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Extracellular Vesicles and Their Zeta Potential as Future Markers Associated with Nutrition and Molecular Biomarkers in Breast Cancer

A nutritional intervention promotes the loss of body and visceral fat while maintaining muscle mass in breast cancer patients. Extracellular vesicles (EVs) and their characteristics can be potential biomarkers of disease. Here, we explore the changes in the Zeta potential of EVs; the content of miRN...

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Autores principales: Mendivil-Alvarado, Herminia, Limon-Miro, Ana Teresa, Carvajal-Millan, Elizabeth, Lizardi-Mendoza, Jaime, Mercado-Lara, Araceli, Coronado-Alvarado, Carlos D., Rascón-Durán, María L., Anduro-Corona, Iván, Talamás-Lara, Daniel, Rascón-Careaga, Antonio, Astiazarán-García, Humberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094966/
https://www.ncbi.nlm.nih.gov/pubmed/37047783
http://dx.doi.org/10.3390/ijms24076810
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author Mendivil-Alvarado, Herminia
Limon-Miro, Ana Teresa
Carvajal-Millan, Elizabeth
Lizardi-Mendoza, Jaime
Mercado-Lara, Araceli
Coronado-Alvarado, Carlos D.
Rascón-Durán, María L.
Anduro-Corona, Iván
Talamás-Lara, Daniel
Rascón-Careaga, Antonio
Astiazarán-García, Humberto
author_facet Mendivil-Alvarado, Herminia
Limon-Miro, Ana Teresa
Carvajal-Millan, Elizabeth
Lizardi-Mendoza, Jaime
Mercado-Lara, Araceli
Coronado-Alvarado, Carlos D.
Rascón-Durán, María L.
Anduro-Corona, Iván
Talamás-Lara, Daniel
Rascón-Careaga, Antonio
Astiazarán-García, Humberto
author_sort Mendivil-Alvarado, Herminia
collection PubMed
description A nutritional intervention promotes the loss of body and visceral fat while maintaining muscle mass in breast cancer patients. Extracellular vesicles (EVs) and their characteristics can be potential biomarkers of disease. Here, we explore the changes in the Zeta potential of EVs; the content of miRNA-30, miRNA-145, and miRNA-155; and their association with body composition and biomarkers of metabolic risk in breast cancer patients, before and 6 months after a nutritional intervention. Clinicopathological data (HER2neu, estrogen receptor, and Ki67), anthropometric and body composition data, and plasma samples were available from a previous study. Plasma EVs were isolated and characterized in 16 patients. The expression of miRNA-30, miRNA-145, and miRNA-155 was analyzed. The Zeta potential was associated with HER2neu (β = 2.1; p = 0.00), Ki67 (β = −1.39; p = 0.007), estrogen positive (β = 1.57; p = 0.01), weight (β = −0.09; p = 0.00), and visceral fat (β = 0.004; p = 0.00). miRNA-30 was associated with LDL (β = −0.012; p = 0.01) and HDL (β = −0.02; p = 0.05). miRNA-155 was associated with visceral fat (β = −0.0007; p = 0.05) and Ki67 (β = −0.47; p = 0.04). Our results reveal significant associations between the expression of miRNA-30 and miRNA-155 and the Zeta potential of the EVs with biomarkers of metabolic risk and disease prognosis in women with breast cancer; particularly, the Zeta potential of EVs can be a new biomarker sensitive to changes in the nutritional status and breast cancer progression.
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spelling pubmed-100949662023-04-13 Extracellular Vesicles and Their Zeta Potential as Future Markers Associated with Nutrition and Molecular Biomarkers in Breast Cancer Mendivil-Alvarado, Herminia Limon-Miro, Ana Teresa Carvajal-Millan, Elizabeth Lizardi-Mendoza, Jaime Mercado-Lara, Araceli Coronado-Alvarado, Carlos D. Rascón-Durán, María L. Anduro-Corona, Iván Talamás-Lara, Daniel Rascón-Careaga, Antonio Astiazarán-García, Humberto Int J Mol Sci Article A nutritional intervention promotes the loss of body and visceral fat while maintaining muscle mass in breast cancer patients. Extracellular vesicles (EVs) and their characteristics can be potential biomarkers of disease. Here, we explore the changes in the Zeta potential of EVs; the content of miRNA-30, miRNA-145, and miRNA-155; and their association with body composition and biomarkers of metabolic risk in breast cancer patients, before and 6 months after a nutritional intervention. Clinicopathological data (HER2neu, estrogen receptor, and Ki67), anthropometric and body composition data, and plasma samples were available from a previous study. Plasma EVs were isolated and characterized in 16 patients. The expression of miRNA-30, miRNA-145, and miRNA-155 was analyzed. The Zeta potential was associated with HER2neu (β = 2.1; p = 0.00), Ki67 (β = −1.39; p = 0.007), estrogen positive (β = 1.57; p = 0.01), weight (β = −0.09; p = 0.00), and visceral fat (β = 0.004; p = 0.00). miRNA-30 was associated with LDL (β = −0.012; p = 0.01) and HDL (β = −0.02; p = 0.05). miRNA-155 was associated with visceral fat (β = −0.0007; p = 0.05) and Ki67 (β = −0.47; p = 0.04). Our results reveal significant associations between the expression of miRNA-30 and miRNA-155 and the Zeta potential of the EVs with biomarkers of metabolic risk and disease prognosis in women with breast cancer; particularly, the Zeta potential of EVs can be a new biomarker sensitive to changes in the nutritional status and breast cancer progression. MDPI 2023-04-06 /pmc/articles/PMC10094966/ /pubmed/37047783 http://dx.doi.org/10.3390/ijms24076810 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mendivil-Alvarado, Herminia
Limon-Miro, Ana Teresa
Carvajal-Millan, Elizabeth
Lizardi-Mendoza, Jaime
Mercado-Lara, Araceli
Coronado-Alvarado, Carlos D.
Rascón-Durán, María L.
Anduro-Corona, Iván
Talamás-Lara, Daniel
Rascón-Careaga, Antonio
Astiazarán-García, Humberto
Extracellular Vesicles and Their Zeta Potential as Future Markers Associated with Nutrition and Molecular Biomarkers in Breast Cancer
title Extracellular Vesicles and Their Zeta Potential as Future Markers Associated with Nutrition and Molecular Biomarkers in Breast Cancer
title_full Extracellular Vesicles and Their Zeta Potential as Future Markers Associated with Nutrition and Molecular Biomarkers in Breast Cancer
title_fullStr Extracellular Vesicles and Their Zeta Potential as Future Markers Associated with Nutrition and Molecular Biomarkers in Breast Cancer
title_full_unstemmed Extracellular Vesicles and Their Zeta Potential as Future Markers Associated with Nutrition and Molecular Biomarkers in Breast Cancer
title_short Extracellular Vesicles and Their Zeta Potential as Future Markers Associated with Nutrition and Molecular Biomarkers in Breast Cancer
title_sort extracellular vesicles and their zeta potential as future markers associated with nutrition and molecular biomarkers in breast cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094966/
https://www.ncbi.nlm.nih.gov/pubmed/37047783
http://dx.doi.org/10.3390/ijms24076810
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