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Molecular Cytological Analysis and Specific Marker Development in Wheat-Psathyrostachys huashanica Keng 3Ns Additional Line with Elongated Glume

Psathyrostachys huashanica Keng (2n = 2x = 14, NsNs) is an excellent gene resource for wheat breeding, which is characterized by early maturity, low plant height, and disease resistance. The wheat-P. huashanica derivatives were created by the elite genes of P. huashanica and permeate into common whe...

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Autores principales: Pang, Jingyu, Huang, Chenxi, Wang, Yuesheng, Wen, Xinyu, Deng, Pingchuan, Li, Tingdong, Wang, Changyou, Liu, Xinlun, Chen, Chunhuan, Zhao, Jixin, Ji, Wanquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094845/
https://www.ncbi.nlm.nih.gov/pubmed/37047699
http://dx.doi.org/10.3390/ijms24076726
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author Pang, Jingyu
Huang, Chenxi
Wang, Yuesheng
Wen, Xinyu
Deng, Pingchuan
Li, Tingdong
Wang, Changyou
Liu, Xinlun
Chen, Chunhuan
Zhao, Jixin
Ji, Wanquan
author_facet Pang, Jingyu
Huang, Chenxi
Wang, Yuesheng
Wen, Xinyu
Deng, Pingchuan
Li, Tingdong
Wang, Changyou
Liu, Xinlun
Chen, Chunhuan
Zhao, Jixin
Ji, Wanquan
author_sort Pang, Jingyu
collection PubMed
description Psathyrostachys huashanica Keng (2n = 2x = 14, NsNs) is an excellent gene resource for wheat breeding, which is characterized by early maturity, low plant height, and disease resistance. The wheat-P. huashanica derivatives were created by the elite genes of P. huashanica and permeate into common wheat through hybridization. Among them, a long-glume material 20JH1155 was identified, with larger grains and longer spike than its parents. In the present study, the methods of cytological observation, GISH, and sequential FISH analysis showed that 20JH1155 contained 21 pairs of wheat chromosomes and a pair of P. huashanica. There were some differences in 5A and 7B chromosomes between 20JH1155 and parental wheat 7182. Molecular marker, FISH, and sequence cloning indicated 20JH1155 alien chromosomes were 3Ns of P. huashanica. In addition, differentially expressed genes during immature spikelet development of 20JH1155 and 7182 and predicted transcription factors were obtained by transcriptome sequencing. Moreover, a total of 7 makers derived from Ph#3Ns were developed from transcriptome data. Taken together, the wheat-P. huashanica derived line 20JH1155 provides a new horizon on distant hybridization of wheat and accelerates the utilization of genes of P. huashanica.
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spelling pubmed-100948452023-04-13 Molecular Cytological Analysis and Specific Marker Development in Wheat-Psathyrostachys huashanica Keng 3Ns Additional Line with Elongated Glume Pang, Jingyu Huang, Chenxi Wang, Yuesheng Wen, Xinyu Deng, Pingchuan Li, Tingdong Wang, Changyou Liu, Xinlun Chen, Chunhuan Zhao, Jixin Ji, Wanquan Int J Mol Sci Article Psathyrostachys huashanica Keng (2n = 2x = 14, NsNs) is an excellent gene resource for wheat breeding, which is characterized by early maturity, low plant height, and disease resistance. The wheat-P. huashanica derivatives were created by the elite genes of P. huashanica and permeate into common wheat through hybridization. Among them, a long-glume material 20JH1155 was identified, with larger grains and longer spike than its parents. In the present study, the methods of cytological observation, GISH, and sequential FISH analysis showed that 20JH1155 contained 21 pairs of wheat chromosomes and a pair of P. huashanica. There were some differences in 5A and 7B chromosomes between 20JH1155 and parental wheat 7182. Molecular marker, FISH, and sequence cloning indicated 20JH1155 alien chromosomes were 3Ns of P. huashanica. In addition, differentially expressed genes during immature spikelet development of 20JH1155 and 7182 and predicted transcription factors were obtained by transcriptome sequencing. Moreover, a total of 7 makers derived from Ph#3Ns were developed from transcriptome data. Taken together, the wheat-P. huashanica derived line 20JH1155 provides a new horizon on distant hybridization of wheat and accelerates the utilization of genes of P. huashanica. MDPI 2023-04-04 /pmc/articles/PMC10094845/ /pubmed/37047699 http://dx.doi.org/10.3390/ijms24076726 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pang, Jingyu
Huang, Chenxi
Wang, Yuesheng
Wen, Xinyu
Deng, Pingchuan
Li, Tingdong
Wang, Changyou
Liu, Xinlun
Chen, Chunhuan
Zhao, Jixin
Ji, Wanquan
Molecular Cytological Analysis and Specific Marker Development in Wheat-Psathyrostachys huashanica Keng 3Ns Additional Line with Elongated Glume
title Molecular Cytological Analysis and Specific Marker Development in Wheat-Psathyrostachys huashanica Keng 3Ns Additional Line with Elongated Glume
title_full Molecular Cytological Analysis and Specific Marker Development in Wheat-Psathyrostachys huashanica Keng 3Ns Additional Line with Elongated Glume
title_fullStr Molecular Cytological Analysis and Specific Marker Development in Wheat-Psathyrostachys huashanica Keng 3Ns Additional Line with Elongated Glume
title_full_unstemmed Molecular Cytological Analysis and Specific Marker Development in Wheat-Psathyrostachys huashanica Keng 3Ns Additional Line with Elongated Glume
title_short Molecular Cytological Analysis and Specific Marker Development in Wheat-Psathyrostachys huashanica Keng 3Ns Additional Line with Elongated Glume
title_sort molecular cytological analysis and specific marker development in wheat-psathyrostachys huashanica keng 3ns additional line with elongated glume
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094845/
https://www.ncbi.nlm.nih.gov/pubmed/37047699
http://dx.doi.org/10.3390/ijms24076726
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