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Diagnostic Aqueous Humor Proteome Predicts Metastatic Potential in Uveal Melanoma
Gene expression profiling (GEP) is clinically validated to stratify the risk of metastasis by assigning uveal melanoma (UM) patients to two highly prognostic molecular classes: class 1 (low metastatic risk) and class 2 (high metastatic risk). However, GEP requires intraocular tumor biopsy, which is...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094875/ https://www.ncbi.nlm.nih.gov/pubmed/37047796 http://dx.doi.org/10.3390/ijms24076825 |
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author | Peng, Chen-Ching Sirivolu, Shreya Pike, Sarah Kim, Mary E. Reiser, Bibiana Li, Hong-Tao Liang, Gangning Xu, Liya Berry, Jesse L. |
author_facet | Peng, Chen-Ching Sirivolu, Shreya Pike, Sarah Kim, Mary E. Reiser, Bibiana Li, Hong-Tao Liang, Gangning Xu, Liya Berry, Jesse L. |
author_sort | Peng, Chen-Ching |
collection | PubMed |
description | Gene expression profiling (GEP) is clinically validated to stratify the risk of metastasis by assigning uveal melanoma (UM) patients to two highly prognostic molecular classes: class 1 (low metastatic risk) and class 2 (high metastatic risk). However, GEP requires intraocular tumor biopsy, which is limited by small tumor size and tumor heterogeneity; furthermore, there are small risks of retinal hemorrhage, bleeding, or tumor dissemination. Thus, ocular liquid biopsy has emerged as a less-invasive alternative. In this study, we seek to determine the aqueous humor (AH) proteome related to the advanced GEP class 2 using diagnostic AH liquid biopsy specimens. Twenty AH samples were collected from patients with UM, grouped by GEP classes. Protein expression levels of 1472 targets were analyzed, compared between GEP classes, and correlated with clinical features. Significant differentially expressed proteins (DEPs) were subjected to analysis for cellular pathway and upstream regulator identification. The results showed that 45 DEPs detected in the AH could differentiate GEP class 1 and 2 at diagnosis. IL1R and SPRY2 are potential upstream regulators for the 8/45 DEPs that contribute to metastasis-related pathways. AH liquid biopsy offers a new opportunity to determine metastatic potential for patients in the absence of tumor biopsy. |
format | Online Article Text |
id | pubmed-10094875 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100948752023-04-13 Diagnostic Aqueous Humor Proteome Predicts Metastatic Potential in Uveal Melanoma Peng, Chen-Ching Sirivolu, Shreya Pike, Sarah Kim, Mary E. Reiser, Bibiana Li, Hong-Tao Liang, Gangning Xu, Liya Berry, Jesse L. Int J Mol Sci Article Gene expression profiling (GEP) is clinically validated to stratify the risk of metastasis by assigning uveal melanoma (UM) patients to two highly prognostic molecular classes: class 1 (low metastatic risk) and class 2 (high metastatic risk). However, GEP requires intraocular tumor biopsy, which is limited by small tumor size and tumor heterogeneity; furthermore, there are small risks of retinal hemorrhage, bleeding, or tumor dissemination. Thus, ocular liquid biopsy has emerged as a less-invasive alternative. In this study, we seek to determine the aqueous humor (AH) proteome related to the advanced GEP class 2 using diagnostic AH liquid biopsy specimens. Twenty AH samples were collected from patients with UM, grouped by GEP classes. Protein expression levels of 1472 targets were analyzed, compared between GEP classes, and correlated with clinical features. Significant differentially expressed proteins (DEPs) were subjected to analysis for cellular pathway and upstream regulator identification. The results showed that 45 DEPs detected in the AH could differentiate GEP class 1 and 2 at diagnosis. IL1R and SPRY2 are potential upstream regulators for the 8/45 DEPs that contribute to metastasis-related pathways. AH liquid biopsy offers a new opportunity to determine metastatic potential for patients in the absence of tumor biopsy. MDPI 2023-04-06 /pmc/articles/PMC10094875/ /pubmed/37047796 http://dx.doi.org/10.3390/ijms24076825 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Peng, Chen-Ching Sirivolu, Shreya Pike, Sarah Kim, Mary E. Reiser, Bibiana Li, Hong-Tao Liang, Gangning Xu, Liya Berry, Jesse L. Diagnostic Aqueous Humor Proteome Predicts Metastatic Potential in Uveal Melanoma |
title | Diagnostic Aqueous Humor Proteome Predicts Metastatic Potential in Uveal Melanoma |
title_full | Diagnostic Aqueous Humor Proteome Predicts Metastatic Potential in Uveal Melanoma |
title_fullStr | Diagnostic Aqueous Humor Proteome Predicts Metastatic Potential in Uveal Melanoma |
title_full_unstemmed | Diagnostic Aqueous Humor Proteome Predicts Metastatic Potential in Uveal Melanoma |
title_short | Diagnostic Aqueous Humor Proteome Predicts Metastatic Potential in Uveal Melanoma |
title_sort | diagnostic aqueous humor proteome predicts metastatic potential in uveal melanoma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094875/ https://www.ncbi.nlm.nih.gov/pubmed/37047796 http://dx.doi.org/10.3390/ijms24076825 |
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