MRC-5 Human Lung Fibroblasts Alleviate the Genotoxic Effect of Fe-N Co-Doped Titanium Dioxide Nanoparticles through an OGG1/2-Dependent Reparatory Mechanism

The current study was focused on the potential of pure P25 TiO(2) nanoparticles (NPs) and Fe(1%)-N co-doped P25 TiO(2) NPs to induce cyto- and genotoxic effects in MRC-5 human pulmonary fibroblasts. The oxidative lesions of P25 NPs were reflected in the amount of 8-hydroxydeoxyguanosine accumulated...

Descripción completa

Detalles Bibliográficos
Autores principales: Miu, Bogdan Andrei, Voinea, Ionela Cristina, Diamandescu, Lucian, Dinischiotu, Anca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094865/
https://www.ncbi.nlm.nih.gov/pubmed/37047374
http://dx.doi.org/10.3390/ijms24076401
_version_ 1785023943846920192
author Miu, Bogdan Andrei
Voinea, Ionela Cristina
Diamandescu, Lucian
Dinischiotu, Anca
author_facet Miu, Bogdan Andrei
Voinea, Ionela Cristina
Diamandescu, Lucian
Dinischiotu, Anca
author_sort Miu, Bogdan Andrei
collection PubMed
description The current study was focused on the potential of pure P25 TiO(2) nanoparticles (NPs) and Fe(1%)-N co-doped P25 TiO(2) NPs to induce cyto- and genotoxic effects in MRC-5 human pulmonary fibroblasts. The oxidative lesions of P25 NPs were reflected in the amount of 8-hydroxydeoxyguanosine accumulated in DNA and the lysosomal damage produced, but iron-doping partially suppressed these effects. However, neither P25 nor Fe(1%)-N co-doped P25 NPs had such a serious effect of inducing DNA fragmentation or activating apoptosis signaling. Moreover, oxo-guanine glycosylase 1/2, a key enzyme of the base excision repair mechanism, was overexpressed in response to the oxidative DNA deterioration induced by P25 and P25-Fe(1%)-N NPs.
format Online
Article
Text
id pubmed-10094865
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-100948652023-04-13 MRC-5 Human Lung Fibroblasts Alleviate the Genotoxic Effect of Fe-N Co-Doped Titanium Dioxide Nanoparticles through an OGG1/2-Dependent Reparatory Mechanism Miu, Bogdan Andrei Voinea, Ionela Cristina Diamandescu, Lucian Dinischiotu, Anca Int J Mol Sci Article The current study was focused on the potential of pure P25 TiO(2) nanoparticles (NPs) and Fe(1%)-N co-doped P25 TiO(2) NPs to induce cyto- and genotoxic effects in MRC-5 human pulmonary fibroblasts. The oxidative lesions of P25 NPs were reflected in the amount of 8-hydroxydeoxyguanosine accumulated in DNA and the lysosomal damage produced, but iron-doping partially suppressed these effects. However, neither P25 nor Fe(1%)-N co-doped P25 NPs had such a serious effect of inducing DNA fragmentation or activating apoptosis signaling. Moreover, oxo-guanine glycosylase 1/2, a key enzyme of the base excision repair mechanism, was overexpressed in response to the oxidative DNA deterioration induced by P25 and P25-Fe(1%)-N NPs. MDPI 2023-03-29 /pmc/articles/PMC10094865/ /pubmed/37047374 http://dx.doi.org/10.3390/ijms24076401 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Miu, Bogdan Andrei
Voinea, Ionela Cristina
Diamandescu, Lucian
Dinischiotu, Anca
MRC-5 Human Lung Fibroblasts Alleviate the Genotoxic Effect of Fe-N Co-Doped Titanium Dioxide Nanoparticles through an OGG1/2-Dependent Reparatory Mechanism
title MRC-5 Human Lung Fibroblasts Alleviate the Genotoxic Effect of Fe-N Co-Doped Titanium Dioxide Nanoparticles through an OGG1/2-Dependent Reparatory Mechanism
title_full MRC-5 Human Lung Fibroblasts Alleviate the Genotoxic Effect of Fe-N Co-Doped Titanium Dioxide Nanoparticles through an OGG1/2-Dependent Reparatory Mechanism
title_fullStr MRC-5 Human Lung Fibroblasts Alleviate the Genotoxic Effect of Fe-N Co-Doped Titanium Dioxide Nanoparticles through an OGG1/2-Dependent Reparatory Mechanism
title_full_unstemmed MRC-5 Human Lung Fibroblasts Alleviate the Genotoxic Effect of Fe-N Co-Doped Titanium Dioxide Nanoparticles through an OGG1/2-Dependent Reparatory Mechanism
title_short MRC-5 Human Lung Fibroblasts Alleviate the Genotoxic Effect of Fe-N Co-Doped Titanium Dioxide Nanoparticles through an OGG1/2-Dependent Reparatory Mechanism
title_sort mrc-5 human lung fibroblasts alleviate the genotoxic effect of fe-n co-doped titanium dioxide nanoparticles through an ogg1/2-dependent reparatory mechanism
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094865/
https://www.ncbi.nlm.nih.gov/pubmed/37047374
http://dx.doi.org/10.3390/ijms24076401
work_keys_str_mv AT miubogdanandrei mrc5humanlungfibroblastsalleviatethegenotoxiceffectoffencodopedtitaniumdioxidenanoparticlesthroughanogg12dependentreparatorymechanism
AT voineaionelacristina mrc5humanlungfibroblastsalleviatethegenotoxiceffectoffencodopedtitaniumdioxidenanoparticlesthroughanogg12dependentreparatorymechanism
AT diamandesculucian mrc5humanlungfibroblastsalleviatethegenotoxiceffectoffencodopedtitaniumdioxidenanoparticlesthroughanogg12dependentreparatorymechanism
AT dinischiotuanca mrc5humanlungfibroblastsalleviatethegenotoxiceffectoffencodopedtitaniumdioxidenanoparticlesthroughanogg12dependentreparatorymechanism

Ejemplares similares