Cargando…
Blood–Brain Barrier Biomarkers before and after Kidney Transplantation
Kidney transplantation (KT) may improve the neurological status of chronic kidney disease (CKD) patients, reflected by the altered levels of circulating BBB-specific biomarkers. This study compares the levels of neuron specific enolase (NSE), brain-derived neurotrophic factor (BDNF), neurofilament l...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10095132/ https://www.ncbi.nlm.nih.gov/pubmed/37047601 http://dx.doi.org/10.3390/ijms24076628 |
_version_ | 1785024009531817984 |
---|---|
author | Hernandez, Leah Ward, Liam J. Arefin, Samsul Barany, Peter Wennberg, Lars Söderberg, Magnus Bruno, Stefania Cantaluppi, Vincenzo Stenvinkel, Peter Kublickiene, Karolina |
author_facet | Hernandez, Leah Ward, Liam J. Arefin, Samsul Barany, Peter Wennberg, Lars Söderberg, Magnus Bruno, Stefania Cantaluppi, Vincenzo Stenvinkel, Peter Kublickiene, Karolina |
author_sort | Hernandez, Leah |
collection | PubMed |
description | Kidney transplantation (KT) may improve the neurological status of chronic kidney disease (CKD) patients, reflected by the altered levels of circulating BBB-specific biomarkers. This study compares the levels of neuron specific enolase (NSE), brain-derived neurotrophic factor (BDNF), neurofilament light chain (NfL), and circulating plasma extracellular vesicles (EVs) in kidney-failure patients before KT and at a two-year follow up. Using ELISA, NSE, BDNF, and NfL levels were measured in the plasma of 74 living-donor KT patients. Plasma EVs were isolated with ultracentrifugation, and characterized for concentration/size and surface protein expression using flow cytometry from a subset of 25 patients. Lower NSE levels, and higher BDNF and NfL were observed at the two-year follow-up compared to the baseline (p < 0.05). Male patients had significantly higher BDNF levels compared to those of females. BBB biomarkers correlated with the baseline lipid profile and with glucose, vitamin D, and inflammation markers after KT. BBB surrogate marker changes in the microcirculation of early vascular aging phenotype patients with calcification and/or fibrosis were observed only in NSE and BDNF. CD31+ microparticles from endothelial cells expressing inflammatory markers such as CD40 and integrins were significantly reduced after KT. KT may, thus, improve the neurological status of CKD patients, as reflected by changes in BBB-specific biomarkers. |
format | Online Article Text |
id | pubmed-10095132 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100951322023-04-13 Blood–Brain Barrier Biomarkers before and after Kidney Transplantation Hernandez, Leah Ward, Liam J. Arefin, Samsul Barany, Peter Wennberg, Lars Söderberg, Magnus Bruno, Stefania Cantaluppi, Vincenzo Stenvinkel, Peter Kublickiene, Karolina Int J Mol Sci Article Kidney transplantation (KT) may improve the neurological status of chronic kidney disease (CKD) patients, reflected by the altered levels of circulating BBB-specific biomarkers. This study compares the levels of neuron specific enolase (NSE), brain-derived neurotrophic factor (BDNF), neurofilament light chain (NfL), and circulating plasma extracellular vesicles (EVs) in kidney-failure patients before KT and at a two-year follow up. Using ELISA, NSE, BDNF, and NfL levels were measured in the plasma of 74 living-donor KT patients. Plasma EVs were isolated with ultracentrifugation, and characterized for concentration/size and surface protein expression using flow cytometry from a subset of 25 patients. Lower NSE levels, and higher BDNF and NfL were observed at the two-year follow-up compared to the baseline (p < 0.05). Male patients had significantly higher BDNF levels compared to those of females. BBB biomarkers correlated with the baseline lipid profile and with glucose, vitamin D, and inflammation markers after KT. BBB surrogate marker changes in the microcirculation of early vascular aging phenotype patients with calcification and/or fibrosis were observed only in NSE and BDNF. CD31+ microparticles from endothelial cells expressing inflammatory markers such as CD40 and integrins were significantly reduced after KT. KT may, thus, improve the neurological status of CKD patients, as reflected by changes in BBB-specific biomarkers. MDPI 2023-04-01 /pmc/articles/PMC10095132/ /pubmed/37047601 http://dx.doi.org/10.3390/ijms24076628 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hernandez, Leah Ward, Liam J. Arefin, Samsul Barany, Peter Wennberg, Lars Söderberg, Magnus Bruno, Stefania Cantaluppi, Vincenzo Stenvinkel, Peter Kublickiene, Karolina Blood–Brain Barrier Biomarkers before and after Kidney Transplantation |
title | Blood–Brain Barrier Biomarkers before and after Kidney Transplantation |
title_full | Blood–Brain Barrier Biomarkers before and after Kidney Transplantation |
title_fullStr | Blood–Brain Barrier Biomarkers before and after Kidney Transplantation |
title_full_unstemmed | Blood–Brain Barrier Biomarkers before and after Kidney Transplantation |
title_short | Blood–Brain Barrier Biomarkers before and after Kidney Transplantation |
title_sort | blood–brain barrier biomarkers before and after kidney transplantation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10095132/ https://www.ncbi.nlm.nih.gov/pubmed/37047601 http://dx.doi.org/10.3390/ijms24076628 |
work_keys_str_mv | AT hernandezleah bloodbrainbarrierbiomarkersbeforeandafterkidneytransplantation AT wardliamj bloodbrainbarrierbiomarkersbeforeandafterkidneytransplantation AT arefinsamsul bloodbrainbarrierbiomarkersbeforeandafterkidneytransplantation AT baranypeter bloodbrainbarrierbiomarkersbeforeandafterkidneytransplantation AT wennberglars bloodbrainbarrierbiomarkersbeforeandafterkidneytransplantation AT soderbergmagnus bloodbrainbarrierbiomarkersbeforeandafterkidneytransplantation AT brunostefania bloodbrainbarrierbiomarkersbeforeandafterkidneytransplantation AT cantaluppivincenzo bloodbrainbarrierbiomarkersbeforeandafterkidneytransplantation AT stenvinkelpeter bloodbrainbarrierbiomarkersbeforeandafterkidneytransplantation AT kublickienekarolina bloodbrainbarrierbiomarkersbeforeandafterkidneytransplantation |