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OsLUX Confers Rice Cold Tolerance as a Positive Regulatory Factor
During the early seedling stage, rice (Oryza sativa L.) must overcome low-temperature stress. While a few cold-tolerance genes have been characterized, further excavation of cold-resistance genes is still needed. In this study, we identified a cold-induced transcription factor—LUX ARRHYTHMO (LUX)—in...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094877/ https://www.ncbi.nlm.nih.gov/pubmed/37047700 http://dx.doi.org/10.3390/ijms24076727 |
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author | Huang, Peng Ding, Zhengquan Duan, Min Xiong, Yi Li, Xinxin Yuan, Xi Huang, Ji |
author_facet | Huang, Peng Ding, Zhengquan Duan, Min Xiong, Yi Li, Xinxin Yuan, Xi Huang, Ji |
author_sort | Huang, Peng |
collection | PubMed |
description | During the early seedling stage, rice (Oryza sativa L.) must overcome low-temperature stress. While a few cold-tolerance genes have been characterized, further excavation of cold-resistance genes is still needed. In this study, we identified a cold-induced transcription factor—LUX ARRHYTHMO (LUX)—in rice. OsLUX was found to be specifically expressed in leaf blades and upregulated by both cold stress and circadian rhythm. The full-length OsLUX showed autoactivation activity, and the OsLUX protein localized throughout the entire onion cell. Overexpressing OsLUX resulted in increased cold tolerance and reduced ion leakage under cold-stress conditions during the seedling stage. In contrast, the knockout of OsLUX decreased seedling cold tolerance and showed higher ion leakage compared to the wild type. Furthermore, overexpressing OsLUX upregulated the expression levels of oxidative stress-responsive genes, which improved reactive oxygen species (ROS) scavenging ability and enhanced tolerance to chilling stress. Promoter analysis showed that the OsLUX promoter contains two dehydration-responsive element binding (DREB) motifs at positions −510/−505 (GTCGGa) and −162/−170 (cCACCGccc), which indicated that OsDREB1s and OsDREB2s probably regulate OsLUX expression by binding to the motif to respond to cold stress. Thus, OsLUX may act as a downstream gene of the DREB pathway. These results demonstrate that OsLUX serves as a positive regulatory factor of cold stress and that overexpressing OsLUX could be used in rice breeding programs to enhance abiotic stress tolerance. |
format | Online Article Text |
id | pubmed-10094877 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100948772023-04-13 OsLUX Confers Rice Cold Tolerance as a Positive Regulatory Factor Huang, Peng Ding, Zhengquan Duan, Min Xiong, Yi Li, Xinxin Yuan, Xi Huang, Ji Int J Mol Sci Article During the early seedling stage, rice (Oryza sativa L.) must overcome low-temperature stress. While a few cold-tolerance genes have been characterized, further excavation of cold-resistance genes is still needed. In this study, we identified a cold-induced transcription factor—LUX ARRHYTHMO (LUX)—in rice. OsLUX was found to be specifically expressed in leaf blades and upregulated by both cold stress and circadian rhythm. The full-length OsLUX showed autoactivation activity, and the OsLUX protein localized throughout the entire onion cell. Overexpressing OsLUX resulted in increased cold tolerance and reduced ion leakage under cold-stress conditions during the seedling stage. In contrast, the knockout of OsLUX decreased seedling cold tolerance and showed higher ion leakage compared to the wild type. Furthermore, overexpressing OsLUX upregulated the expression levels of oxidative stress-responsive genes, which improved reactive oxygen species (ROS) scavenging ability and enhanced tolerance to chilling stress. Promoter analysis showed that the OsLUX promoter contains two dehydration-responsive element binding (DREB) motifs at positions −510/−505 (GTCGGa) and −162/−170 (cCACCGccc), which indicated that OsDREB1s and OsDREB2s probably regulate OsLUX expression by binding to the motif to respond to cold stress. Thus, OsLUX may act as a downstream gene of the DREB pathway. These results demonstrate that OsLUX serves as a positive regulatory factor of cold stress and that overexpressing OsLUX could be used in rice breeding programs to enhance abiotic stress tolerance. MDPI 2023-04-04 /pmc/articles/PMC10094877/ /pubmed/37047700 http://dx.doi.org/10.3390/ijms24076727 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Huang, Peng Ding, Zhengquan Duan, Min Xiong, Yi Li, Xinxin Yuan, Xi Huang, Ji OsLUX Confers Rice Cold Tolerance as a Positive Regulatory Factor |
title | OsLUX Confers Rice Cold Tolerance as a Positive Regulatory Factor |
title_full | OsLUX Confers Rice Cold Tolerance as a Positive Regulatory Factor |
title_fullStr | OsLUX Confers Rice Cold Tolerance as a Positive Regulatory Factor |
title_full_unstemmed | OsLUX Confers Rice Cold Tolerance as a Positive Regulatory Factor |
title_short | OsLUX Confers Rice Cold Tolerance as a Positive Regulatory Factor |
title_sort | oslux confers rice cold tolerance as a positive regulatory factor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094877/ https://www.ncbi.nlm.nih.gov/pubmed/37047700 http://dx.doi.org/10.3390/ijms24076727 |
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