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Dynamic Changes and Characterization of the Metal Ions in the Silk Glands and Silk Fibers of Silkworm
Metal ions are involved in the conformational transition of silk fibroin and influence the structure and mechanical properties of silk fibers. However, the dynamic characteristics of metal ions during the formation of silk fibers remain unclear. In this study, we found that the silk glands of silkwo...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094808/ https://www.ncbi.nlm.nih.gov/pubmed/37047527 http://dx.doi.org/10.3390/ijms24076556 |
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author | Liu, Qingsong Wang, Xin Zhou, Yifan Tan, Xiaoyin Xie, Xiaoqian Li, Yi Dong, Haonan Tang, Zhangchen Zhao, Ping Xia, Qingyou |
author_facet | Liu, Qingsong Wang, Xin Zhou, Yifan Tan, Xiaoyin Xie, Xiaoqian Li, Yi Dong, Haonan Tang, Zhangchen Zhao, Ping Xia, Qingyou |
author_sort | Liu, Qingsong |
collection | PubMed |
description | Metal ions are involved in the conformational transition of silk fibroin and influence the structure and mechanical properties of silk fibers. However, the dynamic characteristics of metal ions during the formation of silk fibers remain unclear. In this study, we found that the silk glands of silkworms contain various metal elements, with varying levels of the metal elements in different zones of the glands and higher levels in the anterior silk glands. Additionally, the content of various metallic elements in the silk glands varied greatly before and after spinning, similar to their content in different cocoon layers, thus, indicating that the anterior silk glands maintain a certain metal ion environment for the transport and conformational transformation of the silk proteins. Most of the metallic elements located in fibroin were confirmed using degumming experiments. For the first time, a scanning electron microscope energy spectrometry system was used to characterize the metal elements in the cross-section of silk and cocoons. These findings have deepened our understanding of the relationship between the overall metal ion environment and silk fiber formation and help us further conceptualize the utilization of metal ions as targets to improve the mechanical properties of the silk fibers. |
format | Online Article Text |
id | pubmed-10094808 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100948082023-04-13 Dynamic Changes and Characterization of the Metal Ions in the Silk Glands and Silk Fibers of Silkworm Liu, Qingsong Wang, Xin Zhou, Yifan Tan, Xiaoyin Xie, Xiaoqian Li, Yi Dong, Haonan Tang, Zhangchen Zhao, Ping Xia, Qingyou Int J Mol Sci Article Metal ions are involved in the conformational transition of silk fibroin and influence the structure and mechanical properties of silk fibers. However, the dynamic characteristics of metal ions during the formation of silk fibers remain unclear. In this study, we found that the silk glands of silkworms contain various metal elements, with varying levels of the metal elements in different zones of the glands and higher levels in the anterior silk glands. Additionally, the content of various metallic elements in the silk glands varied greatly before and after spinning, similar to their content in different cocoon layers, thus, indicating that the anterior silk glands maintain a certain metal ion environment for the transport and conformational transformation of the silk proteins. Most of the metallic elements located in fibroin were confirmed using degumming experiments. For the first time, a scanning electron microscope energy spectrometry system was used to characterize the metal elements in the cross-section of silk and cocoons. These findings have deepened our understanding of the relationship between the overall metal ion environment and silk fiber formation and help us further conceptualize the utilization of metal ions as targets to improve the mechanical properties of the silk fibers. MDPI 2023-03-31 /pmc/articles/PMC10094808/ /pubmed/37047527 http://dx.doi.org/10.3390/ijms24076556 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liu, Qingsong Wang, Xin Zhou, Yifan Tan, Xiaoyin Xie, Xiaoqian Li, Yi Dong, Haonan Tang, Zhangchen Zhao, Ping Xia, Qingyou Dynamic Changes and Characterization of the Metal Ions in the Silk Glands and Silk Fibers of Silkworm |
title | Dynamic Changes and Characterization of the Metal Ions in the Silk Glands and Silk Fibers of Silkworm |
title_full | Dynamic Changes and Characterization of the Metal Ions in the Silk Glands and Silk Fibers of Silkworm |
title_fullStr | Dynamic Changes and Characterization of the Metal Ions in the Silk Glands and Silk Fibers of Silkworm |
title_full_unstemmed | Dynamic Changes and Characterization of the Metal Ions in the Silk Glands and Silk Fibers of Silkworm |
title_short | Dynamic Changes and Characterization of the Metal Ions in the Silk Glands and Silk Fibers of Silkworm |
title_sort | dynamic changes and characterization of the metal ions in the silk glands and silk fibers of silkworm |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094808/ https://www.ncbi.nlm.nih.gov/pubmed/37047527 http://dx.doi.org/10.3390/ijms24076556 |
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