM kinase (LIMK) and cofilin phosphorylation. LPI also promoted modifications to actin cytoskeleton dynamics that were detected by an increase in cell size and interruptions in the continuity of the cortical actin ring. The chemotaxis and cortical actin ring changes were dependent on GPR55 receptor activation, since the specific agonist O1602 mimicked the effects of LPI and the selective antagonist ML193 prevented them. The LPI and O1602-dependent stimulation of BMMC also led to VEGF, TNF, IL-1α, and IL-1β mRNA accumulation, but, in contrast with chemotaxis-related processes, the effects on cytokine transcription were dependent on GPR55 and cannabinoid (CB) 2 receptors, since they were sensitive to ML193 and to the specific CB2 receptor antagonist AM630. Remarkably, GPR55-dependent BMMC chemotaxis was observed towards conditioned media from distinct mouse and human cancer cells. Our data suggest that LPI induces the chemotaxis of MCs and leads to cytokine production in MC in vitro with the differential participation of GPR55 and CB2 receptors. These effects could play a significant role in the recruitment of MCs to tumors and the production of MC-derived pro-angiogenic factors in the tumor microenvironment.
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spelling pubmed-100947272023-04-13 Lysophosphatidylinositol Promotes Chemotaxis and Cytokine Synthesis in Mast Cells with Differential Participation of GPR55 and CB2 Receptors Martínez-Aguilar, Lizbeth Magnolia Ibarra-Sánchez, Alfredo Guerrero-Morán, Daniel José Macías-Silva, Marina Muñoz-Bello, Jesús Omar Padilla, Alejandro Lizano, Marcela González-Espinosa, Claudia Int J Mol Sci Article Mast cells (MCs) are the main participants in the control of immune reactions associated with inflammation, allergies, defense against pathogens, and tumor growth. Bioactive lipids are lipophilic compounds able to modulate MC activation. Here, we explored some of the effects of the bioactive lipid lysophosphatidylinositol (LPI) on MCs. Utilizing murine bone marrow-derived mast cells (BMMCs), we found that LPI did not cause degranulation, but slightly increased FcεRI-dependent β-hexosaminidase release. However, LPI induced strong chemotaxis together with changes in LIM kinase (LIMK) and cofilin phosphorylation. LPI also promoted modifications to actin cytoskeleton dynamics that were detected by an increase in cell size and interruptions in the continuity of the cortical actin ring. The chemotaxis and cortical actin ring changes were dependent on GPR55 receptor activation, since the specific agonist O1602 mimicked the effects of LPI and the selective antagonist ML193 prevented them. The LPI and O1602-dependent stimulation of BMMC also led to VEGF, TNF, IL-1α, and IL-1β mRNA accumulation, but, in contrast with chemotaxis-related processes, the effects on cytokine transcription were dependent on GPR55 and cannabinoid (CB) 2 receptors, since they were sensitive to ML193 and to the specific CB2 receptor antagonist AM630. Remarkably, GPR55-dependent BMMC chemotaxis was observed towards conditioned media from distinct mouse and human cancer cells. Our data suggest that LPI induces the chemotaxis of MCs and leads to cytokine production in MC in vitro with the differential participation of GPR55 and CB2 receptors. These effects could play a significant role in the recruitment of MCs to tumors and the production of MC-derived pro-angiogenic factors in the tumor microenvironment. MDPI 2023-03-28 /pmc/articles/PMC10094727/ /pubmed/37047288 http://dx.doi.org/10.3390/ijms24076316 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Martínez-Aguilar, Lizbeth Magnolia
Ibarra-Sánchez, Alfredo
Guerrero-Morán, Daniel José
Macías-Silva, Marina
Muñoz-Bello, Jesús Omar
Padilla, Alejandro
Lizano, Marcela
González-Espinosa, Claudia
Lysophosphatidylinositol Promotes Chemotaxis and Cytokine Synthesis in Mast Cells with Differential Participation of GPR55 and CB2 Receptors
title Lysophosphatidylinositol Promotes Chemotaxis and Cytokine Synthesis in Mast Cells with Differential Participation of GPR55 and CB2 Receptors
title_full Lysophosphatidylinositol Promotes Chemotaxis and Cytokine Synthesis in Mast Cells with Differential Participation of GPR55 and CB2 Receptors
title_fullStr Lysophosphatidylinositol Promotes Chemotaxis and Cytokine Synthesis in Mast Cells with Differential Participation of GPR55 and CB2 Receptors
title_full_unstemmed Lysophosphatidylinositol Promotes Chemotaxis and Cytokine Synthesis in Mast Cells with Differential Participation of GPR55 and CB2 Receptors
title_short Lysophosphatidylinositol Promotes Chemotaxis and Cytokine Synthesis in Mast Cells with Differential Participation of GPR55 and CB2 Receptors
title_sort lysophosphatidylinositol promotes chemotaxis and cytokine synthesis in mast cells with differential participation of gpr55 and cb2 receptors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094727/
https://www.ncbi.nlm.nih.gov/pubmed/37047288
http://dx.doi.org/10.3390/ijms24076316
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