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Biofilm-Based Biocatalysis for Galactooligosaccharides Production by the Surface Display of β-Galactosidase in Pichia pastoris

Galactooligosaccharides (GOS) are one of the most important functional oligosaccharide prebiotics. The surface display of enzymes was considered one of the most excellent strategies to obtain these products. However, a rough industrial environment would affect the biocatalytic process. The catalytic...

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Autores principales: Chen, Tianpeng, Wang, Shimeng, Niu, Huanqing, Yang, Guanjia, Wang, Sinan, Wang, Yuqi, Zhou, Chaowei, Yu, Bin, Yang, Pengpeng, Sun, Wenjun, Liu, Dong, Ying, Hanjie, Chen, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094928/
https://www.ncbi.nlm.nih.gov/pubmed/37047479
http://dx.doi.org/10.3390/ijms24076507
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author Chen, Tianpeng
Wang, Shimeng
Niu, Huanqing
Yang, Guanjia
Wang, Sinan
Wang, Yuqi
Zhou, Chaowei
Yu, Bin
Yang, Pengpeng
Sun, Wenjun
Liu, Dong
Ying, Hanjie
Chen, Yong
author_facet Chen, Tianpeng
Wang, Shimeng
Niu, Huanqing
Yang, Guanjia
Wang, Sinan
Wang, Yuqi
Zhou, Chaowei
Yu, Bin
Yang, Pengpeng
Sun, Wenjun
Liu, Dong
Ying, Hanjie
Chen, Yong
author_sort Chen, Tianpeng
collection PubMed
description Galactooligosaccharides (GOS) are one of the most important functional oligosaccharide prebiotics. The surface display of enzymes was considered one of the most excellent strategies to obtain these products. However, a rough industrial environment would affect the biocatalytic process. The catalytic process could be efficiently improved using biofilm-based fermentation with high resistance and activity. Therefore, the combination of the surface display of β-galactosidase and biofilm formation in Pichia pastoris was constructed. The results showed that the catalytic conversion rate of GOS was up to 50.3% with the maximum enzyme activity of 5125 U/g by screening the anchorin, and the number of the continuous catalysis batches was up to 23 times. Thus, surface display based on biofilm-immobilized fermentation integrated catalysis and growth was a co-culture system, such that a dynamic equilibrium in the consolidated integrative process was achieved. This study provides the basis for developing biofilm-based surface display methods in P. pastoris during biochemical production processes.
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spelling pubmed-100949282023-04-13 Biofilm-Based Biocatalysis for Galactooligosaccharides Production by the Surface Display of β-Galactosidase in Pichia pastoris Chen, Tianpeng Wang, Shimeng Niu, Huanqing Yang, Guanjia Wang, Sinan Wang, Yuqi Zhou, Chaowei Yu, Bin Yang, Pengpeng Sun, Wenjun Liu, Dong Ying, Hanjie Chen, Yong Int J Mol Sci Article Galactooligosaccharides (GOS) are one of the most important functional oligosaccharide prebiotics. The surface display of enzymes was considered one of the most excellent strategies to obtain these products. However, a rough industrial environment would affect the biocatalytic process. The catalytic process could be efficiently improved using biofilm-based fermentation with high resistance and activity. Therefore, the combination of the surface display of β-galactosidase and biofilm formation in Pichia pastoris was constructed. The results showed that the catalytic conversion rate of GOS was up to 50.3% with the maximum enzyme activity of 5125 U/g by screening the anchorin, and the number of the continuous catalysis batches was up to 23 times. Thus, surface display based on biofilm-immobilized fermentation integrated catalysis and growth was a co-culture system, such that a dynamic equilibrium in the consolidated integrative process was achieved. This study provides the basis for developing biofilm-based surface display methods in P. pastoris during biochemical production processes. MDPI 2023-03-30 /pmc/articles/PMC10094928/ /pubmed/37047479 http://dx.doi.org/10.3390/ijms24076507 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Tianpeng
Wang, Shimeng
Niu, Huanqing
Yang, Guanjia
Wang, Sinan
Wang, Yuqi
Zhou, Chaowei
Yu, Bin
Yang, Pengpeng
Sun, Wenjun
Liu, Dong
Ying, Hanjie
Chen, Yong
Biofilm-Based Biocatalysis for Galactooligosaccharides Production by the Surface Display of β-Galactosidase in Pichia pastoris
title Biofilm-Based Biocatalysis for Galactooligosaccharides Production by the Surface Display of β-Galactosidase in Pichia pastoris
title_full Biofilm-Based Biocatalysis for Galactooligosaccharides Production by the Surface Display of β-Galactosidase in Pichia pastoris
title_fullStr Biofilm-Based Biocatalysis for Galactooligosaccharides Production by the Surface Display of β-Galactosidase in Pichia pastoris
title_full_unstemmed Biofilm-Based Biocatalysis for Galactooligosaccharides Production by the Surface Display of β-Galactosidase in Pichia pastoris
title_short Biofilm-Based Biocatalysis for Galactooligosaccharides Production by the Surface Display of β-Galactosidase in Pichia pastoris
title_sort biofilm-based biocatalysis for galactooligosaccharides production by the surface display of β-galactosidase in pichia pastoris
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094928/
https://www.ncbi.nlm.nih.gov/pubmed/37047479
http://dx.doi.org/10.3390/ijms24076507
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