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Cathepsin-L Secreted by High-Quality Bovine Embryos Exerts an Embryotrophic Effect In Vitro

While human in vitro embryo production is generally performed individually, animal models have shown that culturing embryos in groups improves blastocyst yield and quality. Paracrine embryotrophins could be responsible for this improved embryo development, but their identity remains largely unknown....

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Autores principales: Raes, Annelies, Wydooghe, Eline, Pavani, Krishna Chaitanya, Bogado Pascottini, Osvaldo, Van Steendam, Katleen, Dhaenens, Maarten, Boel, Annekatrien, Heras, Sonia, Heindryckx, Björn, Peelman, Luc, Deforce, Dieter, Van Nieuwerburgh, Filip, Opsomer, Geert, Van Soom, Ann, Smits, Katrien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094850/
https://www.ncbi.nlm.nih.gov/pubmed/37047535
http://dx.doi.org/10.3390/ijms24076563
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author Raes, Annelies
Wydooghe, Eline
Pavani, Krishna Chaitanya
Bogado Pascottini, Osvaldo
Van Steendam, Katleen
Dhaenens, Maarten
Boel, Annekatrien
Heras, Sonia
Heindryckx, Björn
Peelman, Luc
Deforce, Dieter
Van Nieuwerburgh, Filip
Opsomer, Geert
Van Soom, Ann
Smits, Katrien
author_facet Raes, Annelies
Wydooghe, Eline
Pavani, Krishna Chaitanya
Bogado Pascottini, Osvaldo
Van Steendam, Katleen
Dhaenens, Maarten
Boel, Annekatrien
Heras, Sonia
Heindryckx, Björn
Peelman, Luc
Deforce, Dieter
Van Nieuwerburgh, Filip
Opsomer, Geert
Van Soom, Ann
Smits, Katrien
author_sort Raes, Annelies
collection PubMed
description While human in vitro embryo production is generally performed individually, animal models have shown that culturing embryos in groups improves blastocyst yield and quality. Paracrine embryotrophins could be responsible for this improved embryo development, but their identity remains largely unknown. We hypothesize that supplementation of embryotrophic proteins to a culture medium could be the key to improve individual embryo production. In this study, proteomics screening of culture media conditioned by bovine embryos revealed cathepsin-L as being secreted by both excellent- and good-quality embryos, while being absent in the medium conditioned by poor-quality embryos. The embryotrophic role of cathepsin-L was explored in vitro, whereby bovine zygotes were cultured individually for 8 days with or without cathepsin-L. Preliminary dose–response experiments pointed out 100 ng/mL as the optimal concentration of cathepsin-L in embryo culture medium. Supplementation of cathepsin-L to individual culture systems significantly improved blastocyst development and quality in terms of blastocoel formation at day 7, and the hatching ratio and apoptotic cell ratio at day 8, compared to the control. Taken together, cathepsin-L acts as an important embryotrophin by increasing embryo quality, and regulating blastulation and hatching in bovine in vitro embryo production.
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spelling pubmed-100948502023-04-13 Cathepsin-L Secreted by High-Quality Bovine Embryos Exerts an Embryotrophic Effect In Vitro Raes, Annelies Wydooghe, Eline Pavani, Krishna Chaitanya Bogado Pascottini, Osvaldo Van Steendam, Katleen Dhaenens, Maarten Boel, Annekatrien Heras, Sonia Heindryckx, Björn Peelman, Luc Deforce, Dieter Van Nieuwerburgh, Filip Opsomer, Geert Van Soom, Ann Smits, Katrien Int J Mol Sci Article While human in vitro embryo production is generally performed individually, animal models have shown that culturing embryos in groups improves blastocyst yield and quality. Paracrine embryotrophins could be responsible for this improved embryo development, but their identity remains largely unknown. We hypothesize that supplementation of embryotrophic proteins to a culture medium could be the key to improve individual embryo production. In this study, proteomics screening of culture media conditioned by bovine embryos revealed cathepsin-L as being secreted by both excellent- and good-quality embryos, while being absent in the medium conditioned by poor-quality embryos. The embryotrophic role of cathepsin-L was explored in vitro, whereby bovine zygotes were cultured individually for 8 days with or without cathepsin-L. Preliminary dose–response experiments pointed out 100 ng/mL as the optimal concentration of cathepsin-L in embryo culture medium. Supplementation of cathepsin-L to individual culture systems significantly improved blastocyst development and quality in terms of blastocoel formation at day 7, and the hatching ratio and apoptotic cell ratio at day 8, compared to the control. Taken together, cathepsin-L acts as an important embryotrophin by increasing embryo quality, and regulating blastulation and hatching in bovine in vitro embryo production. MDPI 2023-03-31 /pmc/articles/PMC10094850/ /pubmed/37047535 http://dx.doi.org/10.3390/ijms24076563 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Raes, Annelies
Wydooghe, Eline
Pavani, Krishna Chaitanya
Bogado Pascottini, Osvaldo
Van Steendam, Katleen
Dhaenens, Maarten
Boel, Annekatrien
Heras, Sonia
Heindryckx, Björn
Peelman, Luc
Deforce, Dieter
Van Nieuwerburgh, Filip
Opsomer, Geert
Van Soom, Ann
Smits, Katrien
Cathepsin-L Secreted by High-Quality Bovine Embryos Exerts an Embryotrophic Effect In Vitro
title Cathepsin-L Secreted by High-Quality Bovine Embryos Exerts an Embryotrophic Effect In Vitro
title_full Cathepsin-L Secreted by High-Quality Bovine Embryos Exerts an Embryotrophic Effect In Vitro
title_fullStr Cathepsin-L Secreted by High-Quality Bovine Embryos Exerts an Embryotrophic Effect In Vitro
title_full_unstemmed Cathepsin-L Secreted by High-Quality Bovine Embryos Exerts an Embryotrophic Effect In Vitro
title_short Cathepsin-L Secreted by High-Quality Bovine Embryos Exerts an Embryotrophic Effect In Vitro
title_sort cathepsin-l secreted by high-quality bovine embryos exerts an embryotrophic effect in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094850/
https://www.ncbi.nlm.nih.gov/pubmed/37047535
http://dx.doi.org/10.3390/ijms24076563
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