MRC-5 Human Lung Fibroblasts Alleviate the Genotoxic Effect of Fe-N Co-Doped Titanium Dioxide Nanoparticles through an OGG1/2-Dependent Reparatory Mechanism

The current study was focused on the potential of pure P25 TiO(2) nanoparticles (NPs) and Fe(1%)-N co-doped P25 TiO(2) NPs to induce cyto- and genotoxic effects in MRC-5 human pulmonary fibroblasts. The oxidative lesions of P25 NPs were reflected in the amount of 8-hydroxydeoxyguanosine accumulated...

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Detalles Bibliográficos
Autores principales: Miu, Bogdan Andrei, Voinea, Ionela Cristina, Diamandescu, Lucian, Dinischiotu, Anca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094865/
https://www.ncbi.nlm.nih.gov/pubmed/37047374
http://dx.doi.org/10.3390/ijms24076401
Descripción
Sumario:The current study was focused on the potential of pure P25 TiO(2) nanoparticles (NPs) and Fe(1%)-N co-doped P25 TiO(2) NPs to induce cyto- and genotoxic effects in MRC-5 human pulmonary fibroblasts. The oxidative lesions of P25 NPs were reflected in the amount of 8-hydroxydeoxyguanosine accumulated in DNA and the lysosomal damage produced, but iron-doping partially suppressed these effects. However, neither P25 nor Fe(1%)-N co-doped P25 NPs had such a serious effect of inducing DNA fragmentation or activating apoptosis signaling. Moreover, oxo-guanine glycosylase 1/2, a key enzyme of the base excision repair mechanism, was overexpressed in response to the oxidative DNA deterioration induced by P25 and P25-Fe(1%)-N NPs.

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